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The correlation between occupational stress and redox state among nurse population
Mao, JB;Zhou, XY;Duan, HX;Li, C;Wang, Z
BIOMEDICAL RESEARCH-INDIA 2017年 28卷13期 页码:6013-6017
DISEASE; CANCER; SYSTEM
Background: This research is aimed to explore the influence of occupational stress on oxidation and oxidation resistance in nurse population.;-;Methods: 140 nurses were included in this research. The questionnaire was performed to collect the data of occupational stress. Moreover, the hydroxyl radical, antioxidant enzyme level was tested.;-;Results: Superoxide Dismutase (SOD) level in nurses younger than 30 y old was significantly higher than other groups while that was most lower in nurses older than 45 y old. The difference was significant (P<0.05). Compared with participants with lower academic degrees, nurses with higher degree had a significantly higher Glutathione Peroxidase (GSH-Px) level. The work prospect and work control was the main influence factors for SOD. Work risk was the negative factor while peace emotion was the positive factor of SOD (P<0.05).;-;Conclusion: The occupational stress, such as the work prospect and work control is associated with body's anti-oxidation ability.
DHA increases the anti-tumor effect of gefitinib on non-small cell lung cancer with EGFR mutations in vitro
Ren, WG;Wu, J;Wang, XH;Feng, Z;Shang, XC;Peng, ZM
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 2017年 10卷5期 页码:7647-7657
POLYUNSATURATED FATTY-ACIDS; DOCOSAHEXAENOIC ACID; GROWTH; CHEMOTHERAPY; EXPRESSION; CONSUMPTION; APOPTOSIS; PATHWAYS; THERAPY; DISEASE
Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) are approved as first-line therapy for patients with non-small cell lung cancer (NSCLC) harboring EGFR activating mutations. Docosahexaenoic acid (DHA) exerts anti-neoplastic activity in human lung cancer cells. In this study, we investigated whether DHA increases the anti-tumor effects of gefitinib on NSCLC cells with EGFR mutations and the related mechanisms of action. We determined the effects of DHA and gefitinib on the proliferation, apoptosis, cell cycle, and signaling pathways of NSCLC cells with EGFR activating mutations (PC9 cells) and TKI resistance (A549 cells). DHA had an obvious inhibitory effect on both cell lines, and enhanced the anti-tumor effects of gefitinib on the cells in vitro. Combined gefitinib and DHA therapy had a synergistic effect, inducing apoptosis, causing G0/G1 arrest in the PC9 cells and affecting EGFR and ERK1/2 signaling. These results suggest that DHA can act as a sensitizer of gefitinib in NSCLC cells with EGFR mutations. Nutritional intervention with DHA is a promising approach to enhancing the therapeutic effect of gefitinib.
4-cholesten-3-one suppresses lung adenocarcinoma metastasis by regulating translocation of HMGB1, HIF1 alpha and Caveolin-1
Ma, JB;Fu, GB;Wu, J;Han, SX;Zhang, LS;Yang, M;Yu, Y;Zhang, MY;Lin, YL;Wang, YB
CELL DEATH & DISEASE 2016年 7卷
METHYL-BETA-CYCLODEXTRIN; LIPID RAFTS; CHOLESTEROL DEPLETION; CANCER METASTASIS; DOWN-REGULATION; TUMOR-GROWTH; APOPTOSIS; CELL; OSTEOSARCOMA; ACTIVATION
Metastasis is a great challenge in lung adenocarcinoma (ADC) therapy. Cholesterol has been implicated in ADC metastasis. 4-cholesten-3-one, as cholesterolmetabolite and analog, can substitutemembrane cholesterol and increase membrane fluidity. In this study, we explored the possibility that 4-cholesten-3-one inhibited ADC metastasis. Low-dose 4-cholesten-3-one significantly restrained ADC cells migration and invasion with little effects on cells viabilities. Further investigation showed that 4-cholesten-3-one promoted ROS generation, which transiently activated AMPK alpha 1, increased HIF1 alpha expression, reduced Bcl-2 expression and caused autophagy. AMPKa1 knockdown partly suppressed 4-cholesten-3-one-induced autophagy but, neither prevented 4-cholesten-3-one-induced upregulation of HIF1 alpha or downregulation of Bcl-2. 4-cholesten-3-one-induced autophagy facilitated the release of HMGB1 from nuclei to cytoplasm, blocking nuclear translocation of HIF1 alpha and activation of MMP2 and MMP9. Also, 4-cholesten-3-one induced time-dependent phosphorylation of caveolin-1, Akt and NF-kappa B. With increasing treatment time, 4-cholesten-3-one accelerated caveolin-1 internalization, but reduced the phosphorylation of Akt and NF-kappa B, and inhibited the expression of snail and twist. These data suggested that 4-cholesten-3-one could be a potential candidate for anti-metastasis of lung adenocarcinoma.
Aberrant expression of Notch1/numb/snail signaling, an epithelial mesenchymal transition related pathway, in adenomyosis
Qi, SS;Zhao, XB;Li, MJ;Zhang, XH;Lu, ZZ;Yang, CR;Zhang, CH;Zhang, H;Zhang, N
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 2015年 13卷
E-CADHERIN EXPRESSION; ENDOMETRIAL STROMAL CELLS; CANCER-CELLS; TRANSCRIPTION FACTORS; PROSTATE-CANCER; CARCINOMA-CELLS; OVARIAN-CANCER; SNAIL; NUMB; NOTCH1
Background: Epithelial mesenchymal transition (EMT) is involved in the pathogenesis of adenomyosis, and Notch signaling is crucial to EMT. The objective of this study was to explore Notch1/Numb/Snail signaling in adenomyosis.;-;Methods: The expression levels of the members of the Notch1/Numb/Snail signaling cascade in normal endometria (proliferative phase: n = 15; secretory phase: n = 15; postmenopausal phase: n = 15) and adenomyotic endometria (proliferative phase: n = 15; secretory phase: n = 15) were determined by immunohistochemistry analysis.;-;Results: We found that the expressions of Notch1 and the EMT-related proteins N-cadherin, Snail and Slug were upregulated in the ectopic endometrium of adenomyosis compared with normal endometrium. Numb, a negative regulator of Notch signaling, was significantly decreased in adenomyosis. In addition, reduced immunoexpression of E-cadherin was observed in adenomyosis.;-;Conclusions: We conclude that Notch1/Numb/Snail signaling plays an important role in the pathogenesis and development of adenomyosis.
Mechanism Analysis for the Influence of Propofol's Therapeutic Window by Purpurin
Xing, CY;Wu, MY;Li, W;Zhou, XY;Zhang, MY
LATIN AMERICAN JOURNAL OF PHARMACY 2014年 33卷7期 页码:1210-1212
HYPNOTIC AGENT PROPOFOL; STRONG INHIBITION; GLUCURONIDATION; METABOLISM
Drug-drug interaction (DDI) strongly limits the clinical application of propofol which has been clinically used as a short-acting, intravenously administered hypnotic agent. The present study aims to determine the inhibition of propofol glucuronidation by purpurin which is a natural anthraquinone pigment isolated from madder root (Rubia tinctorum L.). The results showed that purpurin exhibited concentration-dependent inhibition towards the glucuronidation of propofol, and noncompetitive inhibition behavior of purpurin towards propofol glucuronidation was demonstrated by inhibition kinetic study (Dixon plot). Given that UGT1A9 was the major drug-metabolizing enzyme involved in the metabolism of propofol, detailed mechanism was furtherly investigated through evaluating the inhibition of purpurin towards recombinant UGT1A9-catalyzed 4-methylumbelliferone. The concentration-dependent inhibition of purpurin towards the formation of 4-MU glucuronide was also detected, demonstrating the inhibition of purpurin towards the activity of UGT1A9. All these results showed that purpurin affected the therapeutic window of propofol thorough influence of the activity of UGT1A9, reminding the clinical importance to monitor the drug-drug interaction when co-administering propofol and purpurin.
乳腺癌患者部分外周血指标的变化及肿瘤标志物的诊断价值
张璐璐;刘芸;段文冰;唐闻;李晓莹;赵万辉;张炳昌
中国医药 2018年 13卷3期 页码:421-425,共5页 影响因子:0.941
乳腺癌;;红细胞体积分布宽度;;癌胚抗原;;糖类抗原125;;糖类抗原153;;
目的 探讨乳腺癌患者血常规、血脂、血生化等指标的变化及血清糖类抗原125、糖类抗原153、癌胚抗原联合检测对乳腺癌的诊断价值.方法 选取2016年8月至2017年5月山东大学附属省立医院收治的女性乳腺癌患者92例(乳腺癌组,其中有淋巴结转移59例、无淋巴结转移33例)、女性乳腺良性肿瘤患者41例(乳腺良性肿瘤组)、体检健康女性50名(健康对照组),采用化学发光法检测3组研究对象癌胚抗原、糖类抗原125、糖类抗原153的含量,并对乳腺癌组及健康对照组外周血红细胞体积分布宽度(RDW)、血细胞比容(HCT)、中性粒细胞计数(NEU)与淋巴细胞计数(LYM)的比值(NLR)、血清唾液酸、前白蛋白、糖化血清蛋白(GSP)和血脂进行检测.结果 ①乳腺癌组的RDW、NLR、唾液酸、GSP、总胆固醇、三酰甘油均高于健康对照组,HCT、LYM、前白蛋白、高密度脂蛋白胆固醇均低于健康对照组(均P<0.05).无淋巴结转移组RDW、NEU、NLR、GSP均低于有淋巴结转移组,LYM、前白蛋白高于有淋巴结转移组(均P<0.05).②乳腺癌组的糖类抗原125、糖类抗原153、癌胚抗原含量明显高于乳腺良性肿瘤组和健康对照组(均P<0.05).乳腺癌组3项联合检测的阳性率高于癌胚抗原、糖类抗原125、糖类抗原153单独检测[43.5% (40/92)比15.2%(14/92)、14.1% (13/92)、23.9% (22/92)](均P<0.05).③在3项肿瘤标记物中,糖类抗原153诊断乳腺癌的敏感度为23.91%,特异度为98.00%,准确度为50.00%,阴性预测值为41.18%,阳性预测值为95.61%,是单项检测中最佳的乳腺癌诊断指标;3项联合检测的敏感度为43.48%,准确度为61.97%,阴性预测值为48.00%.3项肿瘤标志物联合诊断乳腺癌的受试者工作特征曲线下面积大于癌胚抗原、糖类抗原125单独检测(0.805比0.704、0.701)(P<0.05),与糖类抗原153的曲线下面积(0.757)比较差异无统计学意义(P>0.05).结论 乳腺癌患者RDW、NLR、GSP等指标对乳腺癌的转移有着重要的参考价值.癌胚抗原、糖类抗原125、糖类抗原153联合检测大大提高了乳腺癌早期诊断阳性率.
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