Gamma-synuclein binds to AKT and promotes cancer cell survival and proliferation

作者

Ma, ZX;Niu, JY;Sun, EL;Rong, XD;Zhang, XX;Ju, YR

作者单位

[Ma, Zengxia; Sun, Erlian; Ju, Yuanrong] Shandong Univ, Shandong Prov Hosp, Dept Resp, Jinan 250021, Peoples R China.;-;[Ma, Zengxia; Rong, Xuedong; Zhang, Xianxin] Shandong Prov Chest Hosp, Dept Resp, Jinan 250013, Peoples R China.;-;[Niu, Jianyi] Weifang Med Univ, Dept Neurol, Yidu Cent Hosp, Qingzhou 262500, Peoples R China.

摘要

Hyperactivation of AKT plays a critical role in the survival and proliferation of cancer cells. However, the molecular mechanisms underlying AKT activation remain elusive. Here, we tested the effect of gamma-synuclein, a member of the synuclein family of proteins, on the activation of AKT. We show that the expression level of gamma-synuclein is increased in non-small cell lung cancer (NSCLC) tissues. gamma-Synuclein binds to the protein kinase domain of AKT and promotes its phosphorylation. Overexpression of gamma-synuclein in H157 cells enhances cell proliferation and protects the cells from staurosporine-induced cytotoxicity. Knockdown of gamma-synuclein attenuates AKT activation and cell proliferation induced by epidermal growth factor. The effect of gamma-synuclein is abolished when AKT is depleted. Thus, gamma-synuclein promotes cell survival and proliferation via activating AKT and may play a causal role in the pathogenesis of NSCLC.

关键词

LUNG-CANCER; MTOR PATHWAY; GENE; KINASE; EXPRESSION; IDENTIFICATION; METASTASIS; RESISTANCE; MUTATION; THERAPY

基本信息

所属机构:

归属医师: 鞠远荣孙尔莲

PMID：27655287

UT：000388799900061

刊名:TUMOR BIOLOGY

年，卷(期):2016年37卷11期

页码：14999-15005

DOI：10.1007/s13277-016-5371-9

附件：

收录: SCIE