Polyunsaturated fatty acids promote the rapid fusion of lipid droplets in Caenorhabditis elegans
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摘要
Lipid droplets (LDs) are intracellular organelles that dynamically regulate lipids and energy homeostasis in the cell. LDs can grow through either local lipid synthesis or LD fusion. However, how lipids involving in LD fusion for LD growth is largely unknown. Here, we show that genetic mutation of acox-3 (acyl-CoA oxidase), maoc-1 (enoyl-CoA hydratase), dhs-28 (3-hydroxylacyl-CoA dehydrogenase), and daf-22 (3-ketoacyl-CoA thiolase), all involved in the peroxisomal beta-oxidation pathway in Caenorhabditis elegans, led to rapid fusion of adjacent LDs to form giant LDs (gLDs). Mechanistically, we show that dysfunction of peroxisomal beta-oxidation results in the accumulation of long-chain fatty acid-CoA and phosphocho-line, which may activate the sterol-binding protein 1/sterol regulatory element-binding protein to promote gLD forma-tion. Furthermore, we found that inactivation of either FAT-2 (delta-12 desaturase) or FAT-3 and FAT-1 (delta-15 desaturase and delta-6 desaturase, respectively) to block the biosynthesis of polyunsaturated fatty acids (PUFAs) with three or more double bonds (n >= 3-PUFAs) fully repressed the formation of gLDs; in contrast, dietary supplementation of n >= 3-PUFAs or phosphocholine bearing these PUFAs led to recovery of the formation of gLDs in peroxisomal beta-oxidation-defective worms lacking PUFA biosynthesis. Thus, we conclude that n >= 3-PUFAs, distinct from other well-known lipids and proteins, promote rapid LD fusion leading to LD growth.
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