HCRP1 inhibits TGF-beta induced epithelial-mesenchymal transition in hepatocellular carcinoma

作者

Yang, W;Wang, JG;Xu, JW;Zhou, DM;Ren, KH;Hou, CJ;Chen, LL;Liu, XP

作者单位

[Yang, Wenlin; Wang, Ji-Gang; Zhou, Danmei; Ren, Kehan; Hou, Chenjian; Liu, Xiuping] Fudan Univ, Sch Basic Med Sci, Dept Pathol, 138 Yixueyuan Rd, Shanghai 200032, Peoples R China.;-;[Wang, Ji-Gang] Qingdao Univ, Dept Pathol, Affiliated Hosp, Qingdao 266003, Shandong, Peoples R China.;-;[Xu, Jiawen] Shangdong Univ, Dept Pathol, Shangdong Prov Hosp, Jinan 250021, Shandong, Peoples R China.;-;[Chen, Linlin; Liu, Xiuping] Fudan Univ, Dept Pathol, Hosp Shanghai 5, Shanghai 200240, Peoples R China.

摘要

Hepatocellular carcinoma-related protein 1 (HCRP1), also known as human vacuolar protein sorting 37 homologue A (hVps37A), has not been detected or is significantly downregulated in hepatocellular carcinoma (HCC) tissues. However, information on the regulatory mechanisms of HCRP1 in HCC remains unclear. Here we found that the downregulation of HCRP1 in HepG2 cells (with low invasion capacity) significantly enhanced migration and invasion, whereas HCRP1 upregulation in SMMC-7721 cells (with high invasion capacity) generated the opposite result. Interestingly, the morphology of HepG2 cells significantly changed from an epithelial to mesenchymal phenotype after HCRP1 knockdown. Moreover, we observed a decrease in the expression of epithelial cell markers E-cadherin and beta-catenin, and an increase in the expression of mesenchymal cell markers N-cadherin and vimentin. We also observed that the downregulation of HCRP1 induced epithelial-mesenchymal transition (EMT) through the transforming growth factor-beta pathway. Together, our findings define a novel function for HCRP1 from the perspective of EMT, which is closely associated with the migration and invasion of HCC cells.

关键词

CANCER; EXPRESSION; PROGNOSIS; PROTEIN

基本信息

所属机构:

归属医师: 徐嘉雯

UT：000398139500018

刊名:INTERNATIONAL JOURNAL OF ONCOLOGY

年，卷(期):2017年50卷4期

页码：1233-1240

DOI：10.3892/ijo.2017.3903

附件：

收录: SCIE