在结果中搜索
- 已选条件:
Overexpression of OCT4 is associated with gefitinib resistance in non-small cell lung cancer
Li, B;Yao, ZH;Wan, YY;Lin, DJ
Oncotarget 2016年 7卷47期 页码:77342-77347 影响因子:5.008
EXPRESSION; EGFR; MUTATION; DOMAIN
Epidermal growth factor receptor (EGFR)-targeted tyrosine kinase inhibitors (TKIs) have emerged as first-line drugs for non-small cell lung cancers (NSCLCs). However, the resistance to TKIs represents the key limitation for their therapeutic efficacy. We found that the difference of OCT4 expression between NSCLC and the adjacent non-tumourous tissues was statistically significant. Knockdown of OCT4 in NSCLC cells could decrease cell proliferation, and potentiate apoptosis induced by gefitinib, suggesting OCT4 may contribute to gefitinib resistance in NSCLC.
Increased serum VDBP as a risk predictor for steroid resistance in asthma patients
Jiang, HJ;Chi, XY;Zhang, X;Wang, J
RESPIRATORY MEDICINE 2016年 114卷 页码:111-116
D-BINDING-PROTEIN; VITAMIN-D; GC-GLOBULIN; BIOMARKER; PROTEOMICS; INFLAMMATION; LUNG; 1,25-DIHYDROXYVITAMIN-D; CANDIDATE; DISEASE
Background: Asthmatic symptoms usually can be controlled with corticosteroids, but partly asthmatic patients do not respond to corticosteroids, steroid resistance (SR) play a significant role in the poorly responding. However, no approach can accurately predict steroid responsiveness in asthma patients, so prediction of SR with noninvasive means has become a critical issue.;-;Objective: The aim of this study was to evaluate the difference in serum proteomes between steroidsensitive asthma (SSA) and steroid-resistant asthma (SRA) patients and identify potential biomarkers for the prediction of SR in asthma patients.;-;Methods: We performed a proteomic approach of fluorescence-based difference gel electrophoresis (DIGE) and mass spectrometry to identify biomarkers in the serum obtained from SRA and SSA patients (n = 6 in each group). The interesting biomarker was further studied using western blot and enzyme-linked immunosorbent assays (ELISA).;-;Results: Seven differentially expressed proteins between SSA and SRA group were identified. Among them, vitamin D-binding protein (VDBP) attracted our further attention as the greatest changed protein. Serum VDBP was significantly up-regulated in SRA group compared with SSA group, and the differential expression was confirmed with western blot analysis. The ELISA data showed the serum level of VDBP was significantly higher in SRA group than that in SSA and control group (496.50 +/- 204.62 vs. 279.73 +/- 163.65, 241.93 +/- 98.58 mg/ml, respectively, p < 0.01). Correlation analysis indicated serum VDBP was positively correlated with neutrophils% and monocytes% (p < 0.05), but inversely correlate with serum 25OHD (p < 0.05). Regression analysis showed increased serum VDBP was a risk predictor of SRA, and serum 25OHD was an independent influential factor of serum VDBP. Using the receiver operating characteristic curve, we determined the area under the curve (AUC) of VDBP was 0.792, and the optimal serum cutoff value of VDBP was 355.8 mg/ml, which can discriminate SRA from asthma patients with 65.2% sensitivity and 83.7% specificity.;-;Conclusions: This study provides a novel overview of the difference in serum proteomes of SSA and SRA. We suppose serum VDBP may serve as a useful biomarker for predicting SR in asthma patients, and may participate in the pathogenesis of SRA. (C) 2016 Elsevier Ltd. All rights reserved.
Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line
Zhang, X;Jiang, SJ;Shang, B;Jiang, HJ
THORACIC CANCER 2015年 6卷2期 页码:202-208
CANCER-CELLS; ANTITUMOR-ACTIVITY; LUNG-CANCER; DOCETAXEL; PROTEIN; DEATH; INDUCTION; DISEASE; FUTURE; GENES
BackgroundHistone deacetylase (HDAC) inhibitors combined with other drugs for the treatment of malignant tumors are used more and more widely. In this study, we investigated the effect of trichostatin A (TSA), a HDAC inhibitor, in combination with cisplatin, a cytotoxic chemotherapy agent, on the apoptosis of lung cancer A549 cells.;-;MethodsA549 cells were treated with TSA alone, cisplatin alone or the two drugs combined. Cell viability and apoptosis were evaluated using a light microscope, methyl thiazolyl tetrazolium (MTT) (3-[4, 5-dimethylthiazol-2-yl] -2, 5-diphenyltetrazolium bromide) assay and Hochst33258 staining. Moreover, Western blot analysis was employed to examine the alterations of apoptosis protein: cellular Fas-associated death domain-like interleukin-1 converting enzyme inhibitory protein (cFLIP) and caspase-8 in A549 cells in response to the different exogenous stimuli.;-;ResultsCompared with single-agent treatment, the co-treatment of A549 cells with TSA and cisplatin synergistically inhibited cell proliferation, induced apoptosis, and increased the inhibition rate. Treatment with TSA and cisplatin led to a significant decrease of cFLIP expression. Furthermore, the treatment of A549 cells with TSA and cisplatin resulted in a significant decrease of pro-caspase-8 and a significant increase of caspase-8.;-;ConclusionsSynergistic anti-tumor effects are observed between cisplatin and TSA in lung cancer cells. The combination of TSA with cisplatin may be a more effective method in human lung cancer treatment.
Correlation between CYP4F2 gene rs2108622 polymorphism and susceptibility to ischemic stroke
Meng, C;Wang, J;Ge, WN;Tang, SC;Xu, GM
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 2015年 8卷9期 页码:16122-16126
SINGLE NUCLEOTIDE POLYMORPHISM; BLOOD-PRESSURE; WARFARIN; METAANALYSIS; ASSOCIATION; POPULATION; EXCRETION; CYP4A11; VKORC1; CYP2C9
Objective: To conduct a meta-analysis for the correlation between cytochrome P450 4F2 (CYP4F2) rs2108622 (V433M) gene polymorphism and ischemic stroke. Methods: We retrieved the case-control studies on the correlation between CYP4F2 V433M polymorphism and ischemic stroke included in domestic and international databases before January 2015 and selected the best genetic model, using RevMan 5.2 software for meta-analysis. According to the heterogeneity test results of selected literature, the effect model of consolidated data was selected, and the combined OR and 95% CI were calculated. Results: A total of six documents were included. Recessive model (VM + MM vs. VV) was selected as the best genetic model. The combined results showed that: compared with wildtype VV, there are significant association between ischemic stroke and CYP4F2 polymorphism (OR merge = 1.37, 95% CI: 1.21 similar to 1.54, P < 0.001). Conclusion: CYP4F2 V433M may be the susceptibility gene for ischemic stroke.
Overexpression of Numb suppresses tumor cell growth and enhances sensitivity to cisplatin in epithelioid malignant pleural mesothelioma
Kang, YM;Ding, MJ;Tian, GY;Guo, HS;Wan, YY;Yao, ZH;Li, B;Lin, DJ
ONCOLOGY REPORTS 2013年 30卷1期 页码:313-319
APOPTOSIS PROTEINS; LUNG-CANCER; STEM-CELLS; IN-VIVO; EXPRESSION; RESISTANCE; INHIBITOR; MECHANISMS; FAMILY; CARCINOGENESIS
Malignant pleural mesothelioma (MPM) is a highly aggressive and conventional treatment-resistant tumor with a dismal prognosis. Among the three histological subtypes of MPM, the epithelioid is the most common type. Numb is considered as a tumor suppressor playing a critical role in controlling asymmetric cell division, maintenance of stem cell compartments, ubiquitination of specific substrates and regulating Notch-, Hedgehog- and TP53-activated pathways. The present study was designed to analyze the role of Numb in epithelioid MPM. We investigated the expression of Numb in 39 epithelioid MPM and 22 normal pleural tissues by immunohistochemistry. Furthermore, we overexpressed Numb in NCI-H2452, an epithelioid human MPM cell line, and investigated the effect of Numb overexpression on the proliferation, apoptosis and sensitivity to cisplatin in cells. The expression of Numb was significantly lower in MPM compared to the control group and Numb had an inverse correlation with the Ki-67 labeling index. Loss of Numb expression was associated with poor prognosis in epithelioid MPM. Overexpression of Numb in NCI-H2452 cells significantly inhibited proliferation, promoted apoptosis and enhanced sensitivity to cisplatin. Moreover, Numb overexpression activated caspase-9 and caspase-3 through release of cytochrome c as well as downregulation of XIAP and survivin. We speculate that cytochrome c/caspase signaling is a possible mechanism through which Numb enhances the apoptosis of NCI-H2452 cells. These results suggest that Numb may be involved in epithelioid MPM development, and its upregulation may confer sensitivity to cisplatin, suggesting potential therapeutic options for MPM.
Association of ADAM33 gene polymorphisms with asthma in a Chinese population
Chi, XY;Wang, LC;Wang, J;Li, Q;Wang, XQ;Wang, JP;Xiao, W
CLINICAL RESPIRATORY JOURNAL 2013年 7卷1期 页码:16-20
NECROSIS-FACTOR-ALPHA; JAPANESE POPULATION; BRONCHIAL HYPERRESPONSIVENESS; DISINTEGRIN; EXPRESSION; CELLS
Background and Aim: Asthma is a very common disease involving genetic and environmental factors. A disintegrin and metalloproteinase 33 (ADAM33) has been one of the most exciting candidate genes for asthma since its first association with the disease in the white population. Recently, studies on the association of ADAM33 gene polymorphisms with the risk of asthma have been controversial. We therefore focused on testing the hypothesis that either single-nucleotide polymorphisms (SNPs) of the ADAM33 gene may be associated with asthma risk. The aim of this study was to evaluate the potential relationship between polymorphisms of ADAM33 and asthma in a Han population in China. Methods: A case control study was conducted in a Han population of eastern Chinese population. A total of 329 asthma patients and a control group of 316 healthy volunteers were recruited for this study. Four polymorphic sites (F+1, S2, T2 and V4) were selected for genotyping. Genotypes were determined by the allele-specific polymerase chain reaction with fluorescence melting curves and DNA sequencing method. Data were analysed using the chi-squared test software. Results: Statistically significant differences in the distributions of the S2 site between patients and controls were observed (2=7.140, P<0.05). Conclusion: These preliminary results suggest an association between ADAM33 polymorphisms S2 C/G and asthma in a Chinese Han population. The SNPs (F+1 C/T, T2 G/A and V4 C/G) of the ADAM33 gene may be the causal variants in asthma disease, but the strength of this evidence is limited by our small sample size. Please cite this paper as: Chi X, Wang L, Wang J, Li Q, Wang X, Wang J and Xiao W. Association of ADAM33 gene polymorphisms with asthma in a Chinese population. Clin Respir J 2013; 7: 1620.
Effect of Glucocorticoid in mice of asthma induced by ovalbumin sensitisation and RSV infection
Chi, XY;Jiang, SJ;Wang, J;Wang, JP
ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY 2011年 29卷2期 页码:176-180
MIGRATION INHIBITORY FACTOR; MURINE MODEL; AIRWAY INFLAMMATION; RESPONSES; CELLS
Objective: To investigate the inflammatory changes and the airway hyper-responsiveness in the asthma mouse model infected by respiratory syncytial virus and elucidate the relationship between the infection and the effect of glucocorticoid.;-;Methods: 60 BALB/c mice were randomly divided into 6 groups. One of these is the control group; the others are the OVA/sham group, the OVA/sham +Dex group, the PBS/RSV group, the OVA/RSV group and the OVA/RSV+Dex group. The airway resistance was measured using a sealed body plethysmograph. Pathological slides were stained with hematoxylin-eosin, and the peribronchial inflammation was observed microscopically. The concentrations of IL-4, IFN-gamma, TGF-beta(1) in lung tissues were detected by ELISA.;-;Results: Compared with the control group, the degree of the airway inflammation and hyperresponsiveness and the concentrations of IL-4/IFN-gamma,. TGF-beta(1) in all four OVA groups increased significantly. And there was a statistically significant difference between the OVA/sham group and the OVA/sham+Dex group, and between the OVA/RSV group and the OVA/RSV+Dex group respectively. Compared with the OVA/RSV group, there was an obvious aggravation of airway inflammation and hyperresponsiveness in the OVA/RSV+Dex group.;-;Conclusions: Glucocorticoid significantly reduces airway inflammation and hyper-responsiveness induced by repetitive OVA challenge in the mouse model of asthma. However, the significant decrease in Th-1 and increase in Th-2 inflammation and aggravation of airway hyper-responsiveness in the mice in OVA/RSV group show that they are not sensitive to glucocorticoid. The effects of infection with RSV on the mouse model of asthma could be the cause of the glucocorticoid resistance during the therapy. (Asian Pac J Allergy Immunol 2011;29:176-80)
Thoracoscopy for diagnosis and management of refractory hepatic hydrothorax
Lin, DJ;Zhang, M;Gao, GX;Li, B;Wang, MF;Zhu, L;Xue, LF
CHINESE MEDICAL JOURNAL 2006年 119卷5期 页码:430-434
沙美特罗替卡松粉吸入剂联合太极拳锻炼对稳定期重度COPD患者的影响
李青;房卫华;刘翠;迟翔宇
国际呼吸杂志 2017年 37卷1期 页码:41-44,共4页 影响因子:0.419
稳定期;;慢性阻塞性肺疾病;;沙美特罗替卡松粉吸入剂;;太极拳;;
目的 探讨沙美特罗替卡松粉吸入剂(舒利迭)联合太极拳锻炼对稳定期重度COPD患者的影响.方法 将稳定期重度COPD患者76例随机分组为观察组和对照组各38例,脱落6例,最终入组各35例.2组在常规治疗基础上,观察组给予沙美特罗替卡松粉吸入剂50 μg/500 μg吸入及太极拳锻炼.对照组只给予沙美特罗替卡松粉吸入剂50 μg/500 μg吸入.2组疗程均为6个月,并随访至少1年统计门诊及住院次数.结果 2组在肺功能、活动能力、生活质量方面均较前明显改善(P<0.05),观察组免疫功能明显高于对照组(P<0.05),门诊及住院次数明显低于对照组(P<0.05).结论 沙美特罗替卡松粉吸入剂联合太极拳锻炼不仅能改善稳定期重度COPD患者的肺功能、活动能力和生活质量,还能提高患者免疫功能,减少门诊及住院次数.
舒尼替尼与吉西他滨联合及序贯应用对 K-RAS 突变 A549 细胞的影响
孙杰;牟晓燕;董雪丽
山东大学学报:医学版 2014年 52卷3期 页码:45-49页,共6页
舒尼替尼;吉西他滨;K-RAS突变;肺腺癌
目的 研究舒尼替尼与吉西他滨单药、联合及序贯应用对人肺腺癌A549细胞增殖及凋亡的作用及其机制。方法 实验分为对照组、舒尼替尼组、吉西他滨组、舒尼替尼序贯吉西他滨组、吉西他滨序贯舒尼替尼组和联合组。A549细胞经舒尼替尼与吉西他滨单药、联合及序贯作用后,MTT法检测细胞增殖 Hoechst33258染色法检测细胞凋亡形态 流式细胞术检测细胞凋亡及周期分布 Westernblotting检测磷酸化细胞外调节蛋白激酶(PERK1/2)及磷酸化蛋白激酶B(PAKT)的表达。结果 A549细胞对舒尼替尼耐药,对吉西他滨敏感。与吉西他滨组相比,吉西他滨序贯舒尼替尼组A549细胞增殖抑制率及凋亡率明显增高(P〈0.05)。舒尼替尼与吉西他滨分别阻滞A549细胞于G1期及S期。与对照组相比,舒尼替尼序贯吉西他滨组G1期细胞增多(P〈0.05),S期细胞减少(P〈0.05)。与舒尼替尼组相比,吉西他滨序贯舒尼替尼组A549细胞PERK1/2与PAKT的表达降低(P〈0.05)。结论 吉西他滨序贯舒尼替尼作用于A549细胞具有协同作用,其机制与酪氨酸激酶受体信号通路的表达有关。
每页: 条
- <<
- <
- >
- >>