Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-kappa B-mediated by RhoA/ROCK signalling pathway
作者
Wang, QL; Shen, ZW; Qi, GH; Zhao, YF; Zhang, HG; Wang, R
作者单位
[Wang, Qinglian; Zhao, Yanfang; Wang, Rong] Shandong Univ, Dept Nephrol, Shandong Prov Hosp, 324 Jingwu St, Jinan 250021, Peoples R China; [Shen, Zhenwei] WuXi Clin Dev Serv Shanghai Co Ltd, Dept Biostat, Shanghai, Peoples R China; [Qi, Guanghui] First Hosp Zibo, Dept Urol Surg, Zibo, Shandong, Peoples R China; [Zhang, Hongge] Tengzhou Hosp Tradit Chinese Med, Dept Urol Surg, Zaozhuang, Peoples R China
摘要
Advanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed that stimulation with AGE could significantly activate RhoA/NF-kappa B pathway. Results showed thymol could markedly suppress inflammatory responses, cell apoptosis and disordered cytoskeleton. Also thymol restored the expression of podocin, restrained migration capacity. Western blot analysis indicated that it could restore the expression of RhoA, ROCK and vimentin, nephrin, podocin and p65 and I kappa B alpha phosphorylation. Moreover, si-RhoA also suppressed the expression of pro-inflammatory cytokines, ROCK, and vimentin and the phosphorylation of p65 and I kappa B alpha. In conclusion, thymol inhibits AGE-induced cell injury in HPCs by suppressing the RhoA-NF-kappa B pathway and may be apromising therapeutic agent.
关键词
GLYCATION END-PRODUCTS; RENIN-ANGIOTENSIN SYSTEM; ACUTE LUNG INJURY; DIABETIC-NEPHROPATHY; PATHOPHYSIOLOGY; COMPLICATIONS; PATHOGENESIS; ADHESION
基本信息
