MiR-130b inhibits proliferation and induces apoptosis of gastric cancer cells via CYLD

作者

Sun, BY;Li, L;Ma, WD;Wang, SK;Huang, CJ

作者单位

[Sun, Baoyou; Li, Lei; Ma, Wendong; Wang, Shikang] Shandong Univ, Shandong Prov Hosp, Jinan 250014, Peoples R China.;-;[Huang, Chunjin] Fudan Univ, Huadong Hosp, Dept Gen Surg, 221 Yananxi Rd, Shanghai 200040, Peoples R China.

摘要

A role of microRNA-130b (miR-130b) in the carcinogenesis of gastric cancer remains undetermined. In this study, we studied the effects and mechanism of miR-130b to the gastric cell proliferation and apoptosis. We found that the levels of miR-130b significantly up-regulated in gastric cancer tissue, compared to the paired adjacent non-tumor gastric tissue. The miR-130b levels in gastric cancer cell lines were significantly higher than those in control normal gastric tissues. Transfection with the miR-130b mimic enhanced the cell proliferation and suppressed cell apoptosis in gastric cancer cells, while transfection with the anti-sense of miR-130b (anti-miR-130b) suppressed cell proliferation and induced cell apoptosis in gastric cancer cells. Bioinformatics analyses showed that cylindromatosis gene (CYLD) was a potential target gene of miR-130b. The luciferase activity assay and western blot verified that miR-130b targeted CYLD messenger RNA (mRNA) to modulate its protein levels. Together, our study suggests that aberrantly expressed miR-130b may regulate cell apoptosis and proliferation of human gastric cancer cells via CYLD, which appears to be a promising therapeutic target for gastric cancer.

关键词

DOWN-REGULATION; DEUBIQUITINASE CYLD; BREAST-CANCER; METASTASIS; GROWTH; OSTEOSARCOMA; MMP7; PHOSPHORYLATION; INFLAMMATION; ACTIVATION

基本信息

所属机构:急救中心

归属医师: 李磊孙宝友王世康

PMID：26711782

UT：000376464700098

刊名:TUMOR BIOLOGY

年，卷(期):2016年37卷6期

页码：7981-7987

DOI：10.1007/s13277-015-4632-3

附件：

收录: SCIE