Alteration of discoidin domain receptor-2 expression: possible role in peroxynitrite-induced apoptosis in human cerebral vascular smooth muscle cells
作者
Li, L;Yue, ZY;Wan, XJ;Zhang, GD;Song, SS;Bai, XH;Jiao, YL;Ju, YR;Li, JF
作者单位
[Ju, Yuanrong] Shandong Univ, Prov Hosp, Intens Care Unit, Jinan 250021, Peoples R China.;-;[Li, Lin; Yue, Zhiyong; Wan, Xiaojuan; Zhang, Guodong; Song, Shasha; Bai, Xiaohui; Li, Jianfeng] Shandong Univ, Prov Hosp, Dept Otolaryngol Head & Neck Surg, Jinan 250021, Peoples R China.;-;[Jiao, Yulian; Li, Jianfeng] Shandong Univ, Prov Hosp, Cent Lab, Jinan 250021, Peoples R China.
摘要
Peroxynitrite(ONOO-), a product of nitric oxide and superoxide anion interaction, is a powerful and versatile oxidant. In this work, the human cerebral vascular smooth muscle cells (HCVSMCs) were cultured in vitro and subjected to different concentrations of ONOO-. Cell viability and morphological changes were measured by methylthiazoletetrazolium (MTT) assay and Ho33342/PI double staining respectively. The apoptotic rate was assessed by flow cytometry. The alterations in the expression of DDR2 at both mRNA and protein levels were examined by real-time PCR and Western-blot simultaneously. Direct exposure of HCVSMCs to ONOO- was able to inhibit the cell proliferation, which was further revealed via the apoptotic pathway. ONOO- exerted a dual-effect on DDR2 expression at both mRNA and protein levels that depended on the concentrations of ONOO-. Our results demonstrate for the first time that DDR2 gene may play an important role in ONOO--induced apoptosis in HCVSMCs.
关键词
NITRIC-OXIDE; SUPEROXIDE; COLLAGEN; DISEASES; ACTIVATION; OXYGEN
基本信息
