Combination of interferon-alpha and 5-fluorouracil induces apoptosis through mitochondrial pathway in hepatocellular carcinoma in vitro

作者

Yin, HP;Xie, FX;Zhang, J;Yang, YM;Deng, BP;Sun, JT;Wang, QJ;Qu, X;Mao, HT

作者单位

[Yin, Haipeng; Xie, Fengxiang; Yang, Yongmei; Deng, Biping; Sun, Jintang; Wang, Qingjie; Qu, Xun; Mao, Haiting] Shandong Univ, Inst Basic Med Sci, Qi Lu Hosp, Jinan 250012, Shandong, Peoples R China.;-;[Yin, Haipeng] Shandong Acad Med Sci, Inst Basic Med Sci, Jinan, Peoples R China.;-;[Zhang, Jiao] Shandong Prov Hosp, Dept Hepatopathy, Jinan, Peoples R China.

摘要

Many clinical reports have proven that the combination therapy of interferon-alpha plus 5-fluorouracil is remarkably effective for advanced hepatocellular carcinoma (HCC). However, the mechanism of this therapy is not well understood. Here, we demonstrated that the combination therapy synergistically inhibited the growth of Fas-negative HCC cells, arrested cell-cycle progression and induced apoptosis. Moreover, the combination therapy significantly increased the protein expression of caspase-8, activated Bid and cytochrome c. Meanwhile, the expression of anti-apoptotic gene Bcl-xL was reduced and intracellular calcium elevated obviously during the early stage of treatment. Therefore, mitochondria] pathway was involved in the apoptosis of Fas-negative HCC cells induced by IFN-alpha/5-FU and Ca2+ partially promoted the beneficial effect against HCC. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

关键词

DIHYDROPYRIMIDINE DEHYDROGENASE EXPRESSION; CYTOCHROME-C; CELL-GROWTH; IFN-ALPHA; INTRAARTERIAL 5-FLUOROURACIL; THERAPY; FLUOROURACIL; ACTIVATION; RECEPTOR; RELEASE

基本信息

所属机构:

归属医师: 张娇

PMID：21474236

UT：000291181100005

刊名:CANCER LETTERS

年，卷(期):2011年306卷1期

页码：34-42

DOI：10.1016/j.canlet.2011.02.017

附件：

收录: SCIE