Berberine Attenuates NLRP3 Inflammasome Activation in Macrophages to Reduce the Secretion of Interleukin-1 beta

作者

Jiang, YG;Huang, K;Lin, XX;Chen, QC;Lin, SY;Feng, XM;Zhen, CH;Huang, M;Wang, SM

作者单位

[Jiang, Yugang] Shandong Univ, Div Gen Surg, Shandong Prov Hosp, Shandong, Peoples R China.;-;[Huang, Kai; Lin, Xixia; Chen, Qinchang; Zhen, Chaohui; Huang, Min] Sun Yat Sen Univ, Div Vasc & Thyroid Surg, Sun Yat Sen Mem Hosp, Guangzhou, Guangdong, Peoples R China.;-;[Lin, Shaoyan] Chinese Acad Med Sci, Natl Canc Ctr, Canc Hosp, Beijing, Peoples R China.;-;[Lin, Shaoyan] Peking Union Med Coll, Beijing, Peoples R China.;-;[Feng, Xiaomin; Wang, Shenming] Sun Yat Sen Univ, Affiliated Hosp 1, Div Vasc Surg, Guangzhou, Guangdong, Peoples R China.

摘要

Objectives. The purpose of this study is to evaluate whether Berberine can suppress the inflammatory response in atherosclerosis lesions and its potential associated signaling pathways and mechanism of action. Methods. We isolated human peripheral blood mononuclear cells. After co-culturing them with ox-LDL stimulated cells, ROS was measured by its fluorescence intensity and NADPH oxidase activity was detected by the OD value from the spectrophotometer. Human peripheral blood mononuclear cells were then pretreated with different concentrations of berberine after treatment with NLRP3 activator ATP. Western blot was used to measure the releas of IL-1 beta. We also used confocal microscopy to detect the nuclear import of NF-kB in macrophages. Results. In this study we observed that berberine suppressed IL-1 beta secretion that was induced by the activation of the NLRP3 inflammasome in macrophages. In addition, we demonstrated that berberine may possibly reduce reactive oxygen species (ROS)-dependent NLRP3 inflammasome activation. Moreover, Berberine inhibited the expression of pro-IL-1 beta through inhibition of the nuclear factor kappa b (NF-kappa B) pathway, which prevented the priming IL-1 beta secretion. Conclusion. Our results suggest that berberine alleviates NLRP3 inflammation activation by reducing IL-1 beta secretion from macrophages, which could be an important therapeutic target in atherosclerosis.

关键词

NALP3 INFLAMMASOME; ENDOTHELIAL FUNCTION; OXIDATIVE STRESS; AMYLOID-BETA; ATHEROSCLEROSIS; EXPRESSION; MICROPARTICLES; RECOGNITION; INHIBITION; MECHANISMS

基本信息

所属机构:保健外科

归属医师: 姜雨刚

UT：000418335400010

刊名:ANNALS OF CLINICAL AND LABORATORY SCIENCE

年，卷(期):2017年47卷6期

页码：720-728

附件：

收录: SCIE