Development of a Novel, Anti-idiotypic Monoclonal Anti-prolactin Antibody That Mimics the Physiological Functions of Prolactin

作者

Wang, M;Zhang, DC;Wang, ST;Li, ML

作者单位

[Wang, Meng; Wang, Shen-Tian] Yidu Cent Hosp Weifang, Endocrine Dept, Weifang 262500, Peoples R China.;-;[Zhang, Dian-Cai] Yidu Cent Hosp Weifang, Dept Dermatol, Weifang 262500, Peoples R China.;-;[Li, Ming-Long] China Shandong Prov Hosp, Jinan 250000, Peoples R China.

摘要

In this work, we prepared a panel of monoclonal anti-idiotypic antibodies to ovine prolactin (oPRL) by the hybridoma technique. Among these antibodies, one anti-idotypic antibody (designated B7) was chosen for further characterization by a series of experiments. We first demonstrated that B7 behaved as a typical Ab2 beta based on a series of enzyme-linked immunosorbent assays. Subsequently, the results of a competitive receptor-binding assay confirmed that B7 could specifically bind to the prolactin receptor (PRLR) expressed on target cells. Finally, we examined its biological activities in CHO-PRLR and Nb2 cells and observed that B7 could activate Janus kinase 2-signal transducer and activator of transcription signalling in CHO-PRLR and Nb2 cells and induce BaF3 proliferation. The present study suggests that i) B7 can serve as a PRLR agonist or PRL mimic and has potential applications in regulating mammary gland development, milk production and maintenance of lactation in domestic animals and ii) B7 may be a biological reagent that can be used to explore the mechanism of PRLR-mediated intracellular signalling.

关键词

MAMMARY-GLAND; GROWTH-HORMONE; PRL RECEPTOR; PERFORMANCE; ACTIVATION; LACTATION

基本信息

所属机构:

归属医师: 李明龙

PMID：26949959

UT：000371459100015

刊名:ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES

年，卷(期):2016年29卷4期

页码：571-579

DOI：10.5713/ajas.15.0541

附件： pdf

收录: SCIE