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Prognostic value of coronary atherosclerosis progression evaluated by coronary CT angiography in patients with stable angina
Gu, H; Gao, Y; Hou, ZH; Schoepf, UJ; Snyder, AN; Duguay, TM; Wang, XM; Lu, B
EUROPEAN RADIOLOGY 2018年 28卷3期 页码:1066-1076
MULTIDETECTOR COMPUTED-TOMOGRAPHY; ARTERY CALCIUM; INTRAVASCULAR ULTRASOUND; PLAQUE CHARACTERIZATION; CARDIOVASCULAR-DISEASE; MYOCARDIAL-INFARCTION; AMERICAN-COLLEGE; NATURAL-HISTORY; RISK; ASSOCIATION
To investigate the progression of coronary atherosclerosis burden by coronary CT angiography (CCTA) and to demonstrate its association with the incidence of major adverse cardiac events (MACE). We retrospectively studied patients with stable angina who had undergone repeat CCTA due to recurrent or worsening symptoms. Lipid-rich, fibrous, calcified and total plaque burden as well as coronary diameter stenosis were quantitatively analysed. The incidence of MACE during follow-up was determined. The final cohort consisted of 268 patients (mean age 52.9 +/- 9.8 years, 71 % male) with a mean follow-up period of 4.6 +/- 0.9 years. Patients with lipid-rich, fibrous, calcified and total plaque burden (%) progression, as well as coronary diameter stenosis (%) progression had a significantly higher incidence of MACE than those without (all p < 0.05). The progression of lipid-rich plaque (HR = 1.601, p = 0.021), total plaque burden (HR = 2.979, p = 0.043) and coronary diameter stenosis (HR = 4.327, p < 0.001) were independent predictors of MACE (all p < 0.05). Patients presenting with recurrent or worsening symptoms associated with coronary artery disease who have coronary atherosclerosis progression on CCTA are at an increased risk of future MACE. aEuro cent Repeat CCTA can provide information regarding the progression of coronary atherosclerosis. aEuro cent Coronary atherosclerosis progression at CCTA is independently associated with MACE. aEuro cent CCTA findings could serve as incremental predictors of MACE.
The screening and analysis of protein signatures and signaling associated with chemoresistance based on Protein Pathway Array technology in gastric cancer
Lian, GD;Li, LP;Ye, F;Wang, DG;Liu, JL;Shi, YL;Jing, CQ;Suo, J;Zhang, DY;Chen, M
ONCOLOGY REPORTS 2018年 39卷1期 页码:307-315
RANDOMIZED CONTROLLED-TRIAL; ADJUVANT CHEMOTHERAPY; THERAPEUTIC TARGETS; LUNG-CANCER; 5-FLUOROURACIL; RESISTANCE; SURGERY; CELLS; MODEL; PREDICTION
The present study was aimed to identify proteins associated with signaling pathways involved in chemoresistance, and establish a predictive model for chemoresistance in gastric cancer patients after radical surgery. A total of 140 clinically-staged III gastric cancer samples from patients after D2 radical gastrectomy were enrolled in the present study. Protein Pathway Array (PPA) and 286 antibodies were used to assess the protein expression in tumor tissues of patients. The Significance Analysis of Microarray (SAM) software and clustering and discriminant analysis were used to identify differentially expressed proteins between chemosensitive and chemoresistant subsets, and a predictive model for chemoresistance was established using the independent predictive factors. The Ingenuity Pathway Analysis (IPA) software was also used to investigate the relationship between proteins and the signaling transduction network. A total of 23 proteins were differentially expressed between 67 chemosensitive and 73 chemoresitant tumor tissues. Six proteins including PLK1 and DACH1 were independent risk factors for chemoresistance. A predictive model for chemoresistance by these proteins was established, and the accuracy, the sensitivity, and the specificity of this modal was 89.3, 90.3 and 88.2%, respectively. In addition, the present study revealed that differentially expressed proteins were closely related to cellular activity, DNA methylation and DNA damage and repair, and also involved in the ERK/MAPK, Wnt/beta-catenin, PI3K/AKT, apoptosis and p53 signaling pathways. In conclusion, the predictive model established by PPA may be an effective detection system for predicting the chemosensitivity of gastric cancer patients after D2 gastrectomy.
Identification of a novel mutation in the FGFR3 gene in a Chinese family with Hypochondroplasia
Chen, J;Yang, JF;Zhao, SZ;Ying, H;Li, GM;Xu, C
GENE 2018年 641卷 页码:355-360 影响因子:2.319
GROWTH-FACTOR RECEPTOR-3; MISSENSE MUTATIONS; ACHONDROPLASIA; DOMAIN; DYSPLASIAS; PHENOTYPE; DWARFISM
Background: Hypochondroplasia (HCH; OMIM 146000) is a common autosomal dominant skeletal dysplasia characterized by disproportionate short stature, short extremities, relative macrocephaly, and lumbar lordosis. Because of its clinical and genetic heterogeneity, gene mutational analysis is particularly important in diagnosis and the phenotypes may be ameliorated if diagnosed early.;-;Materials and methods: In this study, we examined a Chinese family with HCH, performed an inductive analysis of their clinical features and radiographic results, and applied targeted exome sequencing (TES) technology to perform a molecular diagnosis.;-;Results: The proband and his mother all presented disproportionate short stature, short, stubby extremities, unchanged interpedicular distances from L1 - L5, and short iliac bones, with a 'fish mouth -shaped' sciatic notch. The mother received induced abortion recently because an ultrasound showed short femur length of her fetus at 24-week gestation. Eventually, a novel heterozygous mutation (c.1145G > A) in FGFR3 was identified by TES in the proband, his mother, and her fetus; this causes the substitution of glycine with aspartic acid in codon 382.;-;Conclusions: In this study, we diagnosed a Chinese pedigree with HCH based on clinical data, radiographic features, and genetic testing results. Our results extend the genetic mutation spectrum of FGFR3 and demonstrate that TES is an effective method for the diagnosis of skeletal dysplasia in clinical practices.
Invasive placenta previa: Placental bulge with distorted uterine outline and uterine serosal hypervascularity at 1.5T MRI - useful features for differentiating placenta percreta from placenta accreta
Chen, X;Shan, RQ;Zhao, LX;Song, QX;Zuo, CT;Zhang, XJ;Wang, SS;Shi, HL;Gao, F;Qian, TY;Wang, GB;Limperopoulos, C
EUROPEAN RADIOLOGY 2018年 28卷2期 页码:708-717
ABNORMAL PLACENTATION; ULTRASOUND; DIAGNOSIS; PREGNANCY; SPECTRUM; US
To characterise MRI features of invasive placenta previa and to identify specific features for differentiating placenta percreta (PP) from placenta accreta (PA).;-;Forty-five women with PP and 93 women with PA who underwent 1.5T placental MRI were included. Two radiologists independently evaluated the MRI features of invasive placenta previa, including our novel type of placental bulge (i.e. placental bulge type-II, characterized by placental bulge with distorted uterine outline). Pearson's chi-squared or Fisher's two-sided exact test was performed to compare the MRI features between PP and PA. Logistic stepwise regression analysis and the area under the receiver operating characteristic curve (AUC) were performed to select the optimal features for differentiating PP from PA.;-;Significant differences were found in nine MRI features between women with PP and those with PA (P < 0.05). Placental bulge type-II and uterine serosal hypervascularity were independently associated with PP (odds ratio = 48.618, P < 0.001; odds ratio = 4.165, P = 0.018 respectively), and the combination of the two MRI features to distinguish PP from PA yielded an AUC of 0.92 for its predictive performance.;-;Placental bulge type-II and uterine serosal hypervascularity are useful MRI features for differentiating PP from PA.
Transcriptomic Profiling in Human Decidua of Severe Preeclampsia Detected by RNA Sequencing
Tong, J;Zhao, WX;Lv, H;Li, WP;Chen, ZJ;Zhang, C
JOURNAL OF CELLULAR BIOCHEMISTRY 2018年 119卷1期 页码:607-615
GENE-EXPRESSION; CANCER-CELLS; HYPOXIA; PREGNANCY; GLYCOLYSIS; TISSUE; HIF-1; ADRENOMEDULLIN; PLACENTATION; IMPLANTATION
Maternal decidua plays a critical role in implantation and placentation. Impaired decidualization causes failed intravascular trophoblast invasion and inadequate placentation and then increases the risk of preeclampsia (PE). RNA sequencing (RNA-Seq) has achieved great advances in the characterization and quantification of transcriptomes; is a powerful tool for new transcript discovery, genome annotation, and expression profiling. In the present study, we conducted a RNA-Seq analysis to compare gene expression between decidua of PE (n=3, early-onset severe PE, EOSPE; n=3, late-onset severe PE, LOSPE) and normal pregnancies (n=3). We revealed that decidual gene transcription profile was altered in severe PE and identified 293 key PE-related genes involved in 19 differentially regulated pathways relevant for the pathogenesis of PE, among which ENO2, PGK1, and HK2 involved in glycolysis/gluconeogenesis and HIF-1 signaling pathway which are all highly related with tumorigenesis and are significantly upregulated in cancer cells were severely inhibited in the decidua of PE. Moreover, we identified 22 core regulatory genes, including the newly identified pseudogenes BNIP3P1, HK2P1, and PGK1P1 that encode long non-coding RNA (lncRNA); interestingly, BNIP3/BNIP3P1, HK2/HK2P1, and PGK1/PGK1P1 appear in pairs in core genes. Subsequent analyses using quantitative PCR validated a portion of these results. This study may provide further insight into the mechanisms of PE and function as preventive, predictive, and therapeutic measures. Future functional studies are needed in order to accomplish a greater understanding of the mechanisms involved. J. Cell. Biochem. 119: 607-615, 2018. (c) 2017 Wiley Periodicals, Inc.
Antitumor and DNA Topoisomerase II alpha Inhibitory Activity of 6-Substituted-aryl-2-methoxyquinolines
Li, ZY;Ding, YJ;Bu, HG;Shen, YM
CHINESE JOURNAL OF ORGANIC CHEMISTRY 2018年 38卷12期 页码:3204-3210
无关键词信息
Human DNA Topoisomerase II alpha (Topo II alpha) is one of the important therapeutic targets for the treatment of cancers. Our previous study showed that p-terphenyls have inhibitory effects on Topo II alpha and inhibit the proliferation of human breast ductal carcinoma cells. In this study, nineteen 6-substituted aryl-2-methoxyquinolines (3a similar to 3s) were designed, synthesized and evaluated for their cytotoxicity against the growth of human triple negative breast cancer MDA-MB-231 cell line and inhibitory activity against Topo II alpha. Among these compounds, 6-(4-(hydroxymethyl)phenyl)-2-methoxyquinoline (3b) showed the most potent activity (IC50 = 9.9 mu mol.L-1). These results have important significance for the further study of aryl quinoline TopoII alpha inhibitors.
Preparation of well-distributed titania nanopillar arrays on Ti6Al4V surface by induction heating for enhancing osteogenic differentiation of stem cells
Li, NB;Sun, SJ;Bai, HY;Xiao, GY;Xu, WH;Zhao, JH;Chen, X;Lu, YP;Zhang, YL
NANOTECHNOLOGY 2018年 29卷4期 影响因子:3.573
SIMULATED BODY-FLUID; THERMAL-OXIDATION; OSTEOBLAST DIFFERENTIATION; BIOMEDICAL APPLICATIONS; PROTEIN ADSORPTION; SIGNALING PATHWAY; SELF-RENEWAL; COATINGS; ALLOY; TIO2
Great effort has recently been devoted to the preparation of nanoscale surfaces on titanium-based implants to achieve clinically fast osteoinduction and osseointegration, which relies on the unique characteristics of the nanostructure. In this work, we used induction heating treatment (IHT) as a rapid oxidation method to fabricate a porous nanoscale oxide layer on the Ti6Al4V surface for better medical application. Well-distributed vertical nanopillars were yielded by IHT for 20-35 s on the alloy surface. The composition of the oxides contained rutile/anatase TiO2 and a small amount of Al2O3 between the TiO2 grain boundaries (GBs). This technology resulted in a reduction and subsequent increase of surface roughness of 26-32 nm when upregulating the heating time, followed by the successive enhancement of the thickness, wettability and adhesion strength of the oxidation layer to the matrix. The surface hardness also distinctly rose to 554 HV in the IHT-35 s group compared with the 350 HV of bare Ti6Al4V. The massive small-angle GBs in the bare alloy promoted the formation of nanosized oxide crystallites. The grain refinement and deformation texture reduction further improved the mechanical properties of the matrix after IHT. Moreover, in vitro experiments on a mesenchymal stem cell (BMSC) culture derived from human bone marrow for 1-7 days indicated that the nanoscale layers did not cause cytotoxicity, and facilitated cell differentiation in osteoblasts by enhancing the gene and osteogenesis-related protein expressions after 1-3 weeks of culturing. The increase of the IHT time slightly advanced the BMSC proliferation and differentiation, especially during long-term culture. Our findings provide strong evidence that IHT oxidation technology is a novel nanosurface modification technology, which is potentially promising for further clinical development.
重症医学与信息化
王春亭;栾庆浩
天津医药 2018年 46卷6期 页码:564-567 影响因子:0.655
重症监护病房;;信息学;;重症医学;;大数据;;
重症医学是研究任何损伤或疾病导致机体向死亡发展过程的特点和规律性,并根据这些特点和规律性对重症患者进行治疗的学科.信息化作为名词指的是充分利用信息技术,开发利用信息资源,促进信息和知识共享提高经济增长质量,推动经济社会发展转型的历史进程;作为形容词则指对象或领域因信息技术的深入应用所达成的新形态或状态.重症医学与信息化的结合共分3个阶段:重症医学、信息化;重症医学与信息化;信息化重症医学信息化的发展是为了为人类服务,一定程度上体现为为重症医学服务,而重症医学的发展又肯定了信息化的意义反过来促进信息化的发展,从电子计算机到互联网,到物联网,再到大数据和人工智能,重症医学与信息化的结合正不断翻开新的篇章.
聚桂醇联合平阳霉素、曲安奈德血管瘤内注射疗法对婴幼儿生长发育影响的观察
高华;徐广琪;霍然
中国美容整形外科杂志 2018年 29卷12期 页码:722-725 影响因子:0.547
血管瘤;;聚桂醇;;平阳霉素;;曲安奈德;;
目的 观察联合应用聚桂醇、平阳霉素、曲安奈德血管瘤体内注射治疗婴幼儿血管瘤对儿童生长发育的影响.方法 选择近年来全身不同部位血管瘤患儿90例,采用以上3种药物联合行瘤体内注射治疗.年龄均小于7岁,已经完成治疗不超过3年,且接受注射治疗3次及以上者,性别不限,无其他基础疾病.资料获取采用家庭随访、电话通知复诊和住院患儿调查统计的方法.检查患儿的血常规、肝功能、肺功能、心率、身高、体质量,与正常同龄儿童标准值比较,了解有无异常变化.结果 患儿各项检查指标与正常同龄儿童比较,其差异无统计学意义(P>0.05).结论 联合应用曲安奈德、平阳霉素、聚桂醇瘤体内注射治疗婴幼儿血管瘤,经近3年内观察,无明显毒副作用,对儿童生长发育未发现不良影响.
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