Electrochemical detection of ACE2 as a biomarker for diagnosis of COVID-19 and potential male infertility

作者

Liu, S; Han, LP; Li, JL; Li, H

作者单位

[Liu, Shuai] Shandong First Med Univ, Dept Urol, Shandong Prov Hosp, Jinan 250021, Shandong, Peoples R China; [Han, Liping] Shandong Police Hosp, Dept Neurol, Jinan 250002, Shandong, Peoples R China; [Li, Jinlong] Nanjing Univ Chinese Med, Hosp Nanjing 2, Dept Lab Med, Nanjing 210003, Peoples R China; [Li, Hao] Univ Jinan, Sch Biol Sci & Technol, Jinan 250024, Peoples R China

摘要

Knowing how heavily the body is burdened by SARS-CoV-2 infection is all important to avoid tragic outcomes. This demands fast and convenient assays with minimum requirement for instruments and reagents. Therefore, a short synthetic peptide is developed to perform direct serum assay, using portable hand-held potentiostat, in a reagent-less manner. The target is angiotensin converting enzyme 2 (ACE2), a protein secreted by the body into the blood to restrict viral invasion. Specifically, under electrochemical potential scanning, the peptide can covalently capture ACE2 from the serum, and then form a covalent gel-like 2D protein network with the serum proteins, in an ACE2-specific fashion. This formation of a covalent biosensing complex enables sensitive detection in serum samples of SARS-CoV-2 infected patients. The detected serum level of ACE2 can not only serve as an index of viral load, but may also hint at the associated risk of potential male infertility. These results may point to field application of this simple design in the clinical practice in treating COVID-19 in the near future.

关键词

基本信息

所属机构:泌尿外

归属医师: 刘帅

PMID：34861523

UT：000729415800008

刊名:BIOSENSORS & BIOELECTRONICS

年，卷(期):2022年198卷期

页码：-null

DOI：10.1016/j.bios.2021.113788

附件： other

收录: SCIE