MicroRNA-324-5p affects the radiotherapy response of cervical cancer via targeting ELAV-like RNA binding protein 1
作者
Fan, MJ; He, PJ; Lin, XY; Yang, CR; Li, CZ; Xing, LG
作者单位
[Fan, Ming-Jun; Xing, Li-Gang] Shandong First Med Univ & Shandong Acad Med Sci, Dept Radiat Oncol, Shandong Canc Hosp & Inst, 440 Jiyan Rd, Jinan 250117, Shandong, Peoples R China; [Fan, Ming-Jun; Li, Chang-Zhong] Shandong Univ Tradit Chinese Med, Postdoctoral Mobile Stn, Jinan, Shandong, Peoples R China; [Fan, Ming-Jun; He, Peng-Juan; Lin, Xue-Yan; Yang, Chun-Run; Li, Chang-Zhong] Shandong First Med Univ, Dept Obstet & Gynecol, Shandong Prov Hosp, Jinan, Shandong, Peoples R China
摘要
Cervical cancer (CC) seriously threatens the health of women. Radiation therapy (RT) is the major treatment for CC. However, the recurrent CC can acquire resistance to RT. Thus, it is necessary to find a new method for reversing RT resistance in CC. It has been reported that microRNA-324-5p (miR-324-5p) can suppress the progression of multiple cancers. However, whether it can reverse resistance to RT in CC remains unclear. qRT-PCR and Western blotting were used to detect gene and protein expression in CC cells, respectively. Cell proliferation was tested by CCK-8 assay and colony formation assay. In addition, cell apoptosis was detected by flow cytometry. Transwell assays were performed to detect cell migration. Dual luciferase reporter assay and TargetScan were used to explore the targets of miR-324-5p. MiR-324-5p was downregulated in CC cells. Overexpression of miR-324-5p sensitized CC cells to RT. In addition, miR-324-5p mimics significantly induced apoptosis and inhibits the migration of CC cells in the presence of(137)Cs ionizing radiation. Furthermore, miR-324-5p sensitized CC cells to ionizing radiation by targeting ELAV-like RNA binding protein 1 (ELAVL1). MiR-324-5p overexpression affects the radiotherapy response of CC by targeting ELAVL1, which may serve as a new target for the treatment of CC.
关键词
METASTASIS; EXPRESSION; GROWTH
基本信息
