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The auditory characteristics of children with inner auditory canal stenosis
Ai, Y;Xu, L;Li, L;Li, JF;Luo, JF;Wang, MM;Fan, ZM;Wang, HB
ACTA OTO-LARYNGOLOGICA 2016年 136卷7期 页码:687-691
NEUROPATHY SPECTRUM DISORDER; COCHLEAR NERVE; TEMPORAL BONE; HYPOPLASIA; APLASIA; ABNORMALITIES
Conclusions This study shows that the prevalence of auditory neuropathy spectrum disorder (ANSD) in the children with inner auditory canal (IAC) stenosis is much higher than those without IAC stenosis, regardless of whether they have other inner ear anomalies. In addition, the auditory characteristics of ANSD with IAC stenosis are significantly different from those of ANSD without any middle and inner ear malformations. Objectives To describe the auditory characteristics in children with IAC stenosis as well as to examine whether the narrow inner auditory canal is associated with ANSD. Method A total of 21 children, with inner auditory canal stenosis, participated in this study. A series of auditory tests were measured. Meanwhile, a comparative study was conducted on the auditory characteristics of ANSD, based on whether the children were associated with isolated IAC stenosis. Results Wave V in the ABR was not observed in all the patients, while cochlear microphonic (CM) response was detected in 81.1% ears with stenotic IAC. Sixteen of 19 (84.2%) ears with isolated IAC stenosis had CM response present on auditory brainstem responses (ABR) waveforms. There was no significant difference in ANSD characteristics between the children with and without isolated IAC stenosis.
Reproducibility of quantitative real-time PCR assay in microRNA expression profiling and comparison with microarray analysis in narcolepsy
Liu, ZH;Yang, LL;Zhao, YZ;Tang, ML;Wang, FM;Wang, XT;Li, GZ;Du, YF
SPRINGERPLUS 2015年 4卷 影响因子:0.982
SLEEP; WAKEFULNESS; DISORDERS; MECHANISM; THERAPY; BRAIN; RISK
MicroRNAs (miRNAs) have been shown in the pathogenesis of human neurological disorders. The study aims to identify the involvement of miRNAs in the pathophysiology of narcolepsy. Here, we conducted three independent high-throughput analysis of miRNA (miRNA microarray) in peripheral blood from 20 narcolepsy patients who fulfilled the criteria compared to 20 healthy controls with validation experiment using quantitative real-time polymerase chain reaction (real-time PCR) panels. By analyzing 2805 miRNAs in peripheral blood with microarray we identified 128 miRNAs (105 high expression and 23 low expression) that were different in patients with narcolepsy in comparison with healthy control. Then we chose six high expression candidates and six low expression candidates of at least twofold difference and p value < 0.05 to validate the changes in three independent experiments in vitro using real-time PCR. The validation test showed that levels of hsa-mir-1267, hsa-miR-4309, hsa-miR-554, hsa-miR-1272, hsa-miR-4501, hsamiR- 182-3p were higher, whereas the level of hsa-miR-625-5p, hsa-miR-100-5p, hsa-miR-125b-5p, hsa-miR-197-3p, hsa-miR-4522, hsa-miR-493-5p was lower in narcolepsy patients than healthy controls. The levels of 12 miRNAs differed significantly in peripheral blood from narcolepsy patients which suggested that alterations of miRNAs expression may be involved in the pathophysiology of narcolepsy.
Expression of Surfactant Protein-A during LPS-Induced Otitis Media with Effusion in Mice
Li, L;Guo, XR;Olszewski, E;Fan, ZM;Ai, Y;Han, Y;Xu, L;Li, JF;Wang, HB
OTOLARYNGOLOGY-HEAD AND NECK SURGERY 2015年 153卷3期 页码:433-439
PULMONARY SURFACTANT; EUSTACHIAN-TUBE; TNF-ALPHA; INFLAMMATORY RESPONSE; UP-REGULATION; LUNG; CYTOKINES; PHAGOCYTOSIS; MACROPHAGES; EPITHELIUM
Objective The objective of this study was to investigate the expression and role of surfactant protein (SP) in the middle ear throughout lipopolysaccharide (LPS)-induced otitis media with effusion (OME).;-;Study Design Randomized case-controlled animal model.;-;Setting Shandong University, Jinan, China.;-;Subjects and Methods SP expression was monitored using reverse transcription polymerase chain reaction (PCR) in normal mice (n = 5). Two groups, control phosphate-buffered saline-injected mice (n = 5) and LPS-injected mice (n = 5), were euthanized 5 days following injection. RNA was extracted for reverse transcription PCR and real-time PCR, and temporal bone samples were used for hematoxylin and eosin staining. LPS was injected into mice, and 5 mice per test were euthanized at 0, 12, 24, 48, 72, and 96 hours following injection. For mRNA expression quantification, reverse transcription PCR and real-time PCR were performed, and proinflammatory cytokine levels were measured by enzyme-linked immunosorbent assay.;-;Results SP-A and SP-D expression was detected in normal murine Eustachian tubes. SP-A expression was up-regulated after LPS-induced OME (P = .01). At various time points after LPS injection, concentrations of proinflammatory cytokines (tumor necrosis factor-[TNF-], interleukin (IL)-1, and IL-6) in the middle ear increased significantly (P < .05) and correlated with changes in SP-A expression.;-;Conclusion SP-A and SP-D exist in the murine middle ear. The expression of SP-A and TNF-, IL-1, and IL-6 was up-regulated in the middle ear of the LPS-induced OME animal model.
Surgical management of facial paralysis resulting from temporal bone fractures
Liu, YQ;Han, J;Zhou, XC;Gao, K;Luan, DH;Xie, FY;Wang, XT;Zong, GX;Ding, L
ACTA OTO-LARYNGOLOGICA 2014年 134卷6期 页码:656-660
DECOMPRESSION SURGERY; NERVE PARALYSIS; OUTCOMES; PALSY
Conclusion: To achieve good facial reanimation in cases with facial paralysis resulting from temporal bone fractures, the ideal timing for surgical intervention is at least within 1 month of injury and an appropriate surgical approach should be selected depending on the site of facial nerve injury. Objective: This paper aimed to address the ideal time for surgical intervention and the appropriate surgical approach for patients with facial paralysis resulting from temporal bone fractures. Methods: We retrospectively investigated 60 patients with facial paralysis due to temporal bone fractures who underwent facial nerve decompression via different operative approaches within 1 month after trauma, of which 48 were surgically treated by the middle cranial fossa approach (80%), 8 by a pure transmastoid approach (13.3%), and four by a combined transmastoid and middle cranial fossa approach (6.7%). The House-Brackmann (H-B) grading system was used to evaluate the recovery of facial nerve function. Results: The follow-up period for all the patients was 1 year. Among 60 patients who were surgically treated, 39 achieved grade I of facial nerve function, 18 achieved grade II, two achieved grade III, and one achieved grade IV according to the H-B grading system.
Prestin at year 14: Progress and prospect
He, DZZ;Lovas, S;Ai, Y;Li, Y;Beisel, KW
HEARING RESEARCH 2014年 311卷 页码:25-35
OUTER HAIR-CELLS; MOTOR PROTEIN PRESTIN; GUINEA-PIG COCHLEA; MULTIFUNCTIONAL ANION-EXCHANGERS; CYSTEINE MUTAGENESIS REVEALS; RESONANCE ENERGY-TRANSFER; ATOMIC-FORCE MICROSCOPY; PLASMA-MEMBRANE; VOLTAGE-SENSOR; SEQUENCE EVOLUTION
Prestin, the motor protein of cochlear outer hair cells, was identified 14 years ago. Prestin-based outer hair cell motility is responsible for the exquisite sensitivity and frequency selectivity seen in the mammalian cochlea. Prestin is the 5th member of an eleven-member membrane transporter superfamily of SLC26A proteins. Unlike its paralogs, which are capable of transporting anions across the cell membrane, prestin primarily functions as a motor protein with unique capability of performing direct and reciprocal electromechanical conversion on microsecond time scale. Significant progress in the understanding of its structure and the molecular mechanism has been made in recent years using electrophysiological, biochemical, comparative genomics, structural bioinformatics, molecular dynamics simulation, site-directed mutagenesis and domain-swapping techniques. This article reviews recent advances of the structural and functional properties of prestin with focus on the areas that are critical but still controversial in understanding the molecular mechanism of how prestin works: The structural domains for voltage sensing and interaction with anions and for conformational change. Future research directions and potential application of prestin are also discussed. This article is part of a Special Issue entitled . (C) 2013 Elsevier B.V. All rights reserved.
Immunomodulatory Effects of Escherichia coli ATCC 25922 on Allergic Airway Inflammation in a Mouse Model
Pang, WH;Wang, HF;Shi, L;Sun, YQ;Wang, XT;Wang, MM;Li, JF;Wang, HB;Shi, GG
PLOS ONE 2013年 8卷3期 影响因子:3.057
HYGIENE HYPOTHESIS; STEM-CELLS; EARLY-LIFE; ASTHMA; RHINITIS; MICROBIOTA; DISEASES; MICE; IMMUNOLOGY; MECHANISMS
Background: Hygiene hypothesis demonstrates that the lack of microbial exposure would promote the development of allergic airway disease (AAD). Therefore, the gut microbiota, including Escherichia coli (E. coli), would probably offer a potential strategy for AAD.;-;Objective: To investigate whether E. coli infection is able to suppress the induction of AAD and to elucidate the underlying mechanisms.;-;Methods: Nonpathogenic E. coli ATCC 25922 was infected by gavage before AAD phase in three patterns: 10(8) or 10(6) CFU in neonates or 10(8) CFU in adults. Then mice were sensitized and challenged with ovalbumin (OVA) to induce allergic inflammation in both the upper and lower airways. Hallmarks of AAD, in terms of eosinophil infiltration and goblet cell metaplasia in subepithelial mucosa, Th2 skewing of the immune response, and levels of T regulate cells (Tregs), were examined by histological analysis, ELISA, and flow cytometry, respectively.;-;Results: E. coli, especially neonatally infected with an optimal dose, attenuated allergic responses, including a decrease in nasal rubbing and sneezing, a reduction in eosinophil inflammation and goblet cell metaplasia in subepithelial mucosa, decreased serum levels of OVA-specific IgE, and reduced Th2 (IL-4) cytokines. In contrast, this effect came with an increase of Th1 (IFN-r and IL-2) cytokines, and an enhancement of IL-10-secreting Tregs in paratracheal lymph nodes (PTLN).;-;Conclusion: E. coli suppresses allergic responses in mice, probably via a shift from Th1 to Th2 and/or induction of Tregs. Moreover, this infection is age-and dose-dependent, which may open up novel possibilities for new therapeutic interventions.
Factors affecting recurrence of sinonasal inverted papilloma
Xiao-Ting, W;Peng, L;Xiu-Qing, W;Hai-Bo, W;Wen-Hui, P;Bing, L;Er-Peng, Z;Guang-Gang, S
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY 2013年 270卷4期 页码:1349-1353
无关键词信息
The aim is to study clinical characteristics and recurrence rates for sinonasal inverted papilloma (NIP), to evaluate relevant factors for its recurrence, and to compare the curative rates of different surgical approaches. The Krouse classification for the 156 follow-up cases of the patients with NIP was as following: 26 cases in T1, 33 cases in T2, 94 cases in T3, and 3 cases in T4. A total number of 99 cases of endoscopic sinus surgery were included, of which 26 cases of lateral rhinotomy approach were combined with Caldwell Luc approach, and 31 cases of nasal endoscopy combined with traditional surgery, with average postoperative follow-up of 3-11 years. Of the 156 studied patients with NIP, male:female = 1.69:1, age varied from 18 to 77 years, with average of 56 years, 19 cases showed postoperative recurrence, of which 8 cases developed to be squamous cell carcinoma. Among these 8 cases, 3 patients showed no recurrence after treatment in 5 years, and the other 5 patients died in 3-2 years period. Tumor recurrence rates for different surgical approach are: 9.09 % for endoscopic surgical group, 23.08 % for traditional surgical group, and 12.12 % for combined surgical group; tumor malignancy rates for different surgical approach are: 2.02 % for endoscopic surgical group, 11.54 % traditional surgical group, and 9.09 % for combined group, and Chi-square test showed that the differences in recurrence and malignancy rates for NIP patients with different surgical treatments were not statistically significant (P > 0.05). Recurrence rates for different stages are: T1 at 3.85 %, T2 at 12.12 %, T3 at 14.89 %, and T4 at 0.00 %, and the differences in the recurrence rates for different stages were not statistically significant (P > 0.05) by Chi-square test. There are clear clinical features for NIP, and the recurrence is related to the thoroughness of the first time surgical removal of lesions and is less relevant with Krouse classification stage and surgical approaches.
Benign paroxysmal vertigo of childhood: Diagnostic value of vestibular test and high stimulus rate auditory brainstem response test
Zhang, DG;Fan, ZM;Han, YC;Wang, MM;Xu, L;Luo, JF;Ai, Y;Wang, HB
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY 2012年 76卷1期 页码:107-110
MIGRAINE EQUIVALENT; ISCHEMIA; CHILDREN
Objective: To investigate the diagnostic value of vestibular test and high stimulus rate auditory brainstem response (ABR) test and the possible mechanism responsible for benign paroxysmal vertigo of childhood (BPVC).;-;Methods: Data of 56 patients with BPVC in vertigo clinic of our hospital from May 2007 to September 2008 were retrospectively analyzed in this study. Patients with BPVC were tested with pure tone audiometry, high stimulus rate auditory brainstem response test (ABR), transcranial Doppler sonography (TCD), bithermal caloric test, and VEMP. The results of the hearing and vestibular function test were compared and analyzed.;-;Results: There were 56 patients with BPVC, including 32 men, 24 women, aged 3-12 years old, with an average of 6.5 years. Among 56 cases of BPVC patients, the results of pure tone audiometry were all normal. High stimulus rate ABR was abnormal in 66.1% (37/56) of cases. TCD showed 57.1% abnormality in 56 cases, including faster flow rate in 28 cases and slower flow rate in 4 cases. High stimulus rate ABR and TCD were both abnormal in 48.2% (27/56) of cases. Bithermal caloric test was abnormal in 14.3% (8/56) of cases. VEMP showed 32.1% abnormality, including amplitude abnormality in 16 cases and latency abnormality in 2 cases. The abnormal rate of VEMP was much higher than that of caloric test.;-;Conclusion: Vascular mechanisms might be involved in the pathogenesis of BPVC and there is strong evidence for close relationship between BPVC and migraine. High stimulus rate ABR is helpful in the diagnosis of BPVC. The inferior vestibular pathway is much more impaired than the superior vestibular pathway in BPVC. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
重度极重度聋儿童不同刺激声ASSR与行为测听听阈的一致性及相关性分析
艾毓;李文靖;徐磊;樊兆民;王海波
中华耳科学杂志 2017年 15卷2期 页码:163-167 影响因子:0.75
诱发电位;;行为测听;;感音神经性耳聋;;
目的 通过对比传统ASSR和NB-CE-Chirp刺激声ASSR诱发阈同行为测听听阈的相关性,评价重度-极重度聋儿童中,不同刺激声ASSR在客观听阈评估中的价值.方法 对重度-极重度聋儿童进行传统ASSR和NB-CE-Chirp-ASSR检查(每组各30人60耳),两组儿童均进行行为测听检查,将ASSR的诱发阈和行为测听的听阈进行比较,分析NB-CE-Chirp刺激声ASSR和传统ASSR同行为测听听阈一致性,对比两种不同刺激声ASSR同行为测听听阈的相关性.结果 NB-CE-Chirp刺激声ASSR组和传统ASSR组的诱发阈同行为测听听阈的一致性均较好,前者91.67%,后者83.33%.传统ASSR组500Hz诱发阈、NB-CE-Chirp ASSR 1000Hz、2000Hz及4000Hz诱发阈均与行为测听听阈存在中度相关,相关系数分别为0.62、0.53、0.56和0.51.其余频率呈低度相关.结论 对于重度-极重度聋儿童,同传统ASSR相比,NB-CE-Chirp ASSR能更好的反映纯音听阈.
鼻腔鼻窦肿瘤临床特征和病理组织学特点的分析
王小婷;时光刚;刘亦青;纪宏志;何明强;李建峰;王海波
临床耳鼻咽喉头颈外科杂志 2011年 25卷23期 页码:1071-1075页,共5页 影响因子:0.805
鼻肿瘤;临床,病理学;治疗
目的:研究鼻腔鼻窦肿瘤的临床特征及病理组织学特点.方法:收集活检或手术切除,经病理证实的333例鼻腔鼻窦肿瘤患者,统计其发病年龄、性别、部位分布及病理构成比,观察其临床特征及病理组织学特点.结果:①所有患者中,男200例,女133例;男女比为1.5 ∶ 1;年龄2~84岁,中位年龄54岁;良性肿瘤177例,恶性肿瘤156例,良性恶性比为1.1∶1.原发于鼻腔的肿瘤有142例;原发于鼻窦的肿瘤191例,包括上颌窦肿瘤90例,额窦肿瘤31例,筛窦肿瘤46例,蝶窦肿瘤24例,其中55例累及鼻腔.②本组鼻腔鼻窦肿瘤总的构成比按组织来源排序为上皮组织、软组织、淋巴造血组织、骨及软骨组织、异位颅内组织肿瘤;恶性肿瘤排在前5位的是鳞状细胞癌、淋巴瘤、腺样囊性癌、恶性黑素瘤、嗅神经母细胞瘤;良性肿瘤排在前5位的是乳头状瘤、纤维瘤、骨瘤、血管瘤、异位颅内良性肿瘤.③上皮组织来源者共200例,良性恶性比为1.4 ∶ 1(118∶82).软组织肿瘤共68例,良性恶性比为1.2∶1(37∶31).骨与软骨组织肿瘤22例,良性恶性比为3.4∶1(17∶5),良性者均为骨瘤.淋巴造血组织来源的肿瘤29例,以恶性为主有28例.异位颅内肿瘤也可见到,共2例.此外,还收集到其他各种类型肿瘤12例.结论:鼻腔鼻窦肿瘤有明确的临床特征,不同部位的肿瘤有不同的临床发生特点;可以发生多种组织类型的肿瘤,其中以内翻性乳头状瘤、鳞状细胞癌、纤维瘤、骨瘤、淋巴瘤、腺样囊性癌最为常见,在临床病理学上具有多样性和复杂性的特点,鼻腔鼻窦肿瘤的诊断及鉴别诊断主要依靠病理检查;治疗采取以手术为主的综合治疗.
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