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Catalpol protects synaptic proteins from beta-amyloid induced neuron injury and improves cognitive functions in aged rats
Xia, ZM;Wang, FF;Zhou, S;Zhang, R;Wang, FS;Huang, JH;Wu, EX;Zhang, YF;Hu, YE
ONCOTARGET 2017年 8卷41期 页码:69303-69315 影响因子:5.008
TRIAL OBJECT RECOGNITION; ALZHEIMERS-DISEASE; A-BETA; KINASE-C; SYNAPTOPHYSIN MESSAGE; VESICLE TRAFFICKING; PREFRONTAL CORTEX; MEMORY; HIPPOCAMPUS; EXPRESSION
Synapse loss is one of the common factors contributing to cognitive disorders, such as Alzheimer's disease (AD), which is manifested by the impairment of basic cognitive functions including memory processing, perception, problem solving, and language. The current therapies for patients with cognitive disorders are mainly palliative; thus, regimens preventing and/or delaying dementia progression are urgently needed. In this study, we evaluated the effects of catalpol, isolated from traditional Chinese medicine Rehmannia glutinosa, on synaptic plasticity in aged rat models. We found that catalpol markedly improved the cognitive function of aged male Sprague-Dawley rats and simultaneously increased the expression of synaptic proteins (dynamin 1, PSD-95, and synaptophysin) in the cerebral cortex and hippocampus, respectively. In beta-amyloid (A beta) injured primary rat's cortical neuron, catalpol did not increase the viability of neuron but extended the length of microtubule-associated protein 2 (MAP-2) positive neurites and reversed the suppressive effects on expression of synaptic proteins induced by A beta. Additionally, the effects of catalpol on stimulating the growth of MAP-2 positive neurites and the expression of synaptic proteins were diminished by a PKC inhibitor, bisindolylmaleimide I, suggesting that PKC may be implicated in catalpol's function of preventing the neurodegeneration induced by A beta. Altogether, our study indicates that catalpol could be a potential disease-modifying drug for cognitive disorders such as AD.
Intercellular adhesion molecule 1 is a sensitive and diagnostically useful immunohistochemical marker of papillary thyroid cancer (PTC) and of PTC-like nuclear alterations in Hashimoto's thyroiditis
Zhang, K;Ge, SJ;Lin, XY;Lv, BB;Cao, ZX;Li, JM;Xu, JW;Wang, QX
ONCOLOGY LETTERS 2016年 11卷3期 页码:1722-1730
CARCINOMA; EXPRESSION; ICAM-1; GALECTIN-3; CELLS; MICROCARCINOMA; INVASION; FEATURES; RISK; P63
Intercellular adhesion molecule 1 (ICAM-1) is important in the progression of inflammatory responses. Recently, increased levels of ICAM-1 have been reported in a number of types of malignancy. The present study aimed to investigate ICAM-1 expression in papillary thyroid cancer (PTC) and in Hashimoto's thyroiditis (HT) with PTC-like nuclear alterations, and to assess the predictive value of ICAM-1 in thyroid lesions. ICAM-1 expression was retrospectively investigated in 132 consecutive cases of PTC, 72 cases of HT, 10 of follicular cancer, 15 of follicular adenoma, 16 of nodular goiter and 8 samples of normal thyroid tissue using immunohistochemical analyses, and in 42 PTC patients using western blotting. ICAM-1 expression was not detected in normal follicular cells, follicular lesions (adenoma and cancer) and benign nodular hyperplasia, but was frequently overexpressed in PTC cells. ICAM-1 overexpression was associated with extra-thyroidal invasion and lymph node metastasis; no association was found with age, gender, tumor size, multifocality, pathological stage, recurrence or distant metastasis. ICAM-1 expression in HT patients with PTC-like nuclear alterations was significantly higher than that in HT cases with non-PTC-like features. Compared with antibodies against cytokeratin 19, galectin-3 and Hector Battifora mesothelial-1, ICAM-1 was the most sensitive marker for the detection of PTC-like features in HT. These findings demonstrate that ICAM-1 expression is upregulated in PTC and in HT with PTC-like nuclear alterations. This feature may be an important factor in the progression of cancer of the thyroid gland.
Role of F-18-FDG PET/CT in detecting pelvic lymph-node metastases in patients with early-stage uterine cervical cancer: comparison with MRI findings
Lv, K;Guo, HM;Lu, YJ;Wu, ZX;Zhang, K;Han, JK
NUCLEAR MEDICINE COMMUNICATIONS 2014年 35卷12期 页码:1204-1211
POSITRON-EMISSION-TOMOGRAPHY; RADICAL HYSTERECTOMY; TREATMENT RESPONSE; EPITHELIAL TUMORS; FDG-PET; CARCINOMA; IB; IIB; CT
ObjectiveThis study aimed to investigate the applied value of F-18-fluoro-2-dexoxyglucose (F-18-FDG) PET/computed tomography (CT) and MRI in detecting lymph-node metastasis in early-stage cervical cancer.Materials and methodsA retrospective study was performed on 87 early-stage cervical cancer patients evaluated with PET/CT and pelvic MRI before surgery. Histopathological evaluation of lymph nodes served as the diagnostic standard. F-18-FDG PET/CT and MRI images were analyzed and correlated with histopathological findings.ResultsThe overall node-based sensitivity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of PET/CT were 91% (61/67), 78.2% (61/78), 99.4% (1079/1085), and 98% (1140/1163), respectively, which were higher than the corresponding values of MRI, at 37.3% (25/67), 61% (25/41), 96.3% (1080/1122), and 95% (1105/1163) (P<0.034). The difference in diagnostic efficacy for identifying node-based metastases between PET/CT and MRI was significant (PET/CT vs. MRI, 0.719 vs. 0.587, P=0.017). Meanwhile, the overall patient-based sensitivity, PPV, NPV, and accuracy of PET/CT were 100% (34/34), 87.2% (34/39), 100% (48/48), and 94.3% (82/87), respectively, whereas the corresponding MRI values were 44% (15/34), 65% (15/23),74% (45/61), and 69% (60/87) (P<0.04). The difference in diagnostic efficacy for identifying patient-based metastases between PET/CT and MRI was significant (PET/CT vs. MRI, 0.974 vs. 0.705, P<0.001).ConclusionPET/CT has been proven to be valuable in detecting lymph-node metastases. Compared with MRI, PET/CT has higher sensitivity, PPV, NPV, and accuracy in patients with early-stage cervical cancer for detecting lymphatic metastases.
Upregulation of glucosylceramide synthase protein in papillary thyroid carcinoma
Zhang, K;Song, YH;Lin, XY;Wang, QX;Zhang, HW;Xu, JW
CHINESE MEDICAL JOURNAL 2013年 126卷24期 页码:4660-4664
BREAST-CANCER CELLS; MULTIDRUG RESISTENCE 1; DRUG-RESISTANCE; P-GLYCOPROTEIN; EXPRESSION; CERAMIDE; METABOLISM
Background Glucosylceramide synthase (GCS) can reduce ceramide levels and help cells escape ceramide-induced apoptosis, thus leading to multidrug resistance (MDR). However, its expression and clinical significance in thyroid neoplasms still remain unclear. We aimed to elucidate the expression of GCS and explore its correlation with the clinicopathological characteristics in papillary thyroid carcinomas (PTCs).;-;Methods We retrospectively investigated GCS protein expression level in tissue specimens obtained from 108 consecutive PTC patients by immunohistochemistry and Western blotting.;-;Results GCS was weakly positive or negative in normal follicular cells, but it was frequently overexpressed in PTC cells. GCS overexpression was associated with primary tumor size, local infiltration, lymph node metastasis, and local recurrence, but not associated with gender, age, pathological variants, tumor multifocality, tumor stage or distant metastasis. Western blotting also showed that GCS protein levels were much higher in PTCs' tissues than in normal thyroid tissues.;-;Conclusion GCS was upregulated in PTCs and might be an independent factor affecting prognosis.
SPECT study of Chinese schizophrenic patients suggests that cerebral hypoperfusion and laterality exist in different ethnic groups
Li, XB;Tang, JS;Wu, ZX;Zhao, GF;Liu, CY;George, MS
WORLD JOURNAL OF BIOLOGICAL PSYCHIATRY 2005年 6卷2期 页码:98-106
EMISSION-COMPUTED-TOMOGRAPHY; TC-99M HMPAO-SPECT; BLOOD-FLOW; NEGATIVE SYMPTOMS; METABOLIC-ACTIVITY; MEMORY; HYPOFRONTALITY; DYSFUNCTION; DEFICIT; MRI
Hypofrontality is a common finding in schizophrenia in many countries. To date, there have been few studies on Chinese patients with schizophrenia. We thus wondered whether hypofrontality exists in Chinese patients with schizophrenia. We investigated 45 patients with schizophrenia and 21 healthy controls using brain perfusion single photon emission computed tomography (SPECT). Subjects were also administered the Brief Psychiatric Rating Scale (BPRS), Scale for the Assessment of Positive Symptoms (SAPS), Scale for the Assessment of Negative Symptoms (SANS), Halstead-Reitan Neuropsychological Battery (HRNB) and the Wechsler Memory Scale-Revised (WMS-R). Images were analyzed using a semi-quantitative reading and a quantified region of interest analysis. We found that schizophrenic patients showed hypoperfusion in the frontal and temporal lobes and hyperperfusion in the basal ganglia. Schizophrenic patients with both negative and positive symptoms showed asymmetric perfusion in the temporal lobe. Schizophrenic patients with prominent negative symptoms also showed asymmetric perfusion in the prefrontal lobes. Negative symptoms showed a significantly negative correlation with regional cerebral blood flow (rCBF) in the left frontal lobe. Improved memory quotient (MQ) was significantly correlated with increased rCBF in the left temporal lobe. These findings from Chinese patients confirm a similar regional neuroanatomic dysfunction as in Western patients with the disease.
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