beta cells can be generated from cytokeratin 5-positive cells after cerulein-induced pancreatitis in adult mice

作者

Shi, Q;Hong, YP;Zhang, XY;Tao, J;Wang, CY;Zhao, L;Mei, FC;You, YD;Xia, H;Xiong, XC;Wang, GR;Wang, WX

作者单位

[Shi, Qiao; Tao, Jing; Xiong, Xing-cheng] Wuhan Univ, Renmin Hosp, Dept Pancreat Surg, Wuhan 430060, Hubei, Peoples R China.;-;[Hong, Yu-pu; Wang, Chen-yang; Zhao, Liang; Mei, Fang-chao; You, Yun-dong; Xia, He; Wang, Wei-xing] Wuhan Univ, Renmin Hosp, Dept Gen Surg, 238 Jiefang Rd, Wuhan 430060, Hubei, Peoples R China.;-;[Hong, Yu-pu] Key Lab Hubei Prov Digest Syst Dis, Wuhan 430060, Hubei, Peoples R China.;-;[Zhang, Xiao-yi] Shandong Univ, Shandong Prov Hosp, Dept Crit Care Med, Jinan 250021, Shandong, Peoples R China.;-;[Zhang, Xiao-yi; Wang, Gui-rong] SUNY Upstate Med Univ, Dept Surg, Syracuse, NY 13210 USA.;-;[You, Yun-dong; Xia, He] Wuhan Univ, Renmin Hosp, Cent Lab, Wuhan 430060, Hubei, Peoples R China.

摘要

Clinical studies have revealed that some patients will develop glucose tolerance dysfunction after recovering from acute pancreatitis (AP), which indicated the importance of investigating the potential therapies for restoration of islet beta cell function. Cytokeratin 5 (Krt5)-positive cells are considered to function as stem or progenitor cells in the regeneration of lung and salivary gland following injury. In the present study, AP was induced by six hourly intraperitoneal injections of 100 mu g/kg cerulein for 4 consecutive days in adult mice, in order to determine the role of Krt5-positive cells in pancreatic regeneration, especially in the restoration of beta cell function and the underlying mechanisms. Results showed that glucose homeostasis were deteriorated partly during the recovery process after AP. Furthermore, clusters of Krt5-positive cells were significantly increased in the damaged pancreas marked by inflammatory cells infiltration and acinar cell eradication. In addition, cells co-labelling insulin and Krt5 were found in the injured region after cerulein administration, part of these cells were immuno-positive for GLUT2. Taken together, our data demonstrated that Krt5-expressing cells could be involved in the natural pancreas self-healing process and the renewal of beta cells after AP in adult mice. It is promising that promoting conversion of Krt5-expressing cells into functional beta cells may be a novel method to mitigate the development of diabetes mellitus after AP in vivo. (C) 2018 Elsevier Inc. All rights reserved.

关键词

1ST-ATTACK ACUTE-PANCREATITIS; AIRWAY STEM-CELLS; LUNG REGENERATION; DIABETES-MELLITUS; UNITED-STATES; EPIDEMIOLOGY; ENDOCRINE; DISEASE; BURDEN; MODEL

基本信息

所属机构:

归属医师: 张小艺

PMID：29307825

UT：000424068000019

刊名:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

年，卷(期):2018年496卷1期

页码：114-119

DOI：10.1016/j.bbrc.2018.01.008

附件： other

收录: SCIE