A comparison of Twist and E-cadherin protein expression in primary non-small-cell lung carcinoma and corresponding metastases
作者
Wang, GH;Dong, W;Shen, HC;Mu, XR;Li, ZX;Lin, XY;Liu, Y;Du, JJ
作者单位
[Wang, Guanghui; Dong, Wei; Du, Jiajun] Shandong Univ, Prov Hosp, Dept Thorac Surg, Jinan 250100, Peoples R China.;-;[Shen, Hongchang; Mu, Xueru; Li, Zhenxiang] Shandong Univ, Prov Hosp, Dept Oncol, Jinan 250100, Peoples R China.;-;[Lin, Xiaoyan; Liu, Ying] Shandong Univ, Prov Hosp, Dept Pathol, Jinan 250100, Peoples R China.
摘要
Objective: The metastasis of solid tumors is directly or indirectly responsible for most cancer-related deaths. It has already been known that in non-small-cell lung carcinoma cells, up-regulation of Twist (a highly conserved basic helix-loop-helix transcription factor) can promote epithelial-mesenchymal transition through down-regulation of E-cadherin. The main aim of this study was to determine whether the expression of Twist and E-cadherin differs between primary and metastatic lung carcinoma and to correlate Twist and E-cadherin expression in primary and metastatic non-small-cell lung carcinoma. Methods: Thirteen patients with non-small-cell lung carcinoma and hematogenous metastases were studied in retrospect, and Twist and E-cadherin were detected by immunohistochemistry in 26 tissue samples from the 13 patients. Results: We demonstrated that the expression of Twist was higher in metastatic non-small-cell lung carcinoma tissues than in primary non-small-cell lung carcinoma (p = 0.008) and discordance of Twist expression was observed in 11 (85%) patients. For E-cadherin, 12 cases (92%) showed discordance between primary tumor and metastasis (p = 0.002): E-cadherin expressed higher in the primary tumor than in the metastasis in 12 cases. We also found that increased Twist expression was correlated with decreased membranous E-cadherin expression (p = 0.009). Conclusions: Our data imply that Twist induces epithelial-mesenchymal transition in non-small-cell lung carcinoma by reducing E-cadherin, then promoting metastasis. (C) 2011 European Association for Cardio-Thoracic Surgery. Published by Elsevier B. V. All rights reserved.
关键词
EPITHELIAL-MESENCHYMAL TRANSITION; GROWTH-FACTOR RECEPTOR; TUMOR PROGRESSION; CANCER; ADENOCARCINOMAS; PATHWAYS; SURVIVAL; ADHESION
基本信息
