Prestin at year 14: Progress and prospect

作者

He, DZZ;Lovas, S;Ai, Y;Li, Y;Beisel, KW

作者单位

[He, David Z. Z.; Lovas, Sandor; Li, Yi; Beisel, Kirk W.] Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA.;-;[He, David Z. Z.] Ningbo Univ, Sch Med, Ctr Neurosci, Ningbo 315211, Zhejiang, Peoples R China.;-;[Ai, Yu] Shandong Prov Hosp, Dept Otolaryngol, Jinan 250021, Peoples R China.;-;[Li, Yi] Beijing Tongren Hosp, Dept Otolaryngol, Beijing 100730, Peoples R China.

摘要

Prestin, the motor protein of cochlear outer hair cells, was identified 14 years ago. Prestin-based outer hair cell motility is responsible for the exquisite sensitivity and frequency selectivity seen in the mammalian cochlea. Prestin is the 5th member of an eleven-member membrane transporter superfamily of SLC26A proteins. Unlike its paralogs, which are capable of transporting anions across the cell membrane, prestin primarily functions as a motor protein with unique capability of performing direct and reciprocal electromechanical conversion on microsecond time scale. Significant progress in the understanding of its structure and the molecular mechanism has been made in recent years using electrophysiological, biochemical, comparative genomics, structural bioinformatics, molecular dynamics simulation, site-directed mutagenesis and domain-swapping techniques. This article reviews recent advances of the structural and functional properties of prestin with focus on the areas that are critical but still controversial in understanding the molecular mechanism of how prestin works: The structural domains for voltage sensing and interaction with anions and for conformational change. Future research directions and potential application of prestin are also discussed. This article is part of a Special Issue entitled . (C) 2013 Elsevier B.V. All rights reserved.

关键词

OUTER HAIR-CELLS; MOTOR PROTEIN PRESTIN; GUINEA-PIG COCHLEA; MULTIFUNCTIONAL ANION-EXCHANGERS; CYSTEINE MUTAGENESIS REVEALS; RESONANCE ENERGY-TRANSFER; ATOMIC-FORCE MICROSCOPY; PLASMA-MEMBRANE; VOLTAGE-SENSOR; SEQUENCE EVOLUTION

基本信息

所属机构:

归属医师: 艾毓

PMID：24361298

UT：000336355300004

刊名:HEARING RESEARCH

年，卷(期):2014年311卷

页码：25-35

DOI：10.1016/j.heares.2013.12.002

附件： pdf

收录: SCIE