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Human epidermal growth factor receptor 2 amplification detection by droplet digital polymerase chain reaction in formalin-fixed paraffin-embedded breast and gastric cancer samples
Wang, XW;Wu, YY;Song, XL;Sun, CT;Wu, CS;Feng, H
JOURNAL OF CANCER RESEARCH AND THERAPEUTICS 2017年 13卷4期 页码:730-734
PRACTICE GUIDELINE UPDATE; OF-AMERICAN-PATHOLOGISTS; CLINICAL-ONCOLOGY/COLLEGE; HER2; PCR; SOCIETY; RECOMMENDATIONS; EXPRESSION; MICRODISSECTION; QUANTITATION
Objective: Human epidermal growth factor receptor 2 (HER2) is an important biomarker for the precise individualized treatment including trastuzumab of HER2-positive breast and gastric cancer. Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) are the routine analyses for formalin-fixed paraffin-embedded (FFPE) samples. However, IHC is variable and depends on the evaluator, and FISH is a labor intensive and expensive method. We evaluated the feasibility of droplet digital polymerase chain reaction (ddPCR) as a precise and quantitative method for HER2 amplification test. Materials and Methods: We used ddPCR to confirm HER2 amplification status in 24 breast cancer and 29 gastric cancer samples to validate the HER2 cutoff value in ddPCR. After setting cutoff value, all the above-mentioned samples were tested by IHC. Afterward, another 51 equivocal IHC 2+ gastric cancer samples were further determined by FISH and ddPCR, respectively, and the concordance between ddPCR and FISH was calculated. Results: We set the HER2 cutoff value at 1.8. The concordance rate of HER2 status between ddPCR and IHC was 94.4% (17 out of 18) in 24 breast cancer samples. In 29 gastric cancer specimens, the concordance rate of HER2 amplification between ddPCR and IHC was 100% (22 out of 22). At last, compared with FISH determined HER2 status, ddPCR HER2 scores correctly classified 44 of 51 cases with 86.3% concordance in 51 equivocal IHC 2+ gastric cancer samples. Conclusions: ddPCR was able to identify HER2 amplification status in breast and gastric cancers with precise correlation with IHC and FISH results. This method might become a standard method for testing FFPE samples. However, the technology requires further research.
糖尿病性白内障患者人工晶状体植入术后保护动机干预模式的研究
吴云燕;李莉;王好莎
中国实用护理杂志 2017年 33卷10期 页码:725-729,共5页 影响因子:1.951
白内障;;糖尿病;;视力,眼睛;;有晶状体眼人工晶状体植入术;;保护动机;;
目的 探讨保护动机干预在糖尿病性白内障患者人工晶状体植入术后康复中的作用.方法 选取糖尿病性白内障患者118例,按随机数字表法分为观察组与对照组,每组59例.2组患者住院时均行常规护理模式,观察组同时给予保护动机干预.评估2组患者围手术期血糖变化、术后视力恢复及生命质量指数情况.结果 观察组患者术日晨、术后1 d空腹血糖分别为(8.27±1.25)、(9.52±2.44)mmol/L,对照组分别为(10.49±1.48)、(12.77±3.36)mmol/L,2组比较差异有统计学意义(t=-5.392、-6.755,P<0.01).观察组术后1、3个月视力分别为0.64±0.22、0.67±0.30,明显高于对照组的0.56±0.18、0.58±0.20,且观察组出现术后盲或低视力率为3.95%(3眼),明显低于对照组的13.51%(10眼),2组比较差异有统计学意义(t/χ2=2.492、2.209、4.013,均P<0.05).观察组生命质量指数为(7.70±1.13)分,对照组生命质量指数为(5.22±1.48)分,2组比较差异有统计学意义(t=3.660,P<0.05).结论 保护动机干预能明显提高糖尿病性白内障患者术后视力,减少并发症的发生,提高患者生命质量.
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