Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer

作者

Gao, H;Liu, YJ;Li, K;Wu, TH;Peng, JJ;Jing, FB

作者单位

[Gao, Hui] Qingdao Univ, Coll Med, Qingdao 266071, Shandong, Peoples R China.;-;[Liu, Yongji] Qingdao Hiser Med Ctr, Qingdao 266000, Shandong, Peoples R China.;-;[Li, Kan] Shandong Univ, Shandong Prov Hosp, Jinan 250021, Shandong, Peoples R China.;-;[Wu, Tianhui] Qingdao 5th Peoples Hosp, Qingdao 266000, Shandong, Peoples R China.;-;[Peng, Jianjun] Chongqing Normal Univ, Coll Life Sci, Chongqing 401331, Peoples R China.;-;[Jing, Fanbo] Qingdao Univ, Affiliated Hosp, Qingdao 266003, Shandong, Peoples R China.

摘要

Acute myeloid leukemia (AML) represents a heterogeneous group of hematological neoplasms with marked heterogeneity in response to both standard therapy and survival. Hispidulin, a flavonoid compound that is anactive ingredient in the traditional Chinese medicinal herb Salvia plebeia R. Br, has recently been reported to have anantitumor effect against solid tumors in vitro and in vivo. The aim of the present study was to investigate the effects of hispidulin on the human leukemia cell line in vitro and the underlying mechanisms of its actions on these cells. Our results showed that hispidulin inhibits AML cell proliferation in a dose-and time-dependent manner, and induces cell apoptosis throughan intrinsic mitochondrial pathway. Our results also revealed that hispidulin treatment significantly inhibits extracellular matrix metalloproteinase inducer (EMMPRIN) expression in both tested AML cell lines in a dose-dependent manner, and that the overexpression of EMMPRIN protein markedly attenuates hispidulin-induced cell apoptosis. Furthermore, our results strongly indicated that the modulating effect of hispidulin on EMMPRIN is correlated with its inhibitory effect on both the Akt and STAT3 signaling pathways.

关键词

CANCER-CELLS; SIGNALING PATHWAY; RECOGNITION MOLECULE; PANCREATIC-CANCER; ACTIVATING AMPK; DOWN-REGULATION; IN-VITRO; CD147; GROWTH; ANGIOGENESIS

基本信息

所属机构:

归属医师: 李衎

PMID：27158398

UT：000373944000075

刊名:AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

年，卷(期):2016年8卷2期

页码：1115-1132

附件： pdf

收录: SCIE