Pandemic influenza A (H1N1) virus causes abortive infection of primary human T cells

作者

Yu, JP; Li, H; Jia, J; Huang, ZS; Liu, S; Zheng, Y; Mu, SR; Deng, XY; Zou, XH; Wang, YM; Shang, X; Cui, D; Huang, LX; Feng, XX; Liu, WJ; Cao, B

作者单位

[Yu, Jiapei; Deng, Xiaoyan; Shang, Xiao] Tsinghua Univ, Sch Med, Dept Basic Med Sci, Beijing, Peoples R China; [Yu, Jiapei; Deng, Xiaoyan; Shang, Xiao; Cao, Bin] Tsinghua Univ, Tsinghua Univ Peking Univ Joint Ctr Life Sci, Beijing, Peoples R China; [Li, Hui; Jia, Ju; Huang, Zhisheng; Zheng, Ying; Mu, Shengrui; Zou, Xiaohui; Wang, Yeming; Cui, Dan; Huang, Lixue; Cao, Bin] China Japan Friendship Hosp, Ctr Resp Med, Dept Pulm & Crit Care Med, Beijing, Peoples R China; [Li, Hui; Zou, Xiaohui; Cao, Bin] China Japan Friendship Hosp, Natl Clin Res Ctr Resp Med, Lab Clin Microbiol & Infect Dis, Beijing, Peoples R China; [Li, Hui; Jia, Ju; Huang, Zhisheng; Cao, Bin] Chinese Acad Med Sci & Peking Union Med Coll, Inst Resp Med, Beijing, Peoples R China; [Li, Hui; Zheng, Ying; Mu, Shengrui; Wang, Yeming; Huang, Lixue; Cao, Bin] Capital Med Univ, Clin Ctr Pulm Infect, Dept Pulm & Crit Care Med, Beijing, Peoples R China; [Liu, Shuai] Shandong First Med Univ, Dept Resp & Crit Care Med, Shandong Prov Hosp, Jinan, Peoples R China; [Cui, Dan] Harbin Med Univ, Dept Resp Med, Harbin, Peoples R China; [Feng, Xiaoxuan] Zhengzhou Univ, Dept Resp Intens Care Unit, Affiliated Hosp 1, Zhengzhou, Peoples R China; [Liu, William J.] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, NHC Key Lab Biosafety, Beijing, Peoples R China

摘要

Influenza A virus still represents a noticeable epidemic risk to international public health at present, despite the extensive use of vaccines and anti-viral drugs. In the fight against pathogens, the immune defence lines consisting of diverse lymphocytes are indispensable for humans. However, the role of virus infection of lymphocytes and subsequent abnormal immune cell death remains to be explored. Different T cell subpopulations have distinct characterizations and functions, and we reveal the high heterogeneity of susceptibility to viral infection and biological responses such as apoptosis in various CD4(+) T and CD8(+) T cell subsets through single-cell transcriptome analyses. Effector memory CD8(+) T cells (CD8(+) T-EM) that mediate protective memory are identified as the most susceptible subset to pandemic influenza A virus infection among primary human T cells. Non-productive infection is established in CD8(+) T-EM and naive CD8(+) T cells, which indicate the mechanism of intracellular antiviral activities for inhibition of virus replication such as abnormal viral splicing efficiency, incomplete life cycles and up-regulation of interferon-stimulated genes in human T cells. These findings provide insights into understanding lymphopenia and the infectious mechanisms of pandemic influenza A virus and broad immune host-pathogen interactional atlas in primary human T cells.

关键词

基本信息

所属机构:

归属医师: 刘帅

PMID：35317717

UT：000787074400001

刊名:EMERGING MICROBES & INFECTIONS

年，卷(期):2022年11卷1期

页码：1191-

DOI：10.1080/22221751.2022.2056523

附件： other

收录: SCIE