Sevoflurane improves gaseous exchange and exerts protective effects in lipopolysaccharide-induced lung injury in mice models

作者

Shen, WM;Li, C;Yuan, YH;Xu, YX;Chi, YL

作者单位

[Shen, Wei-Min] Shandong Tradit Chinese Med Univ, Shandong Prov Hosp, Dept Pharm, Jinan 250014, Shandong, Peoples R China.;-;[Li, Chen] Shandong Tradit Chinese Med Univ, Shandong Prov Hosp, Dept Spine Surg, Jinan 250014, Shandong, Peoples R China.;-;[Yuan, Yan-Hua] Shandong Tradit Chinese Med Univ, Shandong Prov Hosp, Dept Well Known Specialist, Jinan 250014, Shandong, Peoples R China.;-;[Xu, Ying-Xue; Chi, Yong-Liang] Shandong Tradit Chinese Med Univ, Affiliated Hosp, Dept Anesthesiol, Jinan 250014, Shandong, Peoples R China.

摘要

Purpose: To investigate the protective effect of sevoflurane against lipopolysaccharide (LPS)-induced acute liver injury (ALI) in amice model.;-;Methods: Seven week-old female BalB/C mice were used. Lung water content and cell count were estimated by standard protocols. Cytokine and chemokine analysis was performed using commercially available kits. Myeloperoxidase activity was evaluated spectrophotometrically while histopathological analysis was carried out by H and E staining.;-;Results: The results revealed that sevoflurane treatment significantly improved gaseous exchange, and reduced lung water content and lung inflammation as evidenced by a decrease in neutrophil migration into BALF (p < 0.01). Sevoflurane also significantly reversed the LPS-triggered suppression of IL-10 in the lung tissues of LPS-treated mice, when compared to saline-treated controls (p < 0.01). It reversed LPS-induced oxidative stress, as demonstrated by increase in total antioxidant capacity (T-AC), catalase (CAT) and superoxide dismutase-1 (SOD-1), as well as an increase in reduced/oxidized glutathione (GSH/GSSG) ratio. In addition, sevoflurane blocked LPS-induced lung tissue injury in ALI mice, and exerted protective effects against acute LPS-induced lung injury.;-;Conclusion: These results suggest that sevoflurane improves gaseous exchange and exerts a protective effect against LPS-triggered lung injury in mice model, most probably due to its anti-inflammatory and antioxidant properties.

关键词

ALVEOLAR EPITHELIAL-CELLS; INFLAMMATION; ANTIOXIDANT

基本信息

所属机构:西药房

归属医师: 袁艳华申卫民李晨

UT：000426762300008

刊名:TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

年，卷(期):2018年17卷1期

页码：47-52

DOI：10.4314/tjpr.v17i1.8

附件：

收录: SCIE