Pharmacokinetic study of isatin in dog plasma by liquid chromatography tandem mass spectrometry
作者
Ren, A;Wang, Q;Fang, Z;Gao, M;Wang, H;Zhang, J;Xu, W;Yue, W;Yin, L;Liu, Z;Li, X;Ding, B
作者单位
[Ren, A.; Wang, Q.; Fang, Z.; Gao, M.; Zhang, J.; Ding, B.] Shandong Univ, Shandong Prov Hosp, Jinan 250100, Peoples R China.;-;[Xu, W.; Yue, W.; Yin, L.; Liu, Z.; Li, X.] Qingdao Univ, Affiliated Hosp, Qingdao 266071, Peoples R China.;-;[Wang, H.] Hosp Luzhong Min Co Ltd, Laiwu, Peoples R China.
摘要
Aim. A sensitive and selective method was developed and validated to study the pharmacokinetics of isatin.;-;Methods. The blood samples were pretreated by protein precipitation method using methanol. Quetiapine was used as an internal standard. After pretreatment, the samples were assayed by LC/MS/NIS method and the pharmacokinetic parameters were calculated by WinNonlin 5.2 using non-compartment model. The separation was performed on a Venusil XBP PH column (5 mu m, 2.0x100 mm) with an isocratic mobile phase consisted of methanol-water (containing 50 mM ammonium formate) (65:35, v/v) at a flow rate of 0.3 mL/min. The Agilent G6410B triple quadrupole LC/MS system was operated under the multiple reactions monitoring mode (MRM) using the electrospray ionization technique in positive mode.;-;Results. The lower limits of quantification (LLOQ) of the analyte of the method was 10 ng/mL. The method was linear with correlation coefficient >0.995. The intraday and inter day accuracy and precision of the assay were acceptable.;-;Conclusion. This method has been applied successfully to a pharmacokinetic study involving the oral and intravenous administration of isatin to beagle dogs.
关键词
URINE; INHIBITOR; BRAIN
基本信息
