Candida Exerts Fluconazole-Resistant Effect via Regulating the Expression of CDR and ERG11 in Patients with Vaginitis

作者

Liu, XH; Meng, YF; Nie, XJ; Li, XY; Tan, LY; Chen, LM

作者单位

[Liu, Xiuhong; Tan, Linyan] Peoples Hosp Yuxi City, Dept Dermatol, Yuxi 653100, Yunnan, Peoples R China; [Meng, Yunfang; Nie, Xiaojuan; Chen, Lamei] Shandong First Med Univ, Shandong Prov Hosp, Dept Dermatol, Jinan 250000, Shandong, Peoples R China; [Li, Xiaoying] Shandong First Med Univ, Shandong Prov Hosp, Dept Lab Med, Jinan 250000, Shandong, Peoples R China

摘要

Vulvovaginitis candidiasis (VVC) is a common fungal infection in women and is usually caused by Candida albicans. Fluconazole is widely used to treat VVC in clinics. However, drug resistance of C. albicans to antifungal azoles is widely developed, which has gradually caused public concerns. This study aimed to investigate the fluconazole resistance of C. albicans and its mechanism. Two hundred and sixty-three yeast isolates were collected from patients with VVC in China and determined their species distribution and susceptibility using the microdilution susceptibility test and Rosco disk diffusion method. Real-time PCR and western blot analyses were used to detect the relevant genes. C. albicans was considered the main yeast in patients with VVC, then C. glabrata, C. tropicalis, etc. Among 175 isolates of C. albicans, 13 isolates were found to have fluconazole resistance (7.43%). Besides, CDR1 was over-expressed in all fluconazole-resistant isolates, while the up-regulation of CDR2 was found in 9 isolates. Moreover, ERG11 was up-regulated in 5 isolates, while MDR1 was increased in 6 isolates, and FLU1 was highly expressed in 6 isolates. In addition, the protein expression of Cdr1 and Cdr2 was generally checked in a fluconazole-resistant isolate, while Cdr2 was not expressed in C3. The fluconazole resistance of vaginal isolates was a complicated problem involved in various mechanisms, which need further studies.

关键词

基本信息

所属机构:

归属医师: 孟云芳聂小娟陈腊梅

UT：000681049400033

刊名:LATIN AMERICAN JOURNAL OF PHARMACY

年，卷(期):2021年40卷8期

页码：1923-

附件：

收录: SCIE