Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line
作者
Zhang, X;Jiang, SJ;Shang, B;Jiang, HJ
作者单位
[Zhang, Xuan; Jiang, Hong-Juan] Shandong Univ, Shandong Prov Hosp, Dept Resp Healthcare Med, Jinan 250021, Shandong, Peoples R China.;-;[Jiang, Shu-Juan] Shandong Univ, Shandong Prov Hosp, Dept Resp Pulm Med, Jinan 250021, Shandong, Peoples R China.;-;[Shang, Bin] Shandong Univ, Shandong Prov Hosp, Dept Thorac Surg Med, Jinan 250021, Shandong, Peoples R China.
摘要
BackgroundHistone deacetylase (HDAC) inhibitors combined with other drugs for the treatment of malignant tumors are used more and more widely. In this study, we investigated the effect of trichostatin A (TSA), a HDAC inhibitor, in combination with cisplatin, a cytotoxic chemotherapy agent, on the apoptosis of lung cancer A549 cells.;-;MethodsA549 cells were treated with TSA alone, cisplatin alone or the two drugs combined. Cell viability and apoptosis were evaluated using a light microscope, methyl thiazolyl tetrazolium (MTT) (3-[4, 5-dimethylthiazol-2-yl] -2, 5-diphenyltetrazolium bromide) assay and Hochst33258 staining. Moreover, Western blot analysis was employed to examine the alterations of apoptosis protein: cellular Fas-associated death domain-like interleukin-1 converting enzyme inhibitory protein (cFLIP) and caspase-8 in A549 cells in response to the different exogenous stimuli.;-;ResultsCompared with single-agent treatment, the co-treatment of A549 cells with TSA and cisplatin synergistically inhibited cell proliferation, induced apoptosis, and increased the inhibition rate. Treatment with TSA and cisplatin led to a significant decrease of cFLIP expression. Furthermore, the treatment of A549 cells with TSA and cisplatin resulted in a significant decrease of pro-caspase-8 and a significant increase of caspase-8.;-;ConclusionsSynergistic anti-tumor effects are observed between cisplatin and TSA in lung cancer cells. The combination of TSA with cisplatin may be a more effective method in human lung cancer treatment.
关键词
CANCER-CELLS; ANTITUMOR-ACTIVITY; LUNG-CANCER; DOCETAXEL; PROTEIN; DEATH; INDUCTION; DISEASE; FUTURE; GENES
基本信息
