Allicin protects auditory hair cells and spiral ganglion neurons from cisplatin - Induced apoptosis
作者
Wu, XM;Li, XF;Song, YD;Li, H;Bai, XH;Liu, WW;Han, YC;Xu, L;Li, JF;Zhang, DG;Wang, HB;Fan, ZM
作者单位
[Wu, Xianmin; Li, Xiaofei; Song, Yongdong; Li, He; Han, Yuechen; Xu, Lei; Zhang, Daogong; Wang, Haibo; Fan, Zhaomin] Shandong Univ, Shandong Prov Hosp, Dept Otolaryngol Head & Neck Surg, Jinan, Peoples R China.;-;[Li, Xiaofei; Song, Yongdong; Bai, Xiaohui; Liu, Wenwen; Li, Jianfeng; Wang, Haibo] Shandong Prov Key Lab Otol, Jinan, Peoples R China.;-;[Li, Xiaofei; Bai, Xiaohui; Liu, Wenwen; Li, Jianfeng; Wang, Haibo] Shandong Univ, Shandong Prov Hosp, Inst Eye & ENT, Jinan, Peoples R China.;-;[Wu, Xianmin; Li, He] Wenzhou Med Univ, Affiliated Hosp 1, Dept Otolaryngol Head & Neck Surg, Wenzhou, Peoples R China.
摘要
Cisplatin is a broad-spectrum anticancer drug that is commonly used in the clinic. Ototoxicity is one of the major side effects of this drug, which caused irreversible sensorineural hearing loss. Allicin, the main biologically active compound derived from garlic, has been shown to exert various anti-apoptotic and anti-oxidative activities in vitro and in vivo studies. We took advantage of C57 mice intraperitoneally injected with cisplatin alone or with cisplatin and allicin combined, to investigate whether allicin plays a protective role in vivo against cisplatin ototoxicity. The result showed that C57 mice in cisplatin group exhibited increased shift in auditory brainstem response, whereas the auditory fuction of mice in allicin + cisplatin group was protected in most frequencies, which was accordance with observed damages of outer hair cells (OHCs) and spiral ganglion neurons (SGNs) in the cochlea. Allicin markedly protected SGN mitochondria from damage and releasing cytochrome c, and significantly reduced pro-apoptosis factor expressions activated by cisplatin, including Bax, cleaved-caspase-9, cleaved-caspase-3and p53. Furthermore, allicin reduced the level of Malondialdehyde (MDA), but increased the level of superoxide dismutase (SOD). All data suggested that allicin could prevent hearing loss induced by cisplatin effectively, of which allicin protected SGNs from apoptosis via mitochondria' pathway while protected OHCs and supporting cells (SCs) from apoptosis through p53 pathway. (C) 2017 Elsevier Ltd. All rights reserved.
关键词
INDUCED OTOTOXICITY; ANTIOXIDANT SYSTEM; OXIDATIVE STRESS; IN-VITRO; INJURY; DEATH; RAT; MECHANISMS; PATHWAY; DYSFUNCTION
基本信息
