Prime Editing in Mammals: The Next Generation of Precision Genome Editing
作者
Wang, DW; Fan, XD; Li, MZ; Liu, TB; Lu, P; Wang, GX; Li, Y; Han, JM; Zhao, JJ
作者单位
[Wang, Dawei; Li, Mengzhu; Zhao, JiaJun] Shandong Univ, Shandong Prov Hosp, Dept Endocrinol, Jinan, Peoples R China; [Wang, Dawei; Fan, Xiude; Li, Mengzhu; Liu, Tianbo; Lu, Peng; Li, Yuan; Han, JunMing; Zhao, JiaJun] Shandong First Med Univ, Shandong Prov Hosp, Dept Endocrinol, Jinan, Peoples R China; [Wang, Dawei; Fan, Xiude; Li, Mengzhu; Liu, Tianbo; Lu, Peng; Li, Yuan; Han, JunMing; Zhao, JiaJun] Shandong Engn Res Ctr Stem Cell & Gene Therapy End, Jinan, Peoples R China; [Wang, Dawei; Fan, Xiude; Li, Mengzhu; Liu, Tianbo; Lu, Peng; Li, Yuan; Han, JunMing; Zhao, JiaJun] Shandong Key Lab Endocrinol & Lipid Metab, Jinan, Peoples R China; [Wang, Guangxin] Shandong First Med Univ, Cent Hosp, Shandong Innovat Ctr Intelligent Diag, Jinan, Peoples R China
摘要
The recently established prime editor (PE) system is regarded as next-generation gene-editing technology. This methodology can install any base-to-base change as well as insertions and deletions without the requirement for double-stranded break formation or donor DNA templates; thus, it offers more targeting flexibility and greater editing precision than conventional CRISPR-Cas systems or base editors. In this study, we introduce the basic principles of PE and then review its most recent progress in terms of editing versatility, specificity, and efficiency in mammals. Next, we summarize key considerations regarding the selection of PE variants, prime editing guide RNA (pegRNA) design rules, and the efficiency and accuracy evaluation of PE. Finally, we highlight and discuss how PE can assist in a wide range of biological studies and how it can be applied to make precise genomic corrections in animal models, which paves the way for curing human diseases.
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