Increased expression of microRNA-31-5p inhibits cell proliferation, migration, and invasion via regulating Sp1 transcription factor in HepG2 hepatocellular carcinoma cell line

作者

Zhao, GL;Han, CY;Zhang, Z;Wang, L;Xu, J

作者单位

[Zhao, Guoliang; Han, Chuangye; Xu, Jing] Guangxi Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Nanning 530021, Guangxi Provinc, Peoples R China.;-;[Zhang, Zhi] First Peoples Hosp Nanning, Dept Hepatobiliary Surg, Nanning 530022, Guangxi Provinc, Peoples R China.;-;[Wang, Lei] Shandong Prov Hosp, Dept Ultrasound, Jinan 250021, Shandong, Peoples R China.

摘要

Accumulating evidence has suggested that microRNA-31-5p (miR-31-5p) is dysfunctional in hepatocellular carcinoma (HCC). However, the molecular mechanism of HCC remains unclear. In this study, we investigated the role of miR-31-5p in tumor formation and development of HCC. The expression of miR-31-5p was detected in HCC tissues, corresponding adjacent tissues, normal liver tissues, and HCC cell lines. miR-31-5p mimics and an inhibitor were transfected into HepG2 cells to assess the effects of miR-31-5p on cell proliferation, apoptosis, cell cycle, migration, and invasion assays. Western blotting was used to detect the expression of Sp1 transcription factor (SP1), cyclin D1, and survivin in transfected HCC cells and control cells. The expression of miR-31-5p was significantly decreased in HCC cells and HCC tissues. Overexpression of miR-31-5p inhibited HCC cell growth, migration, and invasion. Overexpression of miR-31-5p reduced the expression of SP1 and cyclin D1, and knockdown of SP1 decreased cyclin Dl expression. The dual luciferase assay showed that miR-31-5p directly targeted SP1 in HepG2. Together, the results suggested that miR-31-5p acted as a tumor suppressor to regulate SP1, and that miR-31-5p could be used as a therapeutic target for the treatment of HCC. (C) 2017 The Authors. Published by Elsevier Inc.

关键词

CANCER CELLS; BREAST-CANCER; TUMOR-SUPPRESSOR; C-MYC; MIR-31; CARCINOGENESIS; METASTASIS; ONCOGENE; PATHWAYS; PROTEIN

基本信息

所属机构:

归属医师: 王蕾

PMID：28623129

UT：000406646600045

刊名:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

年，卷(期):2017年490卷2期

页码：371-377

DOI：10.1016/j.bbrc.2017.06.050

附件： pdf

收录: SCIE