Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem-like cell properties by high aldehyde dehydrogenase activity

作者

Yu, FG;Sim, ACN;Li, CW;Li, YY;Zhao, XN;Wang, DY;Loh, KS

作者单位

[Yu, Fenggang; Li, Chunwei; Li, Yingying; Wang, De-Yun; Loh, Kwok Seng] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Otolaryngol, Singapore 119228, Singapore.;-;[Sim, Adrian Chong Nyi] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, Singapore 119228, Singapore.;-;[Zhao, Xuening] Shandong Univ, Prov Hosp, Dept Gen Hlth Care 2, Jinan 250100, Peoples R China.;-;[Loh, Kwok Seng] Natl Univ Hlth Syst, Natl Canc Inst Singapore, Head & Neck Tumor Grp, Singapore, Singapore.

摘要

Objectives/Hypothesis Cancer stem cells have been reported as a new therapeutic target in many cancers, but their existence in nasopharyngeal carcinoma (NPC) is largely unknown. This study was conducted to determine cancer stem-like cells in NPC cell line.;-;Study Design Basic science experimental study.;-;Methods Aldehyde dehydrogenase (ALDH) activity, a putative functional marker for cancer stem cells, was assessed in Epstein-Barr virus-associated NPC cell line C666-1 cells. The ability of cells with high and low ALDH activity to proliferate, resist therapy, and initiate tumor formation was compared.;-;Results Enrichment of cancer stem-like cells (with high ALDH activity) in C666-1 was associated with a significantly greater ability to proliferate, be clonogenic, resist chemotherapy drugs and radiation, reconstitute a heterogeneous population, and express pluripotent markers. Furthermore, subcutaneous injection of these cells into immunodeficient nude mice resulted in a tendency of tumor formation at a higher rate as compared to cells with low ALDH activity.;-;Conclusions These results provide evidence for the existence of cancer stem-like cells in the NPC cell line C666-1 cells.

关键词

EPSTEIN-BARR-VIRUS; ACUTE MYELOID-LEUKEMIA; BREAST CANCERS; 1 EXPRESSION; MARKER; CHEMOTHERAPY; METASTASIS; INHIBITION; RESISTANCE; PREDICTOR

基本信息

所属机构:

归属医师: 赵雪柠

PMID：23616401

UT：000322004400016

刊名:LARYNGOSCOPE

年，卷(期):2013年123卷8期

页码：1903-1911

DOI：10.1002/lary.24003

附件：

收录: SCIE