Expression of Surfactant Protein-A during LPS-Induced Otitis Media with Effusion in Mice
作者
Li, L;Guo, XR;Olszewski, E;Fan, ZM;Ai, Y;Han, Y;Xu, L;Li, JF;Wang, HB
作者单位
[Li, Li; Guo, Xiangrui; Fan, Zhaomin; Ai, Yu; Han, Yuechen; Xu, Lei; Li, Jianfeng; Wang, Haibo] Shandong Univ, Prov Hosp, Dept Otolaryngol Head & Neck Surg, Jinan 250021, Peoples R China.;-;[Li, Li; Guo, Xiangrui; Fan, Zhaomin; Ai, Yu; Han, Yuechen; Xu, Lei; Li, Jianfeng; Wang, Haibo] Shandong Inst Otolaryngol, Jinan, Peoples R China.;-;[Li, Li; Guo, Xiangrui; Fan, Zhaomin; Ai, Yu; Han, Yuechen; Xu, Lei; Li, Jianfeng; Wang, Haibo] Shandong Prov Key Lab Otol, Jinan, Peoples R China.;-;[Olszewski, Emily] Univ Wisconsin, Sch Med & Publ Hlth, Dept Surg, Div Otolaryngol Head & Neck Surg, Madison, WI USA.
摘要
Objective The objective of this study was to investigate the expression and role of surfactant protein (SP) in the middle ear throughout lipopolysaccharide (LPS)-induced otitis media with effusion (OME).;-;Study Design Randomized case-controlled animal model.;-;Setting Shandong University, Jinan, China.;-;Subjects and Methods SP expression was monitored using reverse transcription polymerase chain reaction (PCR) in normal mice (n = 5). Two groups, control phosphate-buffered saline-injected mice (n = 5) and LPS-injected mice (n = 5), were euthanized 5 days following injection. RNA was extracted for reverse transcription PCR and real-time PCR, and temporal bone samples were used for hematoxylin and eosin staining. LPS was injected into mice, and 5 mice per test were euthanized at 0, 12, 24, 48, 72, and 96 hours following injection. For mRNA expression quantification, reverse transcription PCR and real-time PCR were performed, and proinflammatory cytokine levels were measured by enzyme-linked immunosorbent assay.;-;Results SP-A and SP-D expression was detected in normal murine Eustachian tubes. SP-A expression was up-regulated after LPS-induced OME (P = .01). At various time points after LPS injection, concentrations of proinflammatory cytokines (tumor necrosis factor-[TNF-], interleukin (IL)-1, and IL-6) in the middle ear increased significantly (P < .05) and correlated with changes in SP-A expression.;-;Conclusion SP-A and SP-D exist in the murine middle ear. The expression of SP-A and TNF-, IL-1, and IL-6 was up-regulated in the middle ear of the LPS-induced OME animal model.
关键词
PULMONARY SURFACTANT; EUSTACHIAN-TUBE; TNF-ALPHA; INFLAMMATORY RESPONSE; UP-REGULATION; LUNG; CYTOKINES; PHAGOCYTOSIS; MACROPHAGES; EPITHELIUM
基本信息
