LINC00520 targeting miR-27b-3p regulates OSMR expression level to promote acute kidney injury development through the PI3K/AKT signaling pathway
作者
Tian, XH;Ji, YQ;Liang, YF;Zhang, J;Guan, LN;Wang, CT
作者单位
[Tian, Xinghan; Zhang, Jing; Wang, Chunting] Shandong Univ, Shandong Prov Hosp, Dept Crit Care Med, 9677 East Jingshi Rd, Jinan 250012, Shandong, Peoples R China.;-;[Tian, Xinghan; Liang, Yafeng] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Crit Care Med, Yantai, Shandong, Peoples R China.;-;[Ji, Yongqiang] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Nephrol, Yantai, Shandong, Peoples R China.;-;[Guan, Lina] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Neurol, Intens Care Unit, 20 Yuhuangding East Rd, Yantai 264000, Shandong, Peoples R China.
摘要
BackgroundAcute kidney injury (AKI) shows several kinds of disorders, which acutely harm the kidney. However, the current medical methods have limited therapeutic effects. The present study aimed to find out the molecular mechanism of AKI pathogenesis, which may provide new insights for future therapy.;-;MethodsBioinformatic analysis was conducted using the R language (AT&T BellLaboratories, University of Auckland, New Zealand) to acquire the differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) in AKI. The expression levels of RNAs and related proteins in tissues and cells were detected by quantitative real-time PCR (qRT-PCR) and western blot. Dual-luciferase reporter gene assays were performed to verify the target relationship between microRNA (miRNA) and lncRNA as well as miRNA and mRNA. Flow cytometry and tunnel assay were used to detect the cell apoptotic rate in AKI.;-;ResultsLINC00520, miR-27b-3p, and OSMR form an axis to regulate AKI. Knockdown of LINC00520 reduced acute renal injury both in vitro and in vivo. LINC00520 activated the PI3K/AKT pathway to aggravate renal ischemia/reperfusion injury, while upregulation of miR-27b-3p or downregulation of OSMR could accelerate the recovery of AKI.;-;ConclusionOverexpression of LINC00520 contributes to the aggravation of AKI by targeting miR-27b-3p/OSMR.
关键词
ONCOSTATIN M RECEPTOR; EPIDEMIOLOGY; MORTALITY; PROTECTS; MICRORNA; CELLS; RISK; AKI
基本信息
