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Narrow band imaging-assisted transurethral resection reduces the recurrence risk of non-muscle invasive bladder cancer: A systematic review and meta-analysis
Kang, WT;Cui, ZL;Chen, QQ;Zhang, D;Zhang, HY;Jin, XB
ONCOTARGET 2017年 8卷14期 页码:23880-23890 影响因子:5.008
TRANSITIONAL-CELL-CARCINOMA; WHITE-LIGHT CYSTOSCOPY; HEXAMINOLEVULINATE FLUORESCENCE CYSTOSCOPY; 5-AMINOLEVULINIC ACID FLUORESCENCE; PHOTODYNAMIC DIAGNOSIS; PHASE-III; FOLLOW-UP; 7 EORTC; TUMORS; PROGRESSION
Context: Compared with white light imaging (WLI) cystoscopy, narrow band imaging (NBI) cystoscopy could increase the visualization and detection of bladder cancer (BC) at the time of transurethral resection (TUR). NBI cystoscopy could increase the detection of BC, but it remains unclear whether narrow band imaging-assisted transurethral resection (NBI-TUR) could reduce the recurrence risk of nonmuscle invasive bladder cancer (NMIBC). Several randomized clinical trials (RCTs) have recently tested the efficacy of NBI-TUR for NMIBC.;-;Objective: To perform a systematic review and meta-analysis of RCTs and evaluate the efficacy of NBI-TUR for NMIBC compared with white light imaging-assisted transurethral resection (WLI-TUR). The end point was recurrence risk.;-;Evidence acquisition: A systematic review of PubMed, Medline, Ovid, Embase, Cochrane and Web of Science was performed in February 2016 and updated in July 2016.;-;Evidence synthesis: Overall, six (n = 1084) of 278 trials were included. Three trials performed narrow band imaging-assisted electro-transurethral resection (NBIETUR), and two trials performed narrow band imaging-associated bipolar plasma vaporization (NBI-BPV). The last trial performed narrow band imaging-associated holmium laser resection (NBI-HLR). Statistical analysis was performed using Review Manager software (RevMan v.5.3; The Nordic Cochrane Center, Copenhagen, Denmark). The recurrence risk was compared by calculating risk ratios (RRs) with 95% confidence interval (CIs). Risk ratios with 95% CIs were calculated to compare 3-mo, 1-yr, and 2-yr survival rates. NBI-TUR was associated with improvements in the 3-mo recurrence risk (RR: 0.39; 95% CI, 0.26-0.60; p < 0.0001), 1-yr recurrence risk (RR: 0.52; 95% CI, 0.40-0.67; p < 0.00001) and 2-yr recurrence risk (RR: 0.60; 95% CI, 0.42-0.85; p = 0.004) compared with WLI-TUR.;-;Conclusions: Compared with WLI-TUR, NBI-TUR can reduce the recurrence risk of NMIBC. The results of this review will facilitate the appropriate application of NBI in NMIBC.
Sodium Ferulate Protects against Angiotensin II-Induced Cardiac Hypertrophy in Mice by Regulating the MAPK/ERK and JNK Pathways
Hu, B;Song, JT;Ji, XF;Liu, ZQ;Cong, ML;Liu, DX
BIOMED RESEARCH INTERNATIONAL 2017年
INDUCED APOPTOSIS; ANG-II; STRESS; KINASE; BETA; RATS; INHIBITION; EXPRESSION; FIBROSIS; FAILURE
Background and Objective. It has been reported that sodium ferulate (SF) has hematopoietic function against anemia and immune regulation, inflammatory reaction inhibition, inhibition of tumor cell proliferation, cardiovascular and cerebrovascular protection, and other functions. Thus, this study aimed to investigate the effects of SF on angiotensin II- (AngII-) induced cardiac hypertrophy in mice through the MAPK/ERK and JNK signaling pathways. Methods. Seventy-two male C57BL/6J mice were selected and divided into 6 groups: control group, PBS group, model group (AngII), model + low-dose SF group (AngII + 10 mg/kg SF), model + high-dose SF group (AngII + 40 mg/kg SF), and model + high-dose SF + agonist group (AngII + 40 mg/kg SCU + 10 mg/kg TBHQ). After 7 d/14 d/28 days of treatments, the changes of blood pressure and heart rates of mice were compared. The morphology of myocardial tissue and the apoptosis rate of myocardial cells were observed. The mRNA and protein expressions of atrial natriuretic peptide (ANP), transforming growth factor-beta (TGF-beta), collagen III (Col III), and MAPK/ERK and JNK pathway-related proteins were detected after 28 days of treatments. Results. SF improved the mice's cardiac abnormality and decreased the apoptosis rate of myocardial cells in a time-and dose-dependent manner (all p < 0.05). MAPK/ERK pathway activator inhibited the protective effect of SF inmyocardial tissue of mice (p < 0.05). SF could inhibit the expression of p-ERK, p-p38 MAPK, and p-JNK and regulate the expressions of ANP, TGF-beta, and Col III (all Rho < 0.05). Conclusion. Our findings provide evidence that SF could protect against AngII-induced cardiac hypertrophy in mice by downregulating the MAPK/ERK and JNK pathways.
Association of Genetic Polymorphisms on VEGFA and VEGFR2 With Risk of Coronary Heart Disease
Liu, DX;Song, JT;Ji, XF;Liu, ZQ;Cong, ML;Hu, B
MEDICINE 2016年 95卷19期
ENDOTHELIAL GROWTH-FACTOR; ARTERY-DISEASE; CARDIOVASCULAR-DISEASE; SUSCEPTIBILITY; HYPERTENSION; POPULATION; STROKE; METAANALYSIS; ANGIOGENESIS; EXPRESSION
Coronary heart disease (CHD) is a cardiovascular disease which is contributed by abnormal neovascularization. VEGFA (vascular endothelial growth factor A) and VEGFR2 (vascular endothelial growth factor receptor 2) have been revealed to be involved in the pathological angiogenesis. This study was intended to confirm whether single nucleotide polymorphisms (SNPs) of VEGFA and VEGFR2 were associated with CHD in a Chinese population, considering pathological features and living habits of CHD patients.Peripheral blood samples were collected from 810 CHD patients and 805 healthy individuals. Six tag SNPs within VEGFA and VEGFR2 were obtained from HapMap Database. Genotyping of SNPs was performed using SNapShot method (Applied Biosystems, Foster, CA). Odd ratios (ORs) and their 95% confidence intervals (95% CIs) were calculated to evaluate the association between SNPs and CHD risk.Under the allelic model, 6 SNPs of VEGFA and VEGFR2 were remarkably associated with the susceptibility to CHD. Genotype CT of rs3025039, TT of rs2305948, and AA of rs1873077 were associated with a reduced risk of CHD when smoking, alcohol intake and diabetes were considered, while homozygote GG of rs1570360 might elevate the susceptibility to CHD (all P<0.05) for patients who were addicted to smoking or those with hypertension. All of the combined effects of rs699947 (CC/CA) and rs2305948 (TT), rs3025039 (TT) and rs2305948 (TT), rs3025039 (CT) and rs1870377 (AA) had positive effects on the risk of CHD, respectively (all P<0.05). By contrast, the synthetic effects of rs69947 (CA/AA) and rs1870377 (TA), rs699947 (CA) and rs7667298 (GG), rs699947 (AA) and rs7667298 (GG), rs1570360 (GG) and rs2305948 (TT), as well as rs1570360 (GG) and rs1870377 (AA) all exhibited adverse effects on the risk of CHD, respectively (all P<0.05).Six polymorphisms in VEGFA and VEGFR2 may have substantial influence on the susceptibility to CHD in a Han Chinese population. Prospective cohort studies should be further designed to confirm the above conclusions.
Complete mitochondrial genome sequence of the heart failure model of cardiomyopathic Syrian hamster (Mesocricetus auratus)
Hu, B;Liu, DX;Zhang, YQ;Song, JT;Ji, XF;Hou, ZQ;Zhang, ZH
MITOCHONDRIAL DNA PART A 2016年 27卷3期 页码:2182-2183
MYOCARDIAL-CONTRACTILITY
In this study we sequenced the complete mitochondrial genome sequencing of a heart failure model of cardiomyopathic Syrian hamster (Mesocricetus auratus) for the first time. The total length of the mitogenome was 16,267 bp. It harbored 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 non-coding control region.
Delivery of a Calcium Channel Blocker Through Buccal Mucosa from Matrix Type Mucoadhesive Films for Improved Cardiac Drug Delivery
Liu, DX;Ji, XF;Song, JT;Liu, ZQ;Hu, B
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING 2015年 5卷7期 页码:586-591
POLYMERS; RELEASE; HYDROCHLORIDE; MECHANISMS; SYSTEMS; VIVO
To improve the bioavailability of verapamil hydrochloride (calcium channel blocker used in several cardiac diseases like angina, arrhythmia, supraventricular tachycardias) by circumventing the hepatic first pass effect, the mucoadhesive buccal films were prepared. Mucoadhesive buccal films were prepared by solvent casting technique using hydroxy ethylcellulose, sodium carboxymethylcellulose, carbopol-972P and Eudragit RL-100. Films were evaluated for their weight, thickness, drug content uniformity, surface pH, swelling index, in vitro residence time, folding endurance, in vitro release and ex vivo permeation studies. All the films showed high drug content, good swelling and in vitro residence time. And controlled release over more than 6 h. After 6 h the in vitro release was found to be in the range of 70.24 to 94.56%. It was concluded that the films containing 50 mg verapamil in 5% w/v hydroxy ethylcellulose and 1.5% w/v sodium carboxymethylcellulose exhibited satisfactory swelling, an optimum residence time and promising drug release thus proved to be potential candidate for the development of buccal films for cardiac diseases like arrythmia.
Penile erectile dysfunction after brachial plexus root avulsion injury in rats
Fu, G;Qin, BG;Jiang, L;Huang, XJ;Lu, QS;Zhang, DC;Liu, XL;Zhu, JK;Zheng, JW;Li, XJ;Gu, LQ
NEURAL REGENERATION RESEARCH 2014年 9卷20期 页码:1839-1843
BROWN-SEQUARD SYNDROME; NITRIC-OXIDE; SPINAL-CORD; SEXUAL DYSFUNCTION; TRACTION INJURY; ANIMAL-MODEL; PALSY; REPAIR; SATISFACTION; MECHANISMS
Our previous studies have demonstrated that some male patients suffering from brachial plexus injury, particularly brachial plexus root avulsion, show erectile dysfunction to varying degrees. However, the underlying mechanism remains poorly understood. In this study, we evaluated the erectile function after establishing brachial plexus root avulsion models with or without spinal cord injury in rats. After these models were established, we administered apomorphine (via a subcutaneous injection in the neck) to observe changes in erectile function. Rats subjected to simple brachial plexus root avulsion or those subjected to brachial plexus root avulsion combined with spinal cord injury had significantly fewer erections than those subjected to the sham operation. Expression of neuronal nitric oxide synthase did not change in brachial plexus root avulsion rats. However, neuronal nitric oxide synthase expression was significantly decreased in brachial plexus root avulsion + spinal cord injury rats. These findings suggest that a decrease in neuronal nitric oxide synthase expression in the penis may play a role in erectile dysfunction caused by the combination of brachial plexus root avulsion and spinal cord injury
Prognostic significance of TBX2 expression in non-small cell lung cancer
Hu, B;Mu, HP;Zhang, YQ;Su, CY;Song, JT;Meng, C;Liu, DX
JOURNAL OF MOLECULAR HISTOLOGY 2014年 45卷4期 页码:421-426
AMPLIFICATION; SENESCENCE; REPRESSES; MARKERS; GENES
T-box2 (TBX2) expression has been reported to be related to aggressive tumor features. However, the role of TBX2 in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. So we aimed at investigating the potential role of TBX2 in NSCLC. TBX2 expression was evaluated by qRT-PCR and Western blotting in 50 paired fresh lung cancer tissues as well as immunohistochemistry on 212 paraffin-embedded sections. We showed that the expression level of TBX2 was significantly increased in NSCLC as compared with the adjacent noncancerous tissue. Positive expression level of TBX2 was associated with histological type, lymph node metastasis and distant metastasis. Kaplan-Meier survival curves showed that positive expression level of TBX2 was associated with poor overall survival (OS) and progression-free survival of NSCLC patients. Results showed that TBX2 positivity was an independent prognostic factor for OS (HR 1.87, 95 % CI 1.004-3.153, p = 0.012). On the basis of these results, we suggested that TBX2 protein expression may be an unfavorable independent prognostic parameter for NSCLC.
洛拉曲克对人大肠癌LoVo细胞胸苷酸合酶水平及细胞增殖的影响
田树波;李乐平;靖昌庆
中国现代普通外科进展 2012年 15卷4期 页码:262-265页,共4页 影响因子:0.711
洛拉曲克;胸苷酸合酶;大肠肿瘤;LoVo细胞
目的:研究洛拉曲克体外对人大肠癌LoVo细胞胸苷酸合酶(TS)水平动态变化以及对细胞增殖的影响.方法:采用四甲基偶氮唑蓝(MTT)法检测不同浓度的洛拉曲克对LoVo细胞抑制率的影响,RT-PCR和Western blot法检测TS基因和蛋白的表达变化,流式细胞术检测细胞凋亡率的变化.结果:洛拉曲克对LoVo细胞有明显的抑制作用,其IC.值约为6.31μ mol/L,并可促进细胞凋亡,凋亡率随作用时间的延长而增加.RT-PCR结果显示,在mRNA水平,相同剂量洛拉曲克处理细胞后,随着时间的延长,TS/GAPDH比值与对照组相比,有持续升高的趋势,但是只有48 h和60 h相对于未处理组差异有统计学意义.相同剂量的洛拉曲克处理细胞后,随着时间的延长,其TS蛋白的表达水平亦有持续升高的趋势.结论:洛拉曲克可以明显促进大肠癌细胞的凋亡并可产生TS诱导现象,术后以此可以更好地指导临床用药.
RNA干扰和洛拉曲克对结直肠癌LOVO细胞胸苷酸合成酶表达及细胞增殖的影响
田树波;靖昌庆;李乐平
中华胃肠外科杂志 2012年 15卷11期 页码:1187-1191页,共5页 影响因子:1.388
结直肠肿瘤;RNA干扰;洛拉曲克;胸苷酸合成酶
目的 探讨下调胸苷酸合成酶(TS)基因以及化疗药洛拉曲克对人结直肠癌LOVO细胞胸苷酸合成酶表达水平以及对细胞生长、凋亡的影响.方法 构建针对人TS基因的小分子干扰RNA(siRNA)和阴性对照,转染至LOVO细胞,利用RT-PCR和Western blot技术观察沉默TS基因后其基因和蛋白表达水平的变化,MTT法检测细胞增殖情况;另联合洛拉曲克作用于LOVO细胞,观察两者对LOVO细胞的TS蛋白表达和细胞生长的影响.结果 转染TS siRNA后,LOVO细胞TS mRNA及蛋白的表达水平明显低于阴性对照组,细胞增殖速度亦低于对照组(均P<0.05).TS siRNA联合洛拉曲克组细胞IC50值为(1.46±0.25)μmol/L,而阴性对照组和单用洛拉曲克组的IC50值分别为(6.81±0.31)μmol/L和(6.47±0.43)μmol/L.TS siRNA联合洛拉曲克作用于细胞36 h后,细胞凋亡指数为(62.12±0.89)%,高于单用TS siRNA和洛拉曲克[(21.56±0.67)%和(40.51±0.83)%,均P<0.05].结论 TS siRNA可以下调人LOVO细胞中TS基因和蛋白的表达,使细胞生长速度减慢,凋亡增加,并可以增强洛拉曲克的药物敏感性.
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