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熊去氧胆酸选择性诱导人肝肿瘤细胞凋亡及抑制增殖的实验研究
刘慧 [1] 韩国庆 [1] 孟玫 [1] 秦成勇 [1] 许洪伟 [1] 赖宁 [2]
《山东医药》 2004年 44卷34期 页码:10-12页
肝肿瘤 细胞株 熊去氧胆酸 细胞凋亡
目的探讨熊去氧胆酸(UDCA)诱导肝肿瘤细胞株凋亡并抑制其增殖的作用和机制.方法用噻唑蓝法、流式细胞术、TUNEL法、Wright-Giemsa染色法、电镜及免疫细胞化学等方法,观察UDCA对肝肿瘤细胞株HepG2、BEL7402和正常人肝细胞株L-02的生长活力、细胞凋亡、细胞周期及Bax/bcl-2基因表达的影响.结果 UDCA对HepG2、BEL7402细胞株具有显著的抑制生长、诱导凋亡、阻滞细胞周期于S期、降低bcl-2和提升Bax表达的作用.结论 UDCA对HepG2、BEL7402细胞株有显著的抑制增殖及诱导凋亡作用,该作用可能与UDCA阻滞细胞周期、降低bcl-2和提升Bax的表达有关.
两种链格孢霉变应原提取方法的比较
曹乃清;张宏誉;杨秀云;高鲁芳
中华临床免疫和变态反应杂志 2009年 3卷1期 页码:21-24页,共4页 影响因子:0.885
传统方法;新方法;链格孢霉变应原浸出液;生物活性;放射性变应原吸附抑制试验
目的 比较传统方法和新方法对链格孢霉变应原浸出液蛋白质含量、组分及生物活性的影响.方法 将经空气曝皿获得、中国科学院鉴定为链格孢霉的菌株于26℃恒温下培养4局,取12份5g湿重的菌苔,6份用传统方法(风干,高速粉碎机粉碎后,搅拌提取),6份采用新方法(液氮研磨+超声破碎,再搅拌提取)提取蛋白质;然后用改良的Bradford法进行蛋白质含量测定;SDS-PAGE分析两种方法提取的蛋白质组分的差别,放射性变应原吸附(RAST)抑制试验比较两者间变应原生物活性的差异.结果 传统方法和新方法提取的蛋白质含量分别为(0.205±0.019)和(0.532±0.023)g/ml,所得到变应原的50%抑制率分别为5.96和1.25μg/ml.结论 两种提取方法中,新方法能够得到较高的蛋白质含量及更多的蛋白质组分,对于低丰度蛋白的获得及生物学活性即效价的提高具有十分重要的意义.
两种链格孢霉变应原提取方法的比较
曹乃清;张宏誊;杨秀云;高鲁芳
中华临床免疫和变态反应杂志 2009年1期 影响因子:0.885
传统方法;新方法;链格孢霉变应原浸出液;生物活性;放射性变应原吸附抑制试验
目的比较传统方法和新方法对链格孢霉变应原浸出液蛋白质含量、组分及生物活性的影响。方法将经空气曝皿获得、中国科学院鉴定为链格孢霉的菌株于26℃恒温下培养4周,取12份5 g湿重的菌苔,6份用传统方法(风干,高速粉碎机粉碎后,搅拌提取),6份采用新方法(液氮研磨+超声破碎,再搅拌提取)提取蛋白质;然后用改良的Bradford法进行蛋白质含量测定:SDS-PAGE分析两种方法提取的蛋白质组分的差别,放射性变应原吸附(RAST)抑制试验比较两者间变应原生物活性的差异。结果传统方法和新方法提取的蛋白质含量分别为(0.205±0.019)和(0.532±0.023)g/ml,所得到变应原的50%抑制率分别为5.96和1.25μg/ml。结论两种提取方法中,新方法能够得到较高的蛋白质含量及更多的蛋白质组分,对于低丰度蛋白的获得及生物学活性即效价的提高具有十分重要的意义。
Deficiency of HTR4 and ADRB1 caused by SARS-CoV-2 spike may partially explain multiple COVID-19 related syndromes including depression, cognitive impairment, loss of appetite, heart failure, and hypertension
Lv, L; Li, A; Jiang, L; Zhang, LL
JOURNAL OF INFECTION 2023年 86卷2期 页码:191-
Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition
An, S; Liu, WJ; Fu, JW; Zhang, Z; Zhang, RL
PARASITES & VECTORS 2023年 16卷1期 页码:-null
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Background Aedes albopictus is an increasingly serious threat in public health due to it is vector of multiple arboviruses that cause devastating human diseases, as well as its widening distribution in recent years. Insecticide resistance is a serious problem worldwide that limits the efficacy of chemical control strategies against Ae. albopictus. Chitinase genes have been widely recognized as attractive targets for the development of effective and environmentally safe insect management measures. Methods Chitinase genes of Ae. albopictus were identified and characterized on the basis of bioinformatics search of the referenced genome. Gene characterizations and phylogenetic relationships of chitinase genes were investigated, and spatio-temporal expression pattern of each chitinase gene was evaluated using qRT-PCR. RNA interference (RNAi) was used to suppress the expression of AaCht10, and the roles of AaCht10 were verified based on phynotype observations, chitin content analysis and hematoxylin and eosin (H&E) stain of epidermis and midgut. Results Altogether, 14 chitinase-related genes (12 chitinase genes and 2 IDGFs) encoding 17 proteins were identified. Phylogenetic analysis showed that all these AaChts were classified into seven groups, and most of them were gathered into group IX. Only AaCht5-1, AaCht10 and AaCht18 contained both catalytic and chitin-binding domains. Different AaChts displayed development- and tissue-specific expression profiling. Suppression of the expression of AaCht10 resulted in abnormal molting, increased mortality, decreased chitin content and thinning epicuticle, procuticle and midgut wall of pupa. Conclusions Findings of the present study will aid in determining the biological functions of AaChts and also contribute to using AaChts as potential target for mosquito management.
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