miR-494 inhibits invasion and proliferation of gastric cancer by targeting IGF-1R

作者

Zhao, XQ;Liang, TJ;Fu, JW

作者单位

[Zhao, X. -Q.; Liang, T. -J.] Shandong Univ, Shandong Prov Hosp, Dept Digest Dis, Jinan, Shandong, Peoples R China.;-;[Fu, J. -W.] Jining 1 Peoples Hosp, Dept Gastrointestinal Surg, Jining, Shandong, Peoples R China.

摘要

OBJECTIVE: The aim of this study was to investigate the target gene of miR-494 and its roles in tumor growth of gastric cancer (GC).;-;PATIENTS AND METHODS: Expression of miR-494 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in gastric cancer tissues and cell lines. Then, luciferase reporter assay was used to elucidate whether insulin-like growth factor 1 receptor (IGF1R) is a target gene of miR-494. Finally, the roles and mechanism of miR-494 in the regulation of tumor invasion were further investigated.;-;RESULTS: Relative miR-494 level was found to be significantly lower in patients with GC than healthy controls (p < 0.01). Over-expression of miR-494 could inhibit gastric cancer cell proliferation, migration, and invasion in vitro. Furthermore, we demonstrated that miR-494 binds to the 3'-untranslated region (UTR) of IGF1R and inhibits the expression of the IGF1R protein.;-;CONCLUSIONS: Our data showed that miR-494 acted as a tumor suppressor in GC.

关键词

CELL-PROLIFERATION; HEPATOCELLULAR-CARCINOMA; GROWTH; SENSITIVITY; PROGRESSION; SUPPRESSES; MICRORNAS; MIGRATION; RECEPTOR; PTEN

基本信息

所属机构:

归属医师: 梁铁军

PMID：27735036

UT：000386564200014

刊名:EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES

年，卷(期):2016年20卷18期

页码：3818-3824

附件：

收录: SCIE