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Caveolin-1 is overexpressed in hypopharyngeal squamous cell carcinoma and correlates with clinical parameters
Zhao, XN;Yu, G;Yu, XM;Wang, J;Pan, XL
ONCOLOGY LETTERS 2016年 12卷4期 页码:2371-2374
PROSTATE-CANCER; LIPID RAFTS; EXPRESSION; GROWTH; BREAST; INHIBITION; DOMAINS; GENE
The present study aimed to identify the role of caveolin-1 (CAV1) in hypopharyngeal squamous cell carcinoma (HSCC) and identify its possible correlation with tumor clinical parameters. Expression of CAV1 was measured using immunohistochemical staining of 66 HSCC samples and 44 samples from morphologically normal tissues adjacent to the carcinomas. Expression of CAV1 in HSCC and paracancerous tissues were 71.2 and 9.5% respectively. Levels of CAV1 expression were significantly associated with tumor differentiation, tumor-node-metastasis stage and lymph nodes metastasis (P<0.05). The present study identified that expression of CAV1 in HSCC is significantly higher than in paracancerous tissues, suggesting that this high expression of CAV1 is associated with tumor invasion and metastasis.
Immunotherapy for Lewis lung carcinoma utilizing dendritic cells infected with CK19 gene recombinant adenoviral vectors
Sun, QF;Zhao, XN;Peng, CL;Hao, YT;Zhao, YP;Jiang, N;Xue, H;Guo, JZ;Yun, CH;Cong, B;Zhao, XG
ONCOLOGY REPORTS 2015年 34卷5期 页码:2289-2295
POLYMERASE-CHAIN-REACTION; MESSENGER-RNA EXPRESSION; CYTOTOXIC T-LYMPHOCYTES; GROWTH-FACTOR RECEPTOR; IN-VITRO; ANTITUMOR IMMUNITY; TUMOR VACCINES; BREAST-CANCER; ANTIGENS; INDUCTION
Dendritic cells (DCs) as 'professional' antigen-presenting cells (APCs) initiate and regulate immune responses to various antigens. DC-based vaccines have become a promising modality in cancer immunotherapy. Cytokeratin 19 (CK19) protein is expressed at high levels in lung cancer and many other tumor cells, suggesting CK19 as a potential tumor-specific target for cancer immune therapy. We constructed a recombinant adenoviral vector containing the CK19 gene (rAd-CK19). DCs transfected with rAd-CK19 were used to vaccinate C57BL/6 mice bearing xenografts derived from Lewis lung carcinoma (LLC) cells. The transfected DCs gave rise to potent CK19-specific cytotoxic T lymphocytes (CTLs) capable of lysing LLC cells. Mice immunized with the rAd-CK19-DCs exhibited significantly attenuated tumor growth (including tumor volume and weight) when compared to the tumor growth of mice immunized with rAd-c DCs or DCs during the 24-day observation period (P<0.05). The results revealed that the mice vaccinated with the rAd-CK19-DCs exhibited a potent protective and therapeutic antitumor immunity to LLC cells in the subcutaneous model along with an inhibitive effect on tumor growth compared to the mice vaccinated with the rAd-c DCs or DCs alone. The present study proposes a meaningful mode of action utilizing rAd-CK19 DCs in lung cancer immunotherapy.
Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro
Yu, XM;Li, CW;Chao, SS;Li, YY;Yan, Y;Zhao, XN;Yu, FG;Liu, J;Shen, L;Pan, XL;Shi, L;Wang, DY
SCIENTIFIC REPORTS 2014年 4卷
CHRONIC RHINOSINUSITIS; UPPER AIRWAYS; UP-REGULATION; STEM-CELLS; EXPRESSION; DISEASE; REPAIR; TIME
Basal cells in nasal epithelium have stemness/progenitor characters and play essential roles in the epithelial remodeling in nasal polyps (NP). We investigate whether the human nasal epithelial stem/progenitor cells (hNESPCs) from patients with NP are inherently distinct from those obtained from healthy controls. Epithelial basal cells were isolated and cultured for four passages from NP tissues and control nasal mucosa. hNESPCs from controls were stained positively with stem cell marker p63 and KRT5 and presented a consistent high Ki67 expression level over four passages. In contrast, hNESPCs from NP patients showed: i). a reduced growth and proliferation rate at each passage by evaluating colony-forming efficiency and doubling time; ii). a lower percentage of Ki67(+) cells among p63(+) cells in the colonies in late passages, which was also confirmed by immunostaining in the NP tissues. Thus reduced growth/proliferation dynamics in hNESPCs from NP could be an important pathological phenomenon in NP development.
Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem-like cell properties by high aldehyde dehydrogenase activity
Yu, FG;Sim, ACN;Li, CW;Li, YY;Zhao, XN;Wang, DY;Loh, KS
LARYNGOSCOPE 2013年 123卷8期 页码:1903-1911 影响因子:2.272
EPSTEIN-BARR-VIRUS; ACUTE MYELOID-LEUKEMIA; BREAST CANCERS; 1 EXPRESSION; MARKER; CHEMOTHERAPY; METASTASIS; INHIBITION; RESISTANCE; PREDICTOR
Objectives/Hypothesis Cancer stem cells have been reported as a new therapeutic target in many cancers, but their existence in nasopharyngeal carcinoma (NPC) is largely unknown. This study was conducted to determine cancer stem-like cells in NPC cell line.;-;Study Design Basic science experimental study.;-;Methods Aldehyde dehydrogenase (ALDH) activity, a putative functional marker for cancer stem cells, was assessed in Epstein-Barr virus-associated NPC cell line C666-1 cells. The ability of cells with high and low ALDH activity to proliferate, resist therapy, and initiate tumor formation was compared.;-;Results Enrichment of cancer stem-like cells (with high ALDH activity) in C666-1 was associated with a significantly greater ability to proliferate, be clonogenic, resist chemotherapy drugs and radiation, reconstitute a heterogeneous population, and express pluripotent markers. Furthermore, subcutaneous injection of these cells into immunodeficient nude mice resulted in a tendency of tumor formation at a higher rate as compared to cells with low ALDH activity.;-;Conclusions These results provide evidence for the existence of cancer stem-like cells in the NPC cell line C666-1 cells.
Inferior vestibular neuritis: a novel subtype of vestibular neuritis
Zhang, D;Fan, Z;Han, Y;Yu, G;Wang, H
JOURNAL OF LARYNGOLOGY AND OTOLOGY 2010年 124卷5期 页码:477-481
RECOVERY; NERVE
Objective: To report eight cases of inferior vestibular neuritis, in order to raise awareness of this new subtype of vestibular neuritis.;-;Materials and methods: We retrospectively analysed 216 patients 04 males and 112 females; age range 10-64 years; mean age 38.4 years) with full clinical documentation who had attended our hospital's vertigo clinic between May 2007 and December 2008. All patients underwent systematic investigation, including hearing tests, radiology, caloric testing and vestibular evoked myogenic potential testing.;-;Results: Of 216 patients with vestibular neuritis, eight cases were diagnosed as inferior vestibular neuritis, based on comprehensive analysis of test data. The clinical features of these eight patients were consistent with the characteristics of vestibular neuritis. The results of pure tone audiometry and caloric testing were normal, and the possibility of central lesions was excluded by cerebral computed tomography or magnetic resonance imaging on admission. Six cases had unilateral loss of vestibular evoked myogenic potentials, whereas two had a unilateral lower amplitude of vestibular evoked myogenic potentials.;-;Conclusions: Inferior vestibular neuritis is a novel subtype of vestibular neuritis, which involves the inferior vestibular nerve alone. Vestibular evoked myogenic potential testing is a useful aid to the diagnosis of inferior vestibular neuritis.
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