Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
作者
Song, SS;He, MQ;Li, L;Zhang, GD;Liu, WW;Bai, XH;Wang, HB;Yue, ZY;Li, JF
作者单位
[Song, Shasha; He, Mingqiang; Li, Lin; Wang, Haibo; Yue, Zhiyong; Li, Jianfeng] Shandong Univ, Prov Hosp, Dept Otolaryngol Head & Neck Surg, Jinan 250021, Peoples R China.;-;[Song, Shasha; Zhang, Guodong; Liu, Wenwen; Bai, Xiaohui; Wang, Haibo; Li, Jianfeng] Shandong Univ, Prov Hosp, Inst Eye & Otolaryngol, Jinan 250021, Peoples R China.
摘要
Peroxynitrite (ONOO-) is a highly reactive species that attacks a range of biological targets. The present study was designed to investigate the effect of ONOO- on FaDu cells, a human hypopharyngeal cancer cell line, with special attention given to the PDCD4 gene expression in response to this oxidative stress. The in vitro cultured FaDu cells were subjected to various concentrations of ONOO-, then, the cell viability and morphological changes were examined by MTT assay and acridine orange staining, respectively. The protein expressions of Caspase-9, Caspase-3, and PDCD4 were determined by western blot and the mRNA expression of PDCD4 was analyzed by RT-PCR. This work demonstrated that ONOO- could inhibit the proliferation and induce apoptosis of FaDu cells. The protein expressions of Caspase-9, Caspase-3, and PDCD4 were up-regulated and, meanwhile, the mRNA expression of PDCD4 was increased, in response to ONOO-. These data suggest that ONOO- can effectively suppress proliferation of FaDu cells via triggering the apoptotic pathway. PDCD4 gene may play an important role in ONOO--induced apoptosis in FaDu cells, which may offer a new target for the treatment of hypopharyngeal carcinoma.
关键词
NITRIC-OXIDE; INHIBITS TRANSLATION; SUPPRESSOR PDCD4; CANCER-CELLS; DEATH; EXPRESSION; PROTEIN; INITIATION; INDUCTION; BINDING
基本信息
