Rho-kinase signaling pathway promotes the expression of PARP to accelerate cardiomyocyte apoptosis in ischemia/reperfusion
作者
Bian, HJ;Zhou, Y;Yu, B;Shang, DY;Liu, FL;Li, B;Qi, JN
作者单位
[Bian, Hongjun; Zhou, Yi; Yu, Bin; Shang, Deya; Liu, Fuli] Shandong Univ, Shandong Prov Hosp, Dept Emergency, Jinan 250021, Shandong, Peoples R China.;-;[Li, Bin] Shandong Univ, Jinan Cent Hosp, Dept Hlth Care, Jinan 250013, Shandong, Peoples R China.;-;[Qi, Jianni] Shandong Univ, Shandong Prov Hosp, Cent Lab, 324 Jingwu Rd, Jinan 250021, Shandong, Peoples R China.
摘要
It has been previously reported that Rho-kinase (ROCK) and poly ADP-ribose polymerase (PARP) serve critical roles in myocardial ischemia/reperfusion (I/R) injury. Studies have additionally demonstrated that the activation of ROCK and the expression of PARP are increased in I/R. However, the effect and mechanism of the two proteins remains to be fully elucidated in I/R. In addition, whether they can be influenced by each other is unclear. In the present study, it was demonstrated that ischemia followed by reperfusion resulted in a significant increase in ROCK and PARP. In addition, Y-27632 (ROCK inhibitor) and 3-aminobenzamide (3-AB; PARP inhibitor) pretreatment rescued myocardial infarction size and cardiomyocyte apoptosis. The inhibitory role of Y-27632 was observed to be superior to that of the 3-AB group. In addition, Y-27632 and 3-AB diminished extracellular signal-related kinase (ERK) phosphorylation and the production of tumor necrosis factor a and interleukin 6. Overall, the results of the present study suggested that the inhibition of ROCK leads to reduced myocardial infarction size and cardiomyocyte apoptosis via the PARP/ERK signaling pathway.
关键词
MEDIATED AIF TRANSLOCATION; POLY(ADP-RIBOSE) POLYMERASE-1; REPERFUSION; INHIBITION; INJURY; CELLS; RAT
基本信息
