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MiR-135b-5p and MiR-499a-3p Promote Cell Proliferation and Migration in Atherosclerosis by Directly Targeting MEF2C
Xu, ZL;Han, YM;Liu, JY;Jiang, F;Hu, HL;Wang, Y;Liu, QJ;Gong, YQ;Li, X
SCIENTIFIC REPORTS 2015年 5卷
CORONARY-ARTERY-DISEASE; CIRCULATING MICRORNAS; ENDOTHELIAL-CELLS; EXPRESSION; INFLAMMATION; REGULATOR; DIFFERENTIATION; MICROPARTICLES; ANGIOGENESIS; STATINS
Proliferation and migration of endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) are critical processes involved in atherosclerosis. Recent studies have revealed that microRNAs (miRNAs) can be detected in circulating blood with a stable form and the expression profiles differ in many cellular processes associated with coronary artery disease (CAD). However, little is known about their role, especially serum-derived miRNAs, in ECs and VSMCs phenotype modulation during atherosclerosis. We compared the miRNA expressions in serum samples from 13 atherosclerotic CAD patients and 5 healthy control subjects and identified 36 differentially expressed miRNAs. The expression of selected miRNAs (miR-135b-5p and miR-499a-3p) was further validated in 137 serum samples. Interestingly, miR-135b-5p and miR-499a-3p directly regulated a common target gene: myocyte enhancer factor 2C (MEF2C) which plays an important role in modulating cell phenotype of cardiovascular systems. Furthermore, our results indicated that the 2 elevated miRNAs could jointly promote ECs and VSMCs proliferation and migration by repressing MEF2C expression. Together, our findings demonstrated a serum-based miRNA expression profile for atherosclerotic CAD patients, potentially revealing a previously undocumented mechanism for cell proliferation and migration mediated by miR-135b-5p and miR-499a-3p, and might provide novel insights into the role of circulating miRNAs in atherosclerosis pathogenesis.
Adipose tissue hypoxia and low-grade inflammation: a possible mechanism for ethanol-related glucose intolerance?
He, Z;Li, M;Zheng, DM;Chen, Q;Liu, WW;Feng, L
BRITISH JOURNAL OF NUTRITION 2015年 113卷9期 页码:1355-1364
TUMOR-NECROSIS-FACTOR; BODY-MASS INDEX; NF-KAPPA-B; INSULIN-RESISTANCE; ALCOHOL-CONSUMPTION; METABOLIC SYNDROME; ABDOMINAL OBESITY; DIABETES-MELLITUS; ENDOCRINE ORGAN; RISK-FACTORS
The exact mechanism of ethanol's effects on glucose tolerance has not been well determined. The present study focuses for the first time on hypoxia and low-grade inflammation in adipose tissue (AT). In the in vivo experiments, twenty-four male Wistar rats were randomly allocated into control and ethanol feeding groups. Ethanol-treated rats received edible ethanol once a day at a total dosage of 5g/kg per d, and the controls received distilled water. Ethanol volumes were adjusted every week. At the end of 8 weeks, we carried out an oral glucose tolerance test. Blood and AT were collected for measuring hypoxia-inducible factor-1 alpha (HIF-1 alpha), GLUT1, TNF-alpha, IL-6, leptin and vascular endothelial growth factor (VEGF). In the in vitro experiments, differentiated OP9 adipocytes were incubated with 100mm of ethanol for 48h; the media and cells were then collected for measuring HIF-1 alpha, GLUT1, TNF-alpha and IL-6. The results showed that long-term ethanol consumption impaired glucose tolerance in rats. Ethanol consumption had little influence on body weight, but both epididymal and perirenal AT were markedly enlarged in the ethanol-treated rats as compared to the controls. Visceral adipose tissue (VAT) had accumulated, and the protein levels of HIF-1 alpha and GLUT1, the indicators of hypoxia in rat epididymal AT and OP9 adipocytes, were elevated. Secondary to the AT hypoxia, the levels of inflammation-related adipokines, such as TNF-alpha, IL-6, leptin and VEGF, were increased. Based on these findings, we conclude that VAT hypoxia and low-grade inflammation might be a new mechanism in the treatment of ethanol-related diabetes.
Diagnostic Efficiency of Low-Dose CT Angiography Compared With Conventional Angiography in Peripheral Arterial Occlusions
Duan, YH;Wang, XM;Yang, XC;Wu, DW;Cheng, ZP;Wu, LB
AMERICAN JOURNAL OF ROENTGENOLOGY 2013年 201卷6期 页码:W906-W914
DIGITAL-SUBTRACTION-ANGIOGRAPHY; COMPUTED-TOMOGRAPHY ANGIOGRAPHY; LOWER-EXTREMITY ARTERIES; IMAGE QUALITY; LOW-KILOVOLTAGE; MR-ANGIOGRAPHY; CHEST CT; MULTIDETECTOR; DISEASE; ENHANCEMENT
OBJECTIVE. The purpose of this study was to assess the diagnostic efficiency and radiation dose of peripheral arterial CT angiography (CTA) performed at a low tube voltage of 70 kV in comparison with conventional angiography.;-;SUBJECTS AND METHODS. Thirty consecutive patients (body mass index <= 25 kg/m(2)) with known or suspected peripheral arterial occlusion diseases underwent both CTA at a low tube voltage of 70 kV and conventional angiography. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of peripheral arterial CTA were evaluated. The radiation dose was recorded.;-;RESULTS. Diagnostic CTA images were obtained in all patients. CTA allowed accurate identification, characterization, and measurement of all peripheral arterial occlusive diseases. In conventional angiography, 360 diseased segments were found among the 810 segments evaluated. The sensitivity, specificity, PPV, NPV, and accuracy of CTA were 100% (95% CI, 98.81-100%), 93.5% (90.96-95.36%), 90.86% (87.38-93.45%), 100% (99.17-100%), and 96.05% (94.48-97.19%), respectively, with a kappa value of 0.92 (excellent agreement). The mean CT dose index was 3.71 +/- 0.8 mGy, and the dose-length product was 446.6 +/- 35.7 mGy x cm. The effective dose was 1.94 +/- 0.21 mSv for CTA and 4.41 +/- 0.64 mSv for conventional angiography.;-;CONCLUSION. CTA of peripheral arteries with a low tube voltage of 70 kV provides reliable information and serves as a rapidly performed and easily available "one-stop-shop" imaging modality in the diagnosis of peripheral arterial occlusion diseases.
Carto三维电标测系统引导下儿童室性早搏射频消融临床疗效观察
刘继英;崔连群;周嘉;姜佳男
山东医药 2018年 58卷29期 页码:60-62 影响因子:0.805
室性早搏;;射频消融术;;CARTO三维电标测系统;;治疗结果;;儿童;;
目的 探讨Carto三维电标测系统引导下儿童室性早搏射频消融的临床疗效.方法 选取儿童室性早搏31例,在局部麻下穿刺右侧股静脉并置入标测电极及消融导管,在CARTO指导下实时重建心腔三维解剖图形,通过激动标测或者起搏标测观察激动顺序颜色的变化,标测出异常激动点,行Carto三维电标测系统引导下射频消融治疗.结果 31例患儿中,麻醉后未行手术治疗室性早搏即消失1例,射频消融成功27例,有效2例,复发1例,射频消融成功率为90.32%.复发1例经第2次射频消融成功.术中无严重并发症发生,术后均康复出院.结论 Carto三维电标测系统引导下射频消融儿童室性早搏安全有效.
自制覆膜支架抢救急性冠脉穿孔的护理配合
吴玉丽;宋蕾;杨显存;孙有伟
医学影像学杂志 2018年 28卷8期 页码:1401-1403 影响因子:0.81
放射介入学,覆膜支架;;急性冠脉穿孔;;护理配合;;
目的 1例3M敷贴自制覆膜支架抢救急性冠脉穿孔的护理配合,旨在总结经验和提高预防及处理的能力.方法 护士与医生密切配合,用无菌3M敷贴,根据支架的大小,剪裁合适尺寸,缠绕在支架外1.5 ~2圈,然后支架塑性,随即将自制的覆膜支架送至冠状动脉穿孔处,顺利通过病变,定位成功后释放覆膜支架.结果 经反复造影证实,支架贴壁良好,冠脉穿孔处完全被自制覆膜支架覆盖且整个狭窄段血管扩张良好,同时解除了患者狭窄最重的责任血管,患者胸闷、心前区不适等症状缓解,血压、心率回升,转危为安,安返病房.于术后5天出院.术后随访1年,患者无胸痛胸闷等症状.结论 3M敷贴自制覆膜支架的制作和植入操作相对简单、用物经济实惠易取,加之医护默契配合,取得了满意的效果.
主动脉瓣置换术后起搏器囊袋血肿使用高频电刀的安全护理1例
宋蕾;李敏
医学影像学杂志 2017年 27卷11期 页码:2230-2232 影响因子:0.81
主动脉瓣置换术;;永久性心脏起搏器植入;;囊袋血肿;;高频电刀;;安全护理;;
目的 探讨心脏瓣膜置换术后长期服用华法令抗凝的患者,行永久起搏器安装术后6h出现起搏器囊袋血肿使用高频电刀止血的安全护理.方法 通过术前对患者进行安全性评估,制定有针对性的护理计划,采取各项有针对性的护理措施,术中严密监护和熟练配合高频电刀的使用;术后随访等护理措施,有效的保证患者的安全,防止不安全因素及意外的发生.结果 本次手术在使用高频电刀过程中患者病情无异常变化,止血效果良好.术后1、3、6个月患者于心血管门诊随访,患者囊袋情况良好,起搏、感知功能正常,程控参数无明显变化.结论 主动脉瓣置换术后长期服用华法令抗凝的患者,植入永久起搏器,安全护理下电刀使用安全、有效、可靠,值得推广.
桡动脉充气止血绑带在冠状动脉介入术中的效果评价
丁蕊;陈士巧;宋蕾
医学影像学杂志 2014年 24卷8期 页码:1311-1314页,共4页 影响因子:0.81
桡动脉充气止血绑带;经皮冠状动脉介入术;效果评价
目的:探讨桡动脉充气止血绑带在桡动脉途径经皮冠状动脉介入术(PCI)后的有效性及安全性。方法选取2012年于山东大学附属省立医院接受经皮冠状动脉介入术患者共1069例。随机分为两组,实验组使用桡动脉充气止血绑带压迫止血,对照组使用常规的绷带压迫止血。观察比较两组止血时间、制动时间、患者压迫点局部情况、术后舒适度及并发症发生率。结果两组患者年龄、性别、基础疾病等方面比较差别无统计学意义( P >0.05)。实验组止血时间和制动时间均短于对照组,差异有统计学意义(P <0.05),并发症发生率少于对照组(P <0.05)手部肿胀程度(轻度、中度、重度)发生率均少于对照组( P <0.05),两组患者满意度比较有统计学差异( P <0.05)。结论经桡动脉途径PCI术后应用桡动脉充气止血绑带安全有效,止血时间及制动时间短,并发症少,同时给予专业护理干预,提高了患者舒适度与满意度,值得临床推广。
TIPSS治疗肝硬化门脉高压症的术后并发症及护理
丁蕊;周嘉;钟晶;杨显存
医学影像学杂志 2010年 20卷8期 页码:1142-1145页,共4页 影响因子:0.81
经颈静脉,肝内门体支架分流术;肝硬变,门脉高压症;并发症;护理
目的:探讨经颈静脉肝内门体支架分流术(transjugular intrahepatic portosystemic stent-shunt, Tipss)治疗肝硬化门脉高压症的并发症及护理.方法:对我院50例肝硬化Child-Push分级B、C级患者分别给予Tipss术,术中、术后严密观察病情变化、手术进程及各种并发症的发生情况,并针对不同情况,给予细致的护理观察,总结观察结果.结果:50例患者术后平均门静脉压力下降20cm H2O,术后肝硬化门脉高压患者的腹水或上消化道出血两大并发症得到不同程度的缓解;术后1月内,除1例1周后支架即闭塞,1例术后即并发严重肝性脑病外,未见其他并发症的发生,远期并发症主要以反复的肝性脑病及支架闭塞为主,其他肝静脉闭塞、肝性神经病变较少见;术后1月复查肝功,平均Child积分变化不大.结论:Tipss是治疗肝硬化合并门脉高压症有效方法,术后门脉压力缓解明显,但术后支架狭窄与肝性脑病高发,通过加强护理可降低其发生率,但肝性脑病仍处在较高的水平,是Tipss开展的又一难题.
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