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TK1与LDH联合检测对非霍奇金淋巴瘤鉴别诊断及疗效评估的作用
崔朝杰;邱旸;郝莹莹;刘义庆;田文君;张伟;许瑞;刘春梅;张庆;渠滕;
现代生物医学进展 2019年 19卷18期 页码:3486-3489+3510 影响因子:0.568
Candida Exerts Fluconazole-Resistant Effect via Regulating the Expression of CDR and ERG11 in Patients with Vaginitis
Liu, XH; Meng, YF; Nie, XJ; Li, XY; Tan, LY; Chen, LM
LATIN AMERICAN JOURNAL OF PHARMACY 2021年 40卷8期 页码:1923-
无关键词信息
Vulvovaginitis candidiasis (VVC) is a common fungal infection in women and is usually caused by Candida albicans. Fluconazole is widely used to treat VVC in clinics. However, drug resistance of C. albicans to antifungal azoles is widely developed, which has gradually caused public concerns. This study aimed to investigate the fluconazole resistance of C. albicans and its mechanism. Two hundred and sixty-three yeast isolates were collected from patients with VVC in China and determined their species distribution and susceptibility using the microdilution susceptibility test and Rosco disk diffusion method. Real-time PCR and western blot analyses were used to detect the relevant genes. C. albicans was considered the main yeast in patients with VVC, then C. glabrata, C. tropicalis, etc. Among 175 isolates of C. albicans, 13 isolates were found to have fluconazole resistance (7.43%). Besides, CDR1 was over-expressed in all fluconazole-resistant isolates, while the up-regulation of CDR2 was found in 9 isolates. Moreover, ERG11 was up-regulated in 5 isolates, while MDR1 was increased in 6 isolates, and FLU1 was highly expressed in 6 isolates. In addition, the protein expression of Cdr1 and Cdr2 was generally checked in a fluconazole-resistant isolate, while Cdr2 was not expressed in C3. The fluconazole resistance of vaginal isolates was a complicated problem involved in various mechanisms, which need further studies.
High Neutrophil-lymphocyte Ratio Predicts Serious Renal Insufficiency in Patients with Lupus Nephritis
Tang, DJ; Tang, Q; Zhang, L; Wang, HX
IRANIAN JOURNAL OF IMMUNOLOGY 2022年 19卷1期 页码:49-
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Background: Lupus nephritis (LN) is one of the most serious complications of systemic lupus erythematosus (SLE).The neutrophil to lymphocyte ratio (NLR) is a promising predictor and prognostic factor. An increased NLR is associated with a poor prognosis of several inflammatory diseases. Objective: To evaluate the value of NLR in the diagnosis and pre assessment of the disease severity of LN. Methods: This retrospective study included 88 patients with LN, 51 SLE patients without kidney involvement, 79 patients with primary chronic nephritis (CN), and 52 healthy controls (HC). The differences among these four groups and diagnostic value of NLR for patients with LN were evaluated. Results: The NLR of patients with LN before treatment was significantly higher than that of the other three groups. NLR positively correlated with C-reactive protein (CRP), complement 3(C3), C4, and serum creatinine (SCr) (CRP: r=0.337, p=0.007; C3: r=0.222, p=0.042; C4: r=0.230, p=0.035; SCr: r=0.408, p<0.0001) but negatively correlated with total serum IgG (r=-0.226, p=0.041). The level of NLR increased with the severity of renal dysfunction NLR (area under the curve: 0.785, 95% CI: 0.708-0.862) was useful for the diagnosis of LN, and its optimal cut-off value was 5.44 (sensitivity: 65.9%, specificity: 86.3%). Conclusions: NLR would be useful for the diagnosis of LN and reflects the severity of renal dysfunction Therefore, evaluating NLR before treatment could help clinicians to identify potential renal involvement in patients with SLE and distinguish LN from CN.
One-Pot Identification of BCR/ABL(p210) Transcript Isoforms Based on Nanocluster Beacon
Gou, XL; Xu, LL; Yang, SQ; Cheng, XX; Wu, HP; Zhang, DC; Shi, WC; Ding, SJ; Zhang, YH; Cheng, W
ACS SENSORS 2021年 6卷8期 页码:2928-
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The BCR/ABL(p210) fusion gene is a classic biomarker of chronic myeloid leukemia, which can be divided into e13a2 and e14a2 isoforms according to different breakpoints. These two isoforms showed distinct differences in clinical manifestation, treatment effect, and prognosis risk. Herein, a strategy based on nanocluster beacon (NCB) fluorescence was developed to identify the e13a2 and e14a2 isoforms in one-pot. Because the fluorescence of AgNCs can be activated when they are placed in proximity to the corresponding enhancer sequences, thymine-rich (T-rich) or guanine-rich (G-rich). In this work, we explored an ideal DNA-AgNCs template as an excellent molecular reporter with a high signal-to-noise ratio. After recognition with the corresponding isoforms, the AgNCs can be pulled closer to the T-rich or G-rich sequences to form a three-way junction structure and generate fluorescence with corresponding wavelengths. Therefore, by distinguishing the corresponding wavelengths of AgNCs, we successfully identified two isoforms in one tube with the limitation of 16 pM for e13a2 and 9 pM for e14a2. Moreover, this strategy also realized isoform identification in leukemia cells and newly diagnosed CML patients within 40 min, which provides a powerful tool to distinguish fusion gene subtypes at the same time.
Key gene network related to primary ciliary dyskinesia in hippocampus of patients with Alzheimer's disease revealed by weighted gene co-expression network analysis
Xia, PC; Chen, J; Bai, XH; Li, M; Wang, L; Lu, ZM
BMC NEUROLOGY 2022年 22卷1期 页码:-null
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Background Alzheimer's disease (AD) is closely related to aging, showing an increasing incidence rate for years. As one of the main brain regions involved in AD, hippocampus has been extensively studied due to its association with many human diseases. However, little is known about its association with primary ciliary dyskinesia (PCD). Material and Methods The microarray data of hippocampus on AD were retrieved from the Gene Expression Omnibus (GEO) database to construct the co-expression network by weighted gene co-expression network analysis (WGCNA). The gene network modules associated with AD screened with the common genes were further annotated based on Gene Ontology (GO) database and enriched based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The protein-protein interaction (PPI) network was constructed based on STRING database to identify the hub genes in the network. Results Genes involved in PCD were identified in the hippocampus of AD patients. Functional analysis revealed that these genes were mainly enriched in ciliary tissue, ciliary assembly, axoneme assembly, ciliary movement, microtubule based process, microtubule based movement, organelle assembly, axoneme dynamin complex, cell projection tissue, and microtubule cytoskeleton tissue. A total of 20 central genes, e.g., DYNLRB2, ZMYND10, DRC1, DNAH5, WDR16, TTC25, and ARMC4 were identified as hub genes related to PCD in hippocampus of AD patients. Conclusion Our study demonstrated that AD and PCD have common metabolic pathways. These common pathways provide novel evidence for further investigation of the pathophysiological mechanism and the hub genes suggest new therapeutic targets for the diagnosis and treatment of AD and PCD. Subjects Bioinformatics, Cell Biology, Molecular Biology, Neurology.
Analysis of Eight Nutrient Elements in Whole Blood of Children and Adolescents Using Inductively Coupled Plasma-Mass Spectrometry
Luan, F; Liu, B; Sun, SZ; Chen, Y; Xu, YQ; Jiang, XR; Guo, XR; Cheng, SQ; Wang, Y
BIOLOGICAL TRACE ELEMENT RESEARCH 2021年 页码:-null
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Few researches have been conducted on elements in whole blood of young people. Our study was to investigate the influence of age, gender and season on the contents of magnesium (Mg), calcium (Ca), iron (Fe), copper (Cu), zinc (Zn), manganese (Mn), selenium (Se), and strontium (Sr) as well as to establish reference intervals (RIs). We conducted a retrospective study of 589 apparently healthy children and adolescents. Quantitative analysis had been carried out using inductively coupled plasma-mass spectrometry (ICP-MS). Test results were analyzed using and MannWhitney U test, Spearman and Pearson statistical analyses. RIs were defined by using 95% confidence interval. Differences between contents of Mg, Fe, Cu, and Zn in girls' and boys' whole blood were found. Positive correlations for Fe, Zn, Se, and Sr, while negative for Ca and Cu were found with age. Increasing trends were found for Fe, Zn, and Se, while for Ca and Cu, changes were even decreasing for children and teenagers. The most frequently correlating element pairs were FeZn, MgSe, and FeSe in five successive age groups. Lower contents of Mg, Ca, Fe, Zn, and Se were found in summer. Finally, the reference interval of each element was initially established according to age and gender grouping. The contents of elements in whole blood vary depending mainly on the gender and age of children and adolescents. The reference intervals of elements in whole blood grouped by age and gender provide a reference basis for clinical diagnosis and treatment of element-related diseases.
The Roles of Exosomes as Future Therapeutic Agents and Diagnostic Tools for Glioma
Wu, XB; Wang, XB; Wang, J; Hao, YY; Liu, F; Wang, X; Yang, L; Lu, ZM
FRONTIERS IN ONCOLOGY 2021年 11卷 页码:-null
无关键词信息
Glioma is a common type of tumor originating in the brain. Glioma develops in the gluey supporting cells (glial cells) that surround and support nerve cells. Exosomes are extracellular vesicles that contain microRNAs, messenger RNA, and proteins. Exosomes are the most prominent mediators of intercellular communication, regulating, instructing, and re-educating their surrounding milieu targeting different organs. As exosomes' diameter is in the nano range, the ability to cross the blood-brain barrier, a crucial obstacle in developing therapeutics against brain diseases, including glioma, makes the exosomes a potential candidate for delivering therapeutic agents for targeting malignant glioma. This review communicates the current knowledge of exosomes' significant roles that make them crucial future therapeutic agents and diagnostic tools for glioma.
MicroRNA-22-3p ameliorates Alzheimer's disease by targeting SOX9 through the NF-kappa B signaling pathway in the hippocampus
Xia, PC; Chen, J; Liu, YC; Cui, XL; Wang, CC; Zong, S; Wang, L; Lu, ZM
JOURNAL OF NEUROINFLAMMATION 2022年 19卷1期 页码:-null
无关键词信息
Background: Studies have suggested that many down-regulated miRNAs identified in the brain tissue or serum of Alzheimer's disease (AD) patients were involved in the formation of senile plaques and neurofibrillary tangles. Specifically, our previous study revealed that microRNA-22-3p (miR-22-3p) was significantly down-regulated in AD patients. However, the molecular mechanism underlying the down-regulation of miR-22-3p has not been comprehensively investigated. Methods: The ameliorating effect of miR-22-3p on apoptosis of the A beta-treated HT22 cells was detected by TUNEL staining, flow cytometry, and western blotting. The cognition of mice with stereotaxic injection of agomir or antagomir of miR-22-3p was assessed by Morris water maze test. Pathological changes in the mouse hippocampus were analyzed using hematoxylin and eosin (HE) staining, Nissl staining, and immunohistochemistry. Proteomics analysis was performed to identify the targets of miR-22-3p, which were further validated using dual-luciferase reporter analysis and western blotting analysis. Results: The miR-22-3p played an important role in ameliorating apoptosis in the A beta-treated HT22 cells. Increased levels of miR-22-3p in the mouse hippocampus improved the cognition in mice. Although the miR-22-3p did not cause the decrease of neuronal loss in the hippocampus, it reduced the A beta deposition. Proteomics analysis revealed Sox9 protein as the target of miR-22-3p, which was verified by the luciferase reporter experiments. Conclusion: Our study showed that miR-22-3p could improve apoptosis and reduce A beta deposition by acting on Sox9 through the NF-kappa B signaling pathway to improve the cognition in AD mice. We concluded that miR-22-3p ameliorated AD by targeting Sox9 through the NF-kappa B signaling pathway in the hippocampus.
Clonal Dissemination of Clinical Carbapenem-Resistant Klebsiella pneumoniae Isolates Carrying fosA3 and blaKPC-2 Coharboring Plasmids in Shandong, China
Hao, YY; Zhao, XG; Zhang, C; Bai, YY; Song, Z; Lu, XL; Chen, R; Zhu, YY; Wang, YL
FRONTIERS IN MICROBIOLOGY 2021年 12卷 页码:-null
无关键词信息
Treatment strategies of infection by carbapenem-resistant Klebsiella pneumoniae (CRKP) are limited. Fosfomycin, a broad-spectrum antibiotic, has attracted renewed interest in combination therapy to fight K. pneumoniae infections. However, reports on fosfomycin-resistant K. pneumoniae are increasing. Among the 57 CRKP strains, 40 (70.2%) were resistant to fosfomycin. Thus, whole-genome sequencing and bioinformatics analysis were conducted to reveal molecular characteristics of fosfomycin-resistant K. pneumoniae. Twenty-three isolates coharbored fosA(kp) and fosA3, with K. pneumoniae carbapenemase (KPC)-producing ST11-KL64-wzi64-O2 (n = 13) and ST11-KL47-wzi209-OL101 (n = 8), the predominating clonal groups, while fosA3 was not detected in isolates carrying class B carbapenemase genes. Twenty-two (out of 26) ST11-KL64 strains were positive for rmpA2, of which 12 carried fosA3. Four of the 23 fosA3-positive isolates could successfully transfer their fosfomycin-resistant determinants to Escherichia coli J53Azi(R). All four strains belonged to ST11-KL47 with the same pulsed-field gel electrophoresis profile, and their transconjugants acquired fosfomycin, carbapenem, and aminoglycoside resistance. A 127-kb conjugative pCT-KPC-like hybrid plasmid (pJNKPN52_KPC_fosA) coharboring fosA3, bla(KPC-2), bla(CTX-M-65), bla(SHV-12), rmtB, and bla(TEM-1) was identified. ST11-KL64 and ST11-KL47 K. pneumoniae, with higher resistance and virulence, should be critically monitored to prevent the future dissemination of resistance.
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