Immunotherapy for Lewis lung carcinoma utilizing dendritic cells infected with CK19 gene recombinant adenoviral vectors

作者

Sun, QF;Zhao, XN;Peng, CL;Hao, YT;Zhao, YP;Jiang, N;Xue, H;Guo, JZ;Yun, CH;Cong, B;Zhao, XG

作者单位

[Sun, Q. F.; Peng, C. L.; Hao, Y. T.; Zhao, Y. P.; Jiang, N.; Xue, H.; Guo, J. Z.; Yun, C. H.; Cong, B.; Zhao, X. G.] Shandong Univ, Hosp 2, Dept Thorac Surg, Jinan 250033, Shandong, Peoples R China.;-;[Zhao, X. N.] Shandong Univ, Prov Hosp, Dept Comprehens Hlth 2, Jinan 250033, Shandong, Peoples R China.

摘要

Dendritic cells (DCs) as 'professional' antigen-presenting cells (APCs) initiate and regulate immune responses to various antigens. DC-based vaccines have become a promising modality in cancer immunotherapy. Cytokeratin 19 (CK19) protein is expressed at high levels in lung cancer and many other tumor cells, suggesting CK19 as a potential tumor-specific target for cancer immune therapy. We constructed a recombinant adenoviral vector containing the CK19 gene (rAd-CK19). DCs transfected with rAd-CK19 were used to vaccinate C57BL/6 mice bearing xenografts derived from Lewis lung carcinoma (LLC) cells. The transfected DCs gave rise to potent CK19-specific cytotoxic T lymphocytes (CTLs) capable of lysing LLC cells. Mice immunized with the rAd-CK19-DCs exhibited significantly attenuated tumor growth (including tumor volume and weight) when compared to the tumor growth of mice immunized with rAd-c DCs or DCs during the 24-day observation period (P<0.05). The results revealed that the mice vaccinated with the rAd-CK19-DCs exhibited a potent protective and therapeutic antitumor immunity to LLC cells in the subcutaneous model along with an inhibitive effect on tumor growth compared to the mice vaccinated with the rAd-c DCs or DCs alone. The present study proposes a meaningful mode of action utilizing rAd-CK19 DCs in lung cancer immunotherapy.

关键词

POLYMERASE-CHAIN-REACTION; MESSENGER-RNA EXPRESSION; CYTOTOXIC T-LYMPHOCYTES; GROWTH-FACTOR RECEPTOR; IN-VITRO; ANTITUMOR IMMUNITY; TUMOR VACCINES; BREAST-CANCER; ANTIGENS; INDUCTION

基本信息

所属机构:

归属医师: 赵雪柠

PMID：26323510

UT：000362857300010

刊名:ONCOLOGY REPORTS

年，卷(期):2015年34卷5期

页码：2289-2295

DOI：10.3892/or.2015.4231

附件： pdf

收录: SCIE