Effects of PARP-1 inhibitor and ERK inhibitor on epithelial mesenchymal transitions of the ovarian cancer SKOV3 cells

作者

Su, S;Lin, XY;Ding, N;Zhang, H;Zhang, QH;Ding, YM;Hou, XM;Tian, YJ

作者单位

[Su, Shan; Lin, Xueyan; Hou, Xiaoman; Tian, Yongjie] Shandong Univ, Dept Obstet & Gynecol, Prov Hosp, Jinan, Shangdong, Peoples R China.;-;[Su, Shan; Ding, Ning; Zhang, Hong; Zhang, Qinghua; Ding, Yumei] Cent Hosp Zibo, Dept Gynecol, Zibo, Shangdong, Peoples R China.

摘要

Background: To assess the effects of the poly (ADP-ribose) polymerase-1 (PARP-1) inhibitor PJ34 and ERK1/2 inhibitor U0126 on the proliferation and epithelial mesenchymal transitions (EMT) of cisplatin resistant ovarian cancer SKOV-3 cells.;-;Methods: Proliferation of SKOV-3 cells was evaluated using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide assay with PJ34 and U0126 treatment. Expression changes of E-cadherin and vimentin with PJ34 and U0126 treatment was examined using Western blot and quantitative PCR. In addition, invasion assay was performed in cells treated with PJ34 and U0126.;-;Results: PJ34 and U0126 inhibited proliferation of SKOV-3 cells in a time dependent manner. PJ34 and U0126 suppressed the expression of vimentin and enhanced the expression of E-cadherin. PJ34 and U0126 reduced cell invasion. The inhibitory effects of PJ34 and U0126 were stronger than PJ34 alone. PJ34 inhibited the proliferation and invasion of SKOV-3 cells which can be enhanced by ERK1/2 inhibitor U0126.;-;Conclusions: These inhibitory effects are partially due to PARP-1 and ERK1/2 mediated attenuation of EMT activity. (C) 2016 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Sp. z o.o. All rights reserved.

关键词

KAPPA-B; POLY(ADP-RIBOSE) POLYMERASE-1; ACTIVATION; BINDING; KINASE; C-13-ASTERISK-CELLS; PROLIFERATION; DOMAINS

基本信息

所属机构:

归属医师: 田永杰林雪艳

PMID：27668317

UT：000388432100018

刊名:PHARMACOLOGICAL REPORTS

年，卷(期):2016年68卷6期

页码：1225-1229

DOI：10.1016/j.pharep.2016.08.001

附件：

收录: SCIE