Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro

作者

Yu, XM;Li, CW;Chao, SS;Li, YY;Yan, Y;Zhao, XN;Yu, FG;Liu, J;Shen, L;Pan, XL;Shi, L;Wang, DY

作者单位

[Yu, Xue Min; Zhao, Xue Ning; Pan, Xin Liang; Shi, Li] Shandong Univ, Qilu Hosp, Dept Otolaryngol, Jinan, Peoples R China.;-;[Yu, Xue Min; Li, Chun Wei; Chao, Siew Shuen; Li, Ying Ying; Yan, Yan; Yu, Feng Gang; Liu, Jing; Wang, De Yun] Natl Univ Singapore, Natl Univ Hlth Syst, Dept Otolaryngol, Singapore, Singapore.;-;[Zhao, Xue Ning] Shandong Univ, Shandong Prov Hosp, Dept Gen Hlth Care 2, Jinan, Peoples R China.;-;[Shen, Liang] Shandong Univ, Shandong Prov Hosp, Biostat Unit, Jinan, Peoples R China.

摘要

Basal cells in nasal epithelium have stemness/progenitor characters and play essential roles in the epithelial remodeling in nasal polyps (NP). We investigate whether the human nasal epithelial stem/progenitor cells (hNESPCs) from patients with NP are inherently distinct from those obtained from healthy controls. Epithelial basal cells were isolated and cultured for four passages from NP tissues and control nasal mucosa. hNESPCs from controls were stained positively with stem cell marker p63 and KRT5 and presented a consistent high Ki67 expression level over four passages. In contrast, hNESPCs from NP patients showed: i). a reduced growth and proliferation rate at each passage by evaluating colony-forming efficiency and doubling time; ii). a lower percentage of Ki67(+) cells among p63(+) cells in the colonies in late passages, which was also confirmed by immunostaining in the NP tissues. Thus reduced growth/proliferation dynamics in hNESPCs from NP could be an important pathological phenomenon in NP development.

关键词

CHRONIC RHINOSINUSITIS; UPPER AIRWAYS; UP-REGULATION; STEM-CELLS; EXPRESSION; DISEASE; REPAIR; TIME

基本信息

所属机构:

归属医师: 赵雪柠沈亮

PMID：24714674

UT：000333962800002

刊名:SCIENTIFIC REPORTS

年，卷(期):2014年4卷

DOI：10.1038/srep04619

附件： pdf

收录: SCIE