Peroxynitrite-induced apoptosis in human brain vascular smooth muscle cells is associated with induction of PDCD4 gene expression.
作者
Li, Y;Li, L;Jiao, YL;Xu, CY;Zhang, GD;Liu, WW;Yue, ZY;Li, JF
作者单位
[Li, Yang; Xu, Chengyan; Li, Jianfeng] Shandong Univ, Ctr Reprod Med, Shandong Prov Hosp, Jinan 250021, Peoples R China.;-;[Li, Lin; Zhang, Guodong; Liu, Wenwen; Yue, Zhiyong; Li, Jianfeng] Shandong Univ, Dept Otolaryngol Head & Neck Surg, Prov Hosp, Jinan 250021, Peoples R China.;-;[Li, Yang; Jiao, Yulian; Li, Jianfeng] Shandong Univ, Cent Lab, Prov Hosp, Jinan 250021, Peoples R China.
摘要
The present study investigated whether peroxynitrite (ONOO-)-elicited cytotoxicity on human brain vessel smooth muscle cells (HBVSMCs) was related to alteration in programmed cell death 4 (PDCD4) gene expression. Cell viability, morphological changes and apoptosis were determined by_methylthiazoletetrazolium (MTT) assay, acridine orange staining and flow cytometry, respectively. Expressions of PDCD4 at both mRNA and protein levels were examined by RT-PCR and Western blot respectively. Protein expressions of caspase-3, Bcl-2 and Bax were detected by Western an increased apoptotic rate of HBVSMCs after treatment with ONOO-. ONOO- was able to upregulate mRNA and protein expressions of the PDCD4 gene. Moreover, protein expressions of caspase-3 and Bax were significantly increased, whereas the expression of Bcl-2 was markedly decreased in response to exposure of ONOO-. This study demonstrates, for the first time, that the PDCD4 gene is involved in ONOO- induced apoptosis in HBVSMCs.
关键词
NITRIC-OXIDE
基本信息
