Correlation of cancer stem cell markers and immune cell markers in resected non-small cell lung cancer
作者
Huang, ZQ;Yu, HN;Zhang, JB;Jing, HY;Zhu, WQ;Li, XL;Kong, LL;Xing, LG;Yu, JM;Meng, XJ
作者单位
[Huang, Zhaoqin] Shandong Univ, Dept Radiol, Shandong Prov Hosp, Jinan, Shandong, Peoples R China.;-;[Yu, Haining; Zhu, Wanqi; Li, Xiaolin; Kong, Lingling; Xing, Ligang; Yu, Jinming; Meng, Xiangjiao] Shandong Univ, Dept Radiat Oncol, Shandong Canc Hosp, Shandong Acad Med Sci, 440 Jiyan Rd, Jinan 250117, Shandong, Peoples R China.;-;[Zhang, Jianbo] Shandong Univ, Dept Pathol, Shandong Canc Hosp, Shandong Acad Med Sci, Jinan, Shandong, Peoples R China.;-;[Jing, Haiyan] Shandong Univ, Dept Pathol, Shandong Prov Hosp, Jinan, Shandong, Peoples R China.
摘要
Background: Recent studies confirmed that immunotherapy showed prominent efficacy in non-small cell lung cancer (NSCLC). Cancer stem cells/cancer initiating cells are resistant to anticancer treatment. The purpose of the study was to analyze the correlation of cancer stem cells/cancer initiating cells and tumor-infiltrating immune cells in NSCLC.;-;Methods: CD133, octamer 4 (OCT-4), CD8, CD56, human leukocyte antigen (HLA) class I and programmed death ligand-1 (PD-L1) were assessed in 172 resected NSCLC samples. The staining was analyzed and scored by the pathologist who was blinded to the clinical pathological data of the patients.;-;Results: High CD8+ T cell infiltration was correlated significantly with squamous cell carcinoma histology (p=0.008). High PD-L1 expression (>= 10%) was associated with high tumor status (p=0.043). Pearson's correlation test showed that CD56+ cells were negatively correlated with CD133 expression (r=-0.361, p<0.001) and weakly correlated with negative OCT-4 expression (r=-0.180, p=0.018). There was a strong positive correlation between CD8 and HLA class I (r=0.573, p<0.001). In the survival analysis, high CD8+ T cell infiltration is an independent predictor of improved disease-free survival and overall survival. Patients with low CD133 expression and high CD56 expression had a longer overall survival than those with high CD133 expression and/ or low CD56 expression (p=0.013).;-;Conclusion: There is a negative correlation between CD56+ cells and cancer stem cell markers. This correlation may confirm the possibility that natural killer cells can target CD133+ cancer stem cells/cancer initiating cells in non-small cell lung cancer.
关键词
INITIATING CELLS; CD133 EXPRESSION; STAGE; INFILTRATION; DOCETAXEL; NIVOLUMAB; BIOLOGY; IMPACT; TUMORS; OCT4