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Diffusion kurtosis imaging for differentiating parotid tumors
Yu, JF;Zhang, Q;Lu, Y;Wang, XS;Wu, WH;Zhang, WD;Zhang, DS;Wang, GB;Li, CT
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 2017年 10卷5期 页码:8025-8030
PLEOMORPHIC ADENOMAS; WEIGHTED MRI; GLAND; CANCER; FEASIBILITY; PARAMETERS; EXPERIENCE; ANATOMY; SURGERY; MODEL
Objective: Fine needle aspiration biopsy is invasive and may lead to spread of tumor cells and a higher possibility of local recurrence, especially for pleomorphic adenomas and malignant tumors. The purpose of this study was to elucidate the utility of diffusion kurtosis imaging (DKI) for differentiation of parotid tumors. Methods: 34 parotid tumors were examined with conventional magnetic resonance imaging and DKI. Data of DKI were analyzed with a diffusion kurtosis estimator to calculate mean kurtosis (MK), mean diffusivity (MD), and fractional anisotropy (FA). The diagnostic accuracy of MK, MD and FA values was evaluated, including sensitivity, specificity, and area under receiver operating characteristic (ROC) curve (AUC). Results: There were significant differences of benign and malignant parotid tumors in the values of MK, FA and MD (P=0.003, 0.019 and 0.047). Mean MK value of benign parotid tumors was significantly lower than that of malignant parotid tumors (0.728 +/- 0.263 vs. 1.091 +/- 0.253, respectively). Mean FA value of benign parotid tumors was significantly lower than malignant parotid tumors, and mean MD value of benign tumors was significantly higher than malignant tumors. The cut-off point between benign and malignant parotid tumors for MK was 1.053. The sensitivity, specificity and AUC for MK were 75.000%, 91.300% and 0.853, respectively. The AUC for FA and MD in differentiating benign and malignant parotid tumor was 0.783 and 0.739. The sensitivity and specificity for FA and MD were 75.000%, 82.610% and 50.000%, 95.650%, respectively. Conclusion: DKI showed higher specificity and sensitivity than conventional diffusion-weighted and tensor imaging for assessment of benign and malignant parotid tumors. MK enables differentiation and characterization of parotid tumors.
Application of DTI and ARFI imaging in differential diagnosis of parotid tumours
Yu, JF;Du, YF;Lu, Y;Zhang, WD;Zhang, DS;Wang, GB;Li, CT
DENTOMAXILLOFACIAL RADIOLOGY 2016年 45卷6期
RADIATION FORCE IMPULSE; DIFFUSION-WEIGHTED MRI; SALIVARY-GLAND TUMORS; PLEOMORPHIC ADENOMAS; LIVER FIBROSIS; HUMAN BRAIN; LESIONS; SONOELASTOGRAPHY; ULTRASOUND; PREDICTION
Objectives: To explore the utility of diffusion tensor imaging (DTI) and acoustic radiation force impulse (ARFI) imaging in the diagnosis of parotid tumours.;-;Methods: 51 patients with parotid tumours were examined with DTI on 3.0-T MRI and ARFI imaging on an ultrasound scanner before surgery. Values of apparent diffusion coefficient (ADC), fractional anisotropy (FA) and shear-wave velocity (SWV) were calculated and analyzed with independent samples Wilcoxon-Mann-Whitney test. Cut-off values, sensitivity and specificity were calculated with receiver-operating characteristic (ROC) curve analysis. The value of combination was calculated through parallel test for the cut-off value of ADC, FA and SWV (combination = 0 or 1); then, ROC analysis was performed with pathological results as the gold standard to calculate the sensitivity and specificity for the combination of the three parameters distinguishing benign and malignant parotid tumours. Pathological diagnosis for every patient was made post-operatively from the tumour tissue taken during operation.;-;Results: There was a significant difference between benign and malignant tumours in the values of ADC, FA and SWV (p = 0.032, p = 0.011 and p < 0.0001); a significant difference in the values was also found between pleomorphic adenoma and malignant tumour (p = 0.0012, p < 0.0001 and p = 0.0002). The diagnosis cut-off points between benign and malignant tumours for ADC, FA and SWV were 1.02 x 10(-3) mm(2) s(-1), 0.24 and 2.76 m s(-1), respectively; the sensitivity for ADC, FA and SWV was 87.50, 62.50 and 68.75%; the specificity was 45.71, 82.86 and 97.14%. Analysis of the combination of the three parameters increased the sensitivity, specificity, Youden index and area under the ROC curve compared with analysis of each parameter alone for distinguishing benign and malignant tumours.;-;Conclusions: The diagnostic value of the combination of the three parameters for distinguishing benign and malignant parotid tumours is the best; SWV is the preferred indicator. Parameters of DTI and ARFI may reflect the histological characteristics of parotid tumours and predict benignancy and malignancy and could provide quantitative information about the tumour. Combination of DTI with ARFI imaging had obvious advantage for the diagnosis of parotid tumours than each alone.
Quantitative tissue velocity imaging evaluation of ventricular function in obstructive sleep apnoea-hypopnoea syndrome in children
Mu, H;Liu, JJ;Gong, KB;Li, DM;Zhang, JJ
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 2015年 42卷6期 页码:602-608
AMBULATORY BLOOD-PRESSURE; HEART-FAILURE; DYSFUNCTION
This study aimed to evaluate the changes in left and right ventricular functions of children with obstructive sleep apnoea-hypopnoea syndrome (OSAHS) and to determine the efficacy of surgical treatment for OSAHS in children. The subjects included 20 normal controls and 55 children of OSAHS with adenoid and/or tonsil hypertrophy diagnosed by polysomnography (PSG). We divided the children with OSAHS into the mild group, moderate group and severe group of OSAHS according to the condition of apnoea-hypopnoea index (AHI) and the lowest ofoxygen saturation (LSaO(2)). In mitral annulus,the Va values were increased in moderate and severe OSAHS (P>0.05), and the Ve/Va values were decreased in the severe group (P>0.05), while the Vs values were not changed (P>0.05). In tricuspid annulus, the Vs values were decreased in moderate and severe OSAHS (P<0.05), while the Ve, Va and Ve/Va values were not changed (P>0.05). Six months after adenoidectomy and/or tonsillectomy, mitral Va values were decreased and tricuspid Vs values were increased significantly (P<0.05), and AHI was decreased and LSaO(2) was significantly increased (P<0.05). Compared with the control group, no difference was found in the above parameters (P>0.05). The left ventricular diastolic function and the right ventricular systolic function of children with moderate to severe OSAHS are decreased to varying degrees at an early stage. Mitral annular and tricuspid annular velocity detected by quantitative tissue velocity imaging (QTVI) could sensitively reflect the early changes of left and right ventricular function. Adenoidectomy and/or tonsillectomy wereeffective methods to treat childhood OSAHS, which could reverse myocardial dysfunction.
Joint cavity injection combined with manual reduction and stabilization splint treatment of anterior disc displacement
Liu, JJ;Mu, H;Wang, ZF;Lan, J;Zhang, SZ;Long, X;Zhang, DS
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 2015年 8卷4期 页码:5943-5948
TEMPOROMANDIBULAR-JOINT; SODIUM HYALURONATE; ARTHROCENTESIS; OSTEOARTHRITIS; DISORDERS; MOBILITY; MRI
Aim: This study aimed to compare the clinical efficacy of upper and lower joint cavity treatment (UJCT vs. LJCT) in patients with anterior disc displacement without reduction (ADDw/oR) of temporomandibular joint (TMJ). Material and methods: A total of 56 patients with unilateral ADDw/oR were randomly divided into two groups: UJCT group and LJCT group. Manual reduction was done in all the patients after joint cavity rejection of sodium hyaluronate. Then, they were treated with stabilization splint for one or two months. At last, Friction index was calculated to evaluate the therapeutic efficacy at 6 to 12 months follow-up. Results: The maximal mouth-opening degrees in the both groups increased significantly when compared with pre-treatment group (P < 0.01), and the Friction index decreased significantly when compared with pre-treatment group (P < 0.01); In LJCT group, the degrees of maximal mouth-opening increased significantly as compared to UJCT group (P < 0.05), and Friction index were also markedly lower than that in UJCT group (P < 0.05). Conclusion: In the patients with ADDw/oR of TMJ, the clinical efficacy of LJCT is superior to that of UJCT, especially in the TMJ pain relief, mouth-opening degree and mandibular movement improvement.
Inhibition of autophagy augments chemotherapy in human salivary adenoid cystic carcinoma
Jiang, LC;Huang, SY;Zhang, DS;Zhang, B;Li, KY;Li, WG;Zhang, SZ;Zhang, WD;Zheng, PH
JOURNAL OF ORAL PATHOLOGY & MEDICINE 2014年 43卷4期 页码:265-272
HUMAN CANCER-CELLS; INDUCED APOPTOSIS; GROWTH-CONTROL; P53; THERAPY; DEATH; PATHWAYS; SUPPRESSION; MECHANISMS; STRESS
Although cisplatin (DDP)-based adjuvant chemotherapy is widely used in the treatment of salivary adenoid cystic carcinoma (SACC), SACCs have developed resistance to cisplatin, resulting in chemotherapy failure. Autophagy serves as a critical adaptive response, which was increased in tumor cells in chemotherapy. However, the function of autophagy is not clear in SACC. In this study, apoptosis induced by DDP in SACC high metastatic cell line (ACC-M) was revealed using MTT assay, flow cytometry, and caspase-3 immunoblotting. The autophagy activation induced by DDP treatment was measured by transmission electron microscopy, green fluorescent protein-light chain 3 plasmid transfection LC3 immunoblotting and p62 immunoblotting. 3-methyladenine (3-MA) or small interference RNA targeting beclin 1 (beclin 1 siRNA) inhibited autophagy and significantly enhanced DDP-induced apoptosis. ACC-M xenografts in nude mice further verified the synergistic effect of DDP and 3-MA. In conclusion, autophagy activation was caused to protect cancer cells from DDP-induced apoptosis and autophagy inhibition could be a promising strategy for adjuvant chemotherapy in SACC.
Temsirolimus, the mTOR inhibitor, induces autophagy in adenoid cystic carcinoma: In vitro and in vivo
Liu, WL;Huang, SY;Chen, ZW;Wang, HC;Wu, HW;Zhang, DS
PATHOLOGY RESEARCH AND PRACTICE 2014年 210卷11期 页码:764-769
CELL-DEATH; A549 CELLS; BECLIN 1; CANCER; EXPRESSION; THERAPY; PATHWAY; PROTEIN; TARGET; HEAD
Temsirolimus acts as a mammalian target of rapamycin(mTOR)-dependent autophagic inhibitor. In order to clarify its effects and mechanisms on human salivary adenoid cystic carcinoma (ACC), we examined whether temsirolimus induced autophagy as the mTOR inhibitor in ACC, both in vitro and in vivo. In this study, MTT assay showed that the inhibition effect of temsirolimus assumed an obvious dose-response relationship on ACC-M cells, and the 50% inhibitory concentration (IC50) approached 20 mu mol/1; numerous autophagosomes were observed by the transmission electron microscopy (TEM) in temsirolimus treatment groups; notably, expression of LC3 and Beclin1 was significantly up-regulated by temsirolimus. More importantly, the xenograft model provided further evidence of temsirolimus-induced autophagy in vivo by inhibiting mTOR activation as well as up-regulation the expression of Beclin1. These results suggest that temsirolimus could act as an mTOR inhibitor to induce autophagy in adenoid cystic carcinoma both in vitro and in vivo. (C) 2014 Elsevier GmbH. All rights reserved.
Expression of autophagy and ER stress-related proteins in primary salivary adenoid cystic carcinoma
Jiang, LC;Huang, SY;Li, WG;Zhang, DS;Zhang, SZ;Zhang, WD;Zheng, PH;Chen, ZW
PATHOLOGY RESEARCH AND PRACTICE 2012年 208卷11期 页码:635-641
ENDOPLASMIC-RETICULUM STRESS; GLUCOSE-REGULATED PROTEINS; CLINICAL-SIGNIFICANCE; MOLECULAR-MECHANISMS; FAVORABLE PROGNOSIS; LUNG-CANCER; BECLIN 1; GRP78; SURVIVAL; INDUCTION
Autophagy is the endogenous cellular pathway that facilitates cellular survival by maintaining energy homeostasis and macromolecular synthesis during cellular stress and nutrient deprivation. Endoplasmic reticulum (ER) stress is the process in which disruption of these physiological functions leads to an accumulation of unfolded proteins and induces the unfolded protein response (UPR). ER stress and autophagy are involved in human cancer. We investigated the expression of autophagic proteins (LC3 and beclin 1) and ER stress-related protein (GRP78) in head and neck adenoid cystic carcinoma tissue. Tissue samples from 79 cases of head and neck adenoid cystic carcinoma tissue were utilized for immunohistochemistry. LC3 expression was significantly correlated with lymph node involvement (P = .016) and TNM (P = .021). Beclin 1 expression was significantly correlated with the histological growth pattern (P = .002), the histological grade (P = .000), and longer survival (P = .000). GRP78 expression was significantly correlated with the histological growth pattern (P = .019), the histological grade (P = .019), and longer survival (P = .001). LC3 expression was positively correlated with beclin 1 expression (P = .000): LC3 and beclin 1 expressions were positively correlated with GRP78 expression respectively (P = .035) (P = .008). Our study describes the expression of LC3, beclin 1, and GRP78 in adenoid cystic carcinoma and its relationship with clinicopathologic factors and overall survival. These results suggest that LC3, beclin 1, and GRP78 may play an important role in the tumorigenesis of adenoid cystic carcinoma, and that beclin 1 and GRP78 may serve as new prognostic indicators for the outcome of patients with adenoid cystic carcinoma. (c) 2012 Elsevier GmbH. All rights reserved.
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