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Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-kappa B-mediated by RhoA/ROCK signalling pathway
Wang, QL; Shen, ZW; Qi, GH; Zhao, YF; Zhang, HG; Wang, R
CELL ADHESION & MIGRATION 2020年 14卷1期 页码:42-56
GLYCATION END-PRODUCTS; RENIN-ANGIOTENSIN SYSTEM; ACUTE LUNG INJURY; DIABETIC-NEPHROPATHY; PATHOPHYSIOLOGY; COMPLICATIONS; PATHOGENESIS; ADHESION
Advanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed that stimulation with AGE could significantly activate RhoA/NF-kappa B pathway. Results showed thymol could markedly suppress inflammatory responses, cell apoptosis and disordered cytoskeleton. Also thymol restored the expression of podocin, restrained migration capacity. Western blot analysis indicated that it could restore the expression of RhoA, ROCK and vimentin, nephrin, podocin and p65 and I kappa B alpha phosphorylation. Moreover, si-RhoA also suppressed the expression of pro-inflammatory cytokines, ROCK, and vimentin and the phosphorylation of p65 and I kappa B alpha. In conclusion, thymol inhibits AGE-induced cell injury in HPCs by suppressing the RhoA-NF-kappa B pathway and may be apromising therapeutic agent.
Up-regulation of TIF1 gamma by valproic acid inhibits the epithelial mesenchymal transition in prostate carcinoma through TGF-beta/Smad signaling pathway
Qi, GH; Lu, GL; Yu, JG; Zhao, YF; Wang, CH; Zhang, HG; Xia, QH
EUROPEAN JOURNAL OF PHARMACOLOGY 2019年 860卷
HISTONE DEACETYLASE INHIBITOR; BREAST-CANCER CELLS; PROMOTES; SMAD4; EXPRESSION; UBIQUITINATION; HEMATOPOIESIS; TRICHOSTATIN; METASTASIS; REPRESSION
Valproic acid (VPA), one of the histone deacetylase inhibitors, can suppress prostate cancer (PCa) cells epithelial mesenchymal transition (EMT). Transcriptional intermediary factor 1 gamma (TIF1 gamma) which is a vital protein molecule that possesses ubiquitination enzyme activity, can mediate TGF-beta induced EMT. We aimed to investigate the detailed mechanism between VPA and EMT occurrence in PCa cells to clarify the potential mechanism of TIF1 gamma involved. In our vitro experiments, we first investigated the effect of VPA on the expression TIF1 gamma. After TIF1 gamma was knockdown or overexpressed by related lentivirus, EMT of PCa cells were assessed. When TIF1 gamma knockdown or overexpress stable cell line were established, cells were treated with additional VPA, EMT index were detected and functional experiments were also conducted to confirm whether VPA inhibited EMT of PCa cells via TIF1 gamma. The mono-ubiquitination of Smad4 was analyzed simultaneously. In vivo, mice were facilitated with PC3 cells or TIF1 gamma related knockdown or overexpress virus transfected PC3 cells with or without VPA administration. Results showed that in vitro VPA can increase the expression of TIF1 gamma and also induce the increase expression of E-cadherin, and the decrease of N-cadherin and vimentin. Knocking down of TIF1 gamma can effectively block the effect of VPA on EMT and metastasis while overexpression of TIF1 gamma can strengthen its role. In vivo VPA also showed its anti-growth effect including tumor growth and EMT mediated by TIF1 gamma coincide with in vitro experiments. In conclusion, VPA inhibits the EMT in PCa cells via up-regulating the expression of TIF1 gamma and the mono-ubiquitination Smad4. VPA could serve as a promising agent in PCa treatment, with new strategies based on its diverse effects on posttranscriptional regulation.
头颈癌术后病人出院准备度现状及影响因素分析
赵延芳;侯亚丽;黄智龙;辛翠翠;王敏;胡艳;
护理研究 2023年 37卷06期 页码:993-999 影响因子:1.123
头颈癌;;出院准备度;;影响因素;;护理
目的:调查头颈癌术后病人出院准备度现状,并分析其影响因素。方法:采用便利抽样法,选取2020年12月—2022年1月就诊于山东第一医科大学附属省立医院及山东省耳鼻喉医院耳鼻喉病房及口腔病房的353例头颈癌术后病人为研究对象。采用一般资料调查表、出院准备度量表(RHDS)、出院指导质量量表(QDTS)、华盛顿大学生存质量问卷(UW-QOL)、社会支持量表(SSRS)进行调查,并分析头颈癌术后病人出院准备度的影响因素。结果:头颈癌术后病人RHDS总分为(156.91±15.88)分,回归分析结果显示,出院前病人实际获得的内容、出院指导技巧、年龄、吞咽、文化程度、客观支持进入回归方程,共解释变异的47.9%。结论:头颈癌病人出院准备度处于中等水平,医护人员应增加院内病人实际获得的知识,并依据病人年龄、文化程度、吞咽状况、客观支持情况实施个性化的健康宣教,以提高病人出院准备度。
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