KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES AND THEIR HLA-C LIGANDS IN HASHIMOTO THYROIDITIS IN A CHINESE POPULATION
作者
Li, JT;Guo, C;Li, ML;Wei, YQ;Hou, YF;Jiao, YL;Zhao, YR;Sun, H;Xu, J;Cao, MF;Feng, L;Yu, GN;Gao, L;Liu, YQ;Zhang, BC;Zhao, JJ;Zhang, HQ
作者单位
[Li, Jian-ting; Guo, Cheng; Li, Ming-long; Xu, Jin; Cao, Ming-feng; Feng, Li; Yu, Gui-na; Gao, Ling; Zhao, Jia-jun; Zhang, Hai-qing] Shandong Univ, Shandong Prov Hosp, Dept Endocrinol, Jinan, Peoples R China.;-;[Li, Jian-ting] Shandong Univ, Jinan Cent Hosp, Dept Endocrinol, Jinan, Peoples R China.;-;[Guo, Cheng] Jining Med Univ, Affiliated Hosp, Jining, Peoples R China.;-;[Wei, Yong-qing] Shandong Univ, Jinan Cent Hosp, Dept Gynecol & Obstet, Jinan, Peoples R China.;-;[Hou, Yan-feng] Shandong Univ, Qianfoshan Hosp Shandong Prov, Dept Rheumatol, Jinan, Peoples R China.;-;[Jiao, Yu-lian; Zhao, Yue-ran] Shandong Univ, Shandong Prov Hosp, Sci Ctr, Jinan, Peoples R China.;-;[Sun, Hui; Liu, Yi-qing; Zhang, Bing-chang] Shandong Univ, Shandong Prov Hosp, Dept Lab Med, Jinan, Peoples R China.;-;[Gao, Ling; Zhao, Jia-jun; Zhang, Hai-qing] Shandong Clin Med Ctr Endocrinol & Metab, Jinan, Peoples R China.;-;[Gao, Ling; Zhao, Jia-jun; Zhang, Hai-qing] Shandong Acad Clin Med, Inst Endocrinol & Metab, Jinan, Peoples R China.
摘要
Objective: Natural killer (NK) cells serve as primary immune surveillance and are partially regulated by combinations of killer immunoglobulin-like receptors (KIR) and their human leukocyte antigen-C (HLA-C) ligands. Alterations in NK cell activity have been associated with Hashimoto thyroiditis (HT). The aim of this study was to determine whether certain KIR/HLA-C genotype combinations play a role in HT pathogenesis.;-;Methods: The present study enrolled 107 unrelated HT patients and 108 random healthy individuals in a case-control study. Blood was collected for DNA extraction; typing of KIR genes and HLA-C alleles was performed by polymerase chain reaction with sequence specific primers (PCR-SSP), followed by electrophoresis on agarose gels.;-;Results: Among a panel of KIR2D/HLA-C genotype combinations, the frequency of KIR2DS2/HLA-C1 was significantly increased in HT patients compared to controls (33.64% vs. 12.96%, P<.001). To further analyze the precise genotype, we investigated inhibitory or activating KIR/HLA-C gene pairs when their corresponding activating or inhibitory KIR genes were absent in the 2 groups. Only the frequency of KIR2DS2(-)2DL2/3(+)HLA-C1(+) was significantly decreased in HT patients compared to controls (48.60% vs. 70.37%, P = .001).;-;Conclusion: Our data suggest that KIR2DS2/HLA-C1 may correlate with HT pathogenesis. On the contrary, the predominance of KIR2DL2/3/HLA-C1 in the absence of KIR2DS2 suggests a potential inhibitory role in HT pathogenesis. In conclusion, our findings may further elucidate the mechanisms underlying the pathogenesis of HT and other autoimmune diseases.
关键词
CLASS-I MOLECULES; AUTOREACTIVE T-CELLS; ACTIVATING KIR GENES; LEUKOCYTE ANTIGEN-C; MHC CLASS-I; INHIBITORY RECEPTORS; GRAVES-DISEASE; MATERNAL KIR; LYMPHOCYTES; ARTHRITIS
基本信息
