Adenovirus siMDM2 and NDRG2 Gene Therapy Inhibits Cell Proliferation and Induces Apoptosis of Squamous Cell Carcinoma

作者

Wang, SZ;Chen, N;Dong, N;Lu, LH;Liu, LQ;Zhang, L

作者单位

[Wang, Shouzhong; Chen, Nan; Zhang, Li] Shandong Univ, Shandong Prov Hosp, Dept Dermatol, Jinan 250021, Peoples R China.;-;[Wang, Shouzhong; Dong, Na; Lu, Leihong; Liu, Liqian] Linyi Peoples Hosp, Dept Dermatol, Linyi 276003, Peoples R China.

摘要

Squamous cell carcinoma (SCC) is one of the most common skin cancers. In the present study, we explored the effects of depletion of murine double minute gene 2 (MDM2) together with overexpression of N-myc downstream-regulated gene 2 (NDRG2) on cutaneous SCC. In order to achieve high efficiency of gene knockdown and overexpression in SCC-13 cells, recombinant adenovirus carrying siMDM2 and NDRG2 expression construct was produced. We found Ad-siMDM2, Ad-NDRG2, and Ad-siMDM2-NDRG2 infections inhibit the growth of SCC-13 cells in vitro, and Ad-siMDM2-NDRG2 infection has the highest inhibitory effect. Subcutaneous injections of Ad-siMDM2, Ad-NDRG2, and Ad-siMDM2-NDRG2 into SCC-13 xenograft nude mice resulted in the reduction of tumor volume. Moreover, we found that apoptosis protein caspase 3 was up-regulated in the Ad-siMDM2-, Ad-NDRG2-, and Ad-siMDM2-NDRG2-treated groups. Our data indicate that the adenovirus-mediated MDM2 silencing and NDRG2 overexpression can synergistically inhibit local cancer cell proliferation, induce apoptosis, and further prevent metastases of SCC. Our study provides a promising method that can be further developed as a new therapeutic approach against SCC.

关键词

POOR-PROGNOSIS; 2 EXPRESSION; CANCER; ADENOCARCINOMA; COMPLEX; TARGET; MDM2

基本信息

所属机构:

归属医师: 张莉陈楠

PMID：27352346

UT：000362009900038

刊名:CELL BIOCHEMISTRY AND BIOPHYSICS

年，卷(期):2015年73卷2期

页码：513-518

DOI：10.1007/s12013-015-0691-8

附件：

收录: SCIE